Comparison of two silver staining techniques for detecting lipopolysaccharides in polyacrylamide gels

Journal of Clinical Microbiology

Volumn 40, Issue 11, 2002, Pages 4372-4374

  Tan, Li ^a   Grewal, Parwinder S ^a  

^a OHIO STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LIPOPOLYSACCHARIDE; POLYACRYLAMIDE; SILVER;

ARTICLE; CARBOHYDRATE ANALYSIS; ELECTROPHORESIS; GEL; INTERMETHOD COMPARISON; MORAXELLA; MORAXELLA OSLOENSIS; NONHUMAN; PATHOGENESIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SILVER STAINING;

ANIMALS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; LIPOPOLYSACCHARIDES; MORAXELLA; SENSITIVITY AND SPECIFICITY; SILVER STAINING;

MORAXELLA OSLOENSIS;

EID: 0036841738     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.40.11.4372-4374.2002     Document Type: Article

References (13)

1. Enright, M. C., and H. McKenzie. 1997. Moraxella (Branhamella) catarrhalis: Clinical and molecular aspects of a rediscovered pathogen. J. Med. Microbiol. 46:360-371.
2. Fomsgaard, A., M. A. Freudenberg, and C. Galanos. 1990. Modification of the silver staining technique to detect lipopolysaccharide in polyacrylamide gels. J. Clin. Microbiol. 28:2627-2631.
3. Gu, X.-X., C.-M. Tsai, M. A. Apicella, and D. J. Lim. 1995. Quantitation and biological properties of released and cell-bound lipooligosaccharides from nontypeable Haemophilus influenzae. Infect. Immun. 63:4115-4120.
4. Holme, T., M. Rahman, P. E. Jansson, and G. Widmalm. 1999. The lipopolysaccharide of Moraxella catarrhalis: Structural relationships and antigenic properties. Eur. J. Biochem. 265:524-529.
5. Hoist, O., S. Muller-Loennies, B. Lindner, and H. Brade. 1993. Chemical structure of the lipid A of Escherichia coli J-5. Eur. J. Biochem. 214:695-701.
6. Rietschel, E. T., and O. Luderitz. 1980. Struktur von Lipopolysakkarid und Taxonomie Gram-negativer Bakterien. Forum Mikrobiol. 1:12-20.
7. Takayama, K., N. Qureshi, and P. Mascagni. 1983. Complete structure of lipid A obtained from the lipopolysaccharides of the heptoseless mutant of Salmonella typhimurium. J. Biol. Chem. 258:12801-12803.
8. Tan, L., and P. S. Grewal. 2001. Infection behavior of the rhabditid nematode Phasmarhabditis hermaphrodita to the grey garden slug Deroceras reticulatum. J. Parasitol. 87:1349-1354.
9. Tan, L., and P. S. Grewal. 2001. Pathogenicity of Moraxella osloensis, a bacterium associated with the nematode Phasmarhabditis hermaphrodita, to the slug Deroceras reticulatum. Appl. Environ. Microbiol. 67:5010-5016.
10. Tan, L., and P. S. Grewal. 2002. Endotoxin activity of Moraxella osloensis against the grey garden slug, Deroceras reticulatum. Appl. Environ. Microbiol. 68:3943-3947.
11. Tsai, C., and C. E. Frasch. 1982. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 119:115-119.
12. Vaneechoutte, M., G. Verschraegen, G. Claeys, and A.-M. Van Den Abeele. 1990. Serological typing of Branhamella catarrhalis strains on the basis of lipopolysaccharide antigens. J. Clin. Microbiol. 28:182-187.
13. Westphal, O., and K. Jann. 1965. Bacterial lipopolysaccharides: Extraction with phenol-water and future applications of the procedure. Methods Carbohydr. Chem. 5:83-91.