Cloning and characterization of PRB1, a Candida albicans gene encoding a putative novel endoprotease B and factors affecting its expression

Research in Microbiology

Volumn 153, Issue 9, 2002, Pages 611-620

  Orozco, Iranzu ^a   Ortiz, Lucila ^b   Elorza, Maria Victoria ^a   Ruiz Herrera, José ^b   Sentandreu, Rafael ^a  

^a UNIVERSITY OF VALENCIA   (Spain)

^b DEPARTAMENTO DE INGENIERÍA GENÉTICA   (Mexico)

Author keywords

Candida albicans; Differential display; Factors controlling expression of CaPRB1; Generation of PRB1 by PCR; Overexpressing prb1 strains; PRB1 null mutants, construction

Indexed keywords

CARBON; ENDOPROTEASE B; FUNGAL ENZYME; GENE PRODUCT; N ACETYLGLUCOSAMINE; NITROGEN; PROTEINASE; SUBTILISIN; UNCLASSIFIED DRUG;

ARTICLE; CANDIDA ALBICANS; CHROMOSOME; FUNGAL DEVELOPMENT; FUNGAL GENETICS; FUNGAL STRAIN; GENE EXPRESSION; GENE FREQUENCY; GENE IDENTIFICATION; GENE LIBRARY; GENE MUTATION; GENE PROBE; GENETIC CODE; GENETIC COMPLEMENTATION; HEAT SHOCK; MOLECULAR CLONING; MOLECULAR WEIGHT; MORPHOGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; BLOTTING, SOUTHERN; CANDIDA ALBICANS; CHROMOSOMES, FUNGAL; CLONING, MOLECULAR; DNA, FUNGAL; ELECTROPHORESIS, GEL, PULSED-FIELD; FUNGAL PROTEINS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, FUNGAL; GENE LIBRARY; HUMANS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SERINE ENDOPEPTIDASES;

CANDIDA; CANDIDA ALBICANS; FUNGI; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0036841528     PISSN: 09232508     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0923-2508(02)01373-6     Document Type: Article

References (39)

1. Andaluz E., Ciudad A., Rubio-Coque J., Calderone R., Larriba G. Cell cycle regulation of DNA ligase-encoding gene (CaLIG4) from Candida albicans. Yeast. 15:1999;1199-1210.
2. Ausubel F.M., Brent R., Kingston R.E., Moore D., Seidman J.G., Smith J.A., Struhl K., Current Protocols in Molecular Biology. 1989;Wiley-Interscience, New York.
3. Bertram G., Swoboda R.K., Gooday G.W., Gow N.A.R., Brown A.J.P. Structure and regulation of the Candida albicans ADH1 gene encoding an immunogenic alcohol dehydrogenase. Yeast. 12:1996;115-127.
4. Birse C.E., Irwin M.Y., Fonzi W.A., Sypherd P.S. Cloning and characterization of ECE1, a gene expressed in association with cell elongation of the dimorphic pathogen Candida albicans. Infect. Immun. 61:1993;3648-3655.
5. Blinder D., Magasanik B. Recognition of nitrogen-responsive upstream activation sequences of Saccharomyces cerevisiae by the product of the GLN3 gene. J. Bacteriol. 177:1995;4190-4193.
6. Braun B.R., Johnson A.D. Control of filament formation in Candida albicans by the transcriptional repressor TUP1. Science. 277:1997;105-109.
7. Brown A.J.P., Gow N.A.R. Regulatory networks controlling Candida albicans morphogenesis. Trends Microbiol. 7:1999;333-338.
8. Calderone R.A., Fonzi W.A. Virulence factors of Candida albicans. Trends Microbiol. 9:2001;327-335.
9. Callard D., Lescure B., Mazzolini L. A method for the elimination of falses positives generated by the mRNA differential display technique. Biotechniques. 16:1994;1096-1103.
10. Coffman J.A., el Berry H.M., Cooper T.G. The URE2 protein regulates nitrogen catabolic gene expression through the GATAA-containing UASNTR element in Saccharomyces cerevisiae. J. Bacteriol. 176:1994;7476-7483.
11. Coffman J.A., Cooper T.G. Nitrogen GATA factors participate in transcriptional regulation of vacuolar protease genes in Saccharomyces cerevisiae. J. Bacteriol. 179:1997;5609-5613.
12. Farley P.C., Shepherd M.G., Sullivan P.A. The purification and properties of yeast proteinase B from Candida albicans. Biochem. J. 236:1986;177-184.
13. Feinberg A.P., Vogelstein B. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Addendum. Anal. Biochem. 137:1984;266-267.
14. Fonzi W.A., Irwin M.Y. Isogenic strain construction and gene mapping in Candida albicans. Genetics. 134:1993;717-728.
15. Frederick G.D., Rombouts P., Buxton F.P. Cloning and characterization of PEPC, a gene encoding a serine protease from Aspergillus niger. Gene. 125:1993;57-64.
16. Fujimura H., Sakuma Y. Simplified isolation of chromosomal and plasmid DNA from yeasts. Biotechniques. 14:1993;538-540.
17. Geremia R.A., Goldman G.H., Jacobs D., Ardiles W., Vila S.B., Van Montagu M., Herrera-Estrella A. Molecular characterization of the proteinase-encoding gene, PRB1, related to mycoparasitism by Trichoderma harzianum. Mol. Microbiol. 8:1993;601-603.
18. Gietz D., Woods R.A. High efficiency transformation with lithium acetate. Johnston J.R., Molecular Genetics of Yeast, a Practical Approach. 1994;IRL Press, Oxford.
19. Gillum A.M., Tsay E.Y., Kirsch D.R. Isolation of the Candida albicans gene for orotidine-5 ′ -phosphate decarboxylase by complementation of Saccharomyces cerevisiae ura3 and Escherichia coli pyrF mutations. Mol. Gen. Genet. 198:1984;179-182.
20. Goshorn A.K., Grindle S.M., Scherer S. Gene isolation by complementation in Candida albicans and applications to physical and genetic mapping. Infect. Immun. 60:1992;876-884.
21. Higgins D.G., Sharp P.M. CLUSTAL: A package for performing multiple sequence alignment on a microcomputer. Gene. 73:1988;237-244.
22. Jones E.W. Vacuolar proteases in yeast Saccharomyces cerevisiae. Methods Enzimol. 185:1991;372-386.
23. Jones E.W., Murdock D.G. Proteolysis in the yeast vacuole. Ciechanover A.J., Schwartz A.L., Cellular Proteolytic Systems. 1994;115-134 Wiley-Liss, New York.
24. Langford C.J., Gallwitz D. Evidence for an intron-containing sequence required for the splicing of yeast RNA polymerase II transcripts. Cell. 33:1983;519-527.
25. Li F., Barnathan E.S., Kariko K. Rapid method for screening and cloning cDNA generated in differential display: Application of northern-blot for affinity capturing of cDNAs. Nucleic Acids Res. 22:1994;1764-1765.
26. Liang P., Averboukh L., Pardee A.B. Distribution and cloning of eukaryotic mRNAs by means of differencial display: Refinements and optimisation. Nucleic Acids Res. 21:1993;3269-3275.
27. Lo H.-J., Köhler J.R., DiDomenico B., Loebenberg D., Cacciapuoti A., Fink G.R. Nonfilamentous Candida albicans mutants are avirulent. Cell. 90:1997;939-949.
28. Marzluf G.A. Genetic regulation of nitrogen metabolism in the fungi. Microbiol. Mol. Biol. Rev. 61:1997;17-32.
29. Moehle C.M., Aynardi M.W., Kolodny M.R., Park F.J., Jones E. Protease B of Saccharomyces cerevisiae: Isolation and regulation of the PRB1 structural gene. Genetics. 115:1987;255-263.
30. Moehle C.M., Tizard R., Lemmon S.K., Smart J., Jones E.W. Protease B of the lysosomelike vacuole of the yeast Saccharomyces cerevisiae is homologous to the subtilisin family of serine proteases. Mol. Cell. Biol. 7:1987;4390-4399.
31. Odds F.C., Candida and Candidosis: A Review and Bibliography. 2nd. edn. 1988;Ballière Tindall, London.
32. Pavia J., Aguado C., Mormeneo S., Sentandreu R. Secretion, interaction and assembly of two O-glycosylated cell wall antigens from Candida albicans. Microbiology. 147:2001;1983-1991.
33. Pearson W.R., Lipman D.J. Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA. 85:1988;2444-2448.
34. Portillo F., Gancedo C. Purification and properties of three intracellular proteinases from Candida albicans. Biochem. Biophys. Acta. 881:1986;229-235.
35. Reichard U., Cole G.T., Hill T.W., Ruchel R., Monod M. Molecular characterization and influence on fungal development of ALP2, a novel serine proteinase from Aspergillus fumigatus. Int. J. Med. Microbiol. 290:2000;549-558.
36. Saheki T., Holzer H. Proteolytic activities in yeast. Biochim. Biophys. Acta. 384:1985;203-214.
37. Sambrook J., Fritsch E.F., Maniatis T., Molecular Cloning: A Laboratory Manual. 2nd edn. 1989;Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
38. Siezen R.J., Leunissen J.A.M. Subtilases: The superfamily of subtilisin-like serine proteases. Protein. Sci. 6:1997;501-523.
39. Sterky F., Holmberg A., Pettersson B., Uhlen M. The sequence of a 30 kb fragment on the left arm of chromosome XV from Saccharomyces cerevisiae reveals 15 open reading frames, five of which correspond to previously identified genes. Yeast. 12:1996;1091-1095.