PI-3 kinase activity is required for epithelial-mesenchymal transformation during palate fusion

Developmental Dynamics

Volumn 225, Issue 3, 2002, Pages 316-321

  Kang, Pei ^a   Svoboda, Kathy K H ^a,b  

^a BAYLOR COLLEGE OF DENTISTRY   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Confocal microscopy; Epithelial mesenchymal transformation (EMT); Palatal fusion score; Palate; PI 3 kinase; TGF 3

Indexed keywords

2 MORPHOLINO 8 PHENYLCHROMONE; CELL SURFACE RECEPTOR; GROWTH FACTOR; LAMININ; PHOSPHATIDYLINOSITOL 3 KINASE; TRANSFORMING GROWTH FACTOR BETA3; 2-(4-MORPHOLINYL)-8-PHENYL-4H-1-BENZOPYRAN-4-ONE; CHROMONE DERIVATIVE; ENZYME INHIBITOR; MORPHOLINE DERIVATIVE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BASEMENT MEMBRANE; CELL MIGRATION; CELL TRANSFORMATION; CLEFT PALATE; CYTOSKELETON; EMBRYO; EMBRYO CULTURE; ENZYME ACTIVITY; EPITHELIUM; EXTRACELLULAR MATRIX; IMMUNOHISTOCHEMISTRY; MESENCHYME; MOUSE; NONHUMAN; PALATE; PHENOTYPE; PRIORITY JOURNAL; SCORING SYSTEM; SIGNAL TRANSDUCTION; ANIMAL; CELL CULTURE; CONFOCAL MICROSCOPY; CYTOLOGY; DRUG ANTAGONISM; ENZYMOLOGY; IN VITRO STUDY; MESODERM; METABOLISM; MOUSE STRAIN; PRENATAL DEVELOPMENT;

1-PHOSPHATIDYLINOSITOL 3-KINASE; ANIMALS; CELLS, CULTURED; CHROMONES; ENZYME INHIBITORS; EPITHELIUM; MESODERM; MICE; MICE, INBRED STRAINS; MICROSCOPY, CONFOCAL; MORPHOLINES; PALATE;

EID: 0036841684     PISSN: 10588388     EISSN: None     Source Type: Journal    
DOI: 10.1002/dvdy.10161     Document Type: Article

References (24)

1. Attisano L, Wrana JL. 2000. Smads as transcriptional co-modulators. Curr Opin Cell Biol 12:235-243.
2. Bakin AV, Tomlinson AK, Bhowmick NA, Moses HL, Arteaga CL. 2000. Phosphatidylinositol 3-kinase function is required for transforming growth factor beta-mediated epithelial to mesenchymal transition and cell migration. J Biol Chem 275:36803-36810.
3. Bhowmick NA, Ghiassi M, Bakin A, Aakre M, Lundquist CA, Engel ME, Arteaga CL, Moses HL. 2001. Transforming growth factorbeta1 mediates epithelial to mesenchymal transdifferentiation through a RhoA-dependent mechanism. Mol Biol Cell 12:27-36.
4. Cui XM, Chai Y, Ito Y, Shuler CF. 2000. Expression of TbetaR4-I and SMAD2 in embryonic palatal tissues. J Dent Res 79:416.
5. Davies SP, Reddy H, Caivano M, Cohen P. 2000. Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem J 351:95-105.
6. Fitchett JE, Hay ED. 1989. Medial edge epithelium transforms to mesenchyme after embryonic palatal shelves fuse. Dev Biol 131:455-474.
7. Griffith CM, Hay ED. 1992. Epithelial-mesenchymal transformation during palatal fusion: Carboxyfluorescein traces cells at light and electron microscopic levels. Development 116:1087-1099.
8. Hannigan GE, Bayani J, Weksberg R, Beatty B, Pandita A, Dedhar S, Squire J. 1997. Mapping of the gene encoding the integrin-linked kinase, ILK, to human chromosome 11p15.5-p15.4. Genomics 42: 177-179.
9. Hay ED. 1995. An overview of epithelio-mesenchymal transformation. Acta Anat 154:8-20.
10. Janji B, Melchior C, Gouon V, Vallar L, Kieffer N. 1999. Autocrine TGF-beta-regulated expression of adhesion receptors and integrin-linked kinase in HT-144 melanoma cells correlates with their metastatic phenotype. Int J Cancer 83:255-262.
11. Martinez-Alvarez C, Tudela C, Perez-Miguelsanz J, O'Kane S, Puerta J, Ferguson MW. 2000. Medial edge epithelial cell fate during palatal fusion. Dev Biol 220:343-357.
12. Metzner B, Barbisch M, Bachmann F, Czech W, Norgauer J. 1996. Evidence of the involvement of phosphatidylinositol 3-kinase in the migration, actin stress fiber formation, and alpha v beta 3-integrin-mediated adherence of human melanoma cells. J Invest Dermatol 107:597-602.
13. Miyazono K. 2000. TGF-beta signaling by Smad proteins. Cytokine Growth Factor Rev 11:15-22.
14. Pece S, Chiariello M, Murga C, Gutkind JS. 1999. Activation of the protein kinase Akt/PKB by the formation of E-cadherin-mediated cell-cell junctions. Evidence for the association of phosphatidylinositol 3-kinase with the E-cadherin adhesion complex. J Biol Chem 274:19347-19351.
15. Radeva G, Petrocelli T, Behrend E, Leung-Hagesteijn C, Filmus J, Slingerland J, Dedhar S. 1997. Overexpression of the integrin-linked kinase promotes anchorage-independent cell cycle progression. J Biol Chem 272:13937-13944.
16. Shuler CF, Halpern DE, Guo Y, Sank AC. 1992. Medial edge epithelium fate traced by cell lineage analysis during epithelial-mesenchymal transformation in vivo. Dev Biol 154:318-330.
17. Smiley GR, Mato M. 1975. A comparison of secondary palate development with different in vitro techniques. Ultrastructural changes in rat palatal epithelium after beta-aminopropionitrile. Anat Rec 181:711-723.
18. Somasiri A, Wu C, Ellchuk T, Turley S, Roskelley CD. 2000. Phosphatidylinositol 3-kinase is required for adherens junction-dependent mammary epithelial cell spheroid formation. Differentiation 66:116-125.
19. Sugiura T, Berditchevski F. 1999. Function of alpha3beta1-tetraspanin protein complexes in tumor cell invasion. Evidence for the role of the complexes in production of matrix metalloproteinase 2 (MMP-2). J Cell Biol 146:1375-1389.
20. Tyler MS. 1975. In vitro development of palatal tissues from embryonic mice. I. Differentiation of the secondary palate from 12-day mouse embryos. Anat Rec 182:297-301.
21. Vlahos CJ, Matter WF, Hui KY, Brown RF. 1994. A specific inhibitor of phosphatidylinositol 3-kinase, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002). J Biol Chem 269:5241-5248.
22. Whitman M. 1998. Smads and early developmental signaling by the TGFbeta superfamily. Genes Dev 12:2445-2462.
23. Wu C, Keightley SY, Leung-Hagesteijn C, Radeva G, Coppolino M, Goicoechea S, McDonald JA, Dedhar S. 1998. Integrin-linked protein kinase regulates fibronectin matrix assembly, E- cadherin expression, and tumorigenicity. J Biol Chem 273:528-536.
24. Zuk A, Hay ED. 1994. Expression of beta 1 integrins changes during transformation of avian lens epithelium to mesenchyme in collagen gels. Dev Dyn 201:378-393.