Heterogeneity in the structural dynamics of Sulfolobus solfataricus β-glycosidase revealed by electron paramagnetic resonance and frequency domain fluorometry

Protein Science

Volumn 11, Issue 11, 2002, Pages 2535-2544

  Lozinsky, Evgenia ^a   Febbraio, Ferdinando ^b   Shames, Alexander I ^c   Likhtenshtein, Gertz I ^c   Bismuto, Ettore ^a,b   Nucci, Roberto ^a  

^a INSTITUTE OF PROTEIN BIOCHEMISTRY   (Italy)

^b SECOND UNIVERSITY OF NAPLES   (Italy)

^c BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

Author keywords

Chemical modification; Dynamics; Electron paramagnetic resonance; Frequency domain fluorometry; Mutant

Indexed keywords

BETA GLYCOSIDASE; GLYCOSIDASE; UNCLASSIFIED DRUG; 5,5' DITHIOBIS(2 NITROBENZOIC ACID); CYSTEINE; GLUCOSIDASE; SULFOLOBUS SOLFATARICUS BETA GLYCOSIDASE; SULFOLOBUS SOLFATARICUS BETA-GLYCOSIDASE; THIOL REAGENT;

ARTICLE; CONFORMATIONAL TRANSITION; ELECTRON SPIN RESONANCE; ENZYME STRUCTURE; FLUOROMETRY; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS; SULFOLOBUS SOLFATARICUS; TEMPERATURE DEPENDENCE; CHEMICAL STRUCTURE; CHEMISTRY; CIRCULAR DICHROISM; ENZYMOLOGY; GENETICS; PROTEIN QUATERNARY STRUCTURE; SPIN LABELING; SULFOLOBUS; TEMPERATURE;

SULFOLOBUS; SULFOLOBUS SOLFATARICUS;

CIRCULAR DICHROISM; CYSTEINE; DITHIONITROBENZOIC ACID; ELECTRON SPIN RESONANCE SPECTROSCOPY; FLUOROMETRY; GLUCOSIDASES; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, QUATERNARY; SPIN LABELS; SULFHYDRYL REAGENTS; SULFOLOBUS; TEMPERATURE;

EID: 0036838805     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.0212802     Document Type: Article

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