Evaluation of methods to solubilize and analyze cell-associated ectoenzymes

Journal of Microbiological Methods

Volumn 51, Issue 3, 2002, Pages 273-282

  Konopka, Allan ^a   Zakharova, Taissiia ^a,b  

^a PURDUE UNIVERSITY   (United States)

^b INSTITUTE OF BASIC BIOLOGICAL PROBLEMS   (Russian Federation)

Author keywords

Ectoenzymes; Fluorescent substrate analogs; Gel electrophoresis; Zymogram

Indexed keywords

7 AMINO 4 METHYLCOUMARIN; AMINOPEPTIDASE; COUMARIN DERIVATIVE; DODECYL SULFATE SODIUM; ESTERASE; GLUCOSIDASE; HYDROLASE; HYMECROMONE; LEUCINE; POLYACRYLAMIDE GEL; TRITON X 100; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTEROIDES; CELL FRACTIONATION; CONTROLLED STUDY; CYTOLYSIS; ELECTROPHORETIC MOBILITY; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME SUBSTRATE; FLEXIBACTERACEAE; HIGH TEMPERATURE PROCEDURES; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; STRAIN DIFFERENCE;

AMINOPEPTIDASES; BIOPOLYMERS; BIOREACTORS; CELL FRACTIONATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESTERASES; FLUORESCENT DYES; GLUCOSIDASES; GRAM-NEGATIVE BACTERIA; HYDROLASES; SOLUBILITY;

BACTERIA (MICROORGANISMS); BACTEROIDES; CYTOPHAGA; FLEXIBACTER; GAMMAPROTEOBACTERIA; NEGIBACTERIA; PROTEOBACTERIA;

EID: 0036829408     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(02)00084-2     Document Type: Article

References (25)

1. Blight S.P., Bentley T.L., Lefevre D., Robinson C., Rodrigues R., Rowlands J., Williams P.J.leB. Phasing of autotrophic and heterotrophic plankton metabolism in a temperate coastal ecosystem. Mar. Ecol., Prog. Ser. 128:1995;61-75.
2. Chrost R.J. Microbial ectoenzymes in aquatic environments. Overbeck J., Chrost R.J. Aquatic Microbial Ecology. 1990;47-78 Springer-Verlag, New York.
3. Diaz P., Prim N., Pastor F.I.J. Direct fluroescence-based lipase activity assay. Biotechniques. 27:1999;696-698.
4. Fandino L.B., Riemann L., Steward G.F., Long R.A., Azam F. Variations in bacterial community structure during a dinoflagellate bloom analyzed by DGGE and 16S rDNA sequencing. Aquat. Microbial. Ecol. 23:2001;119-130.
5. Ferro-Luzzi Ames G., Prody C., Kustu S. Simple, rapid, and quantitative release of periplasmic proteins by chloroform. J. Bacteriol. 160:1984;1181-1183.
6. Grossart H.P., Ploug H. Microbial degradation of organic carbon and nitrogen on diatom aggregates. Limnol. Oceanogr. 46:2001;267-277.
7. Hollibaugh J.T., Azam F. Microbial degradation of dissolved proteins in seawater. Limnol. Oceanogr. 28:1983;1104-1116.
8. Hoppe H.G. Use of fluorogenic model substrates for extracellular enzyme activity (EEA) measurement of bacteria. Kemp P.F., Sherr B.F., Sherr E.B., Cole J.J. Handbook of Methods in Aquatic Microbial Ecology. 1993;423-431 Lewis Publishers, Boca Raton.
9. Jones L.J., Upson R.H., Haugland R.P., Panchuk-Voloshina N., Zhou M., Haugland R.P. Quenched BODIPY dye-labeled casein substrates for the assay of protease activity by direct fluorescence measurement. Anal. Biochem. 251:1997;144-152.
10. Konopka A., Knight D., Turco R.F. Characterization of a Pseudomonas sp. capable of aniline degradation in the presence of secondary carbon sources. Appl. Environ. Microbiol. 55:1989;385-389.
11. Konopka A., Zakharova T., Paseuth E., Oliver L., Turco R.F. Physiological state of a microbial community in a biomass recycle reactor. J. Ind. Microbiol. Biotech. 20:1998;23-237.
12. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
13. Leber T.M., Balkwill F.R. Zymography: a single-step staining method for quantitation of proteolytic activity on substrate gels. Anal. Biochem. 249:1997;24-28.
14. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:1951;265-275.
15. Marrs B., Delagrave S., Murphy D. Novel approaches for discovering industrial enzymes. Curr. Opin. Microbiol. 2:1999;241-245.
16. Martinez J., Azam F. Periplasmic aminopeptidase and alkaline phosphatase activities in a marine bacterium: implications for substrate processing in the sea. Mar. Ecol., Prog. Ser. 92:1993;89-97.
17. Münster U. Concentrations and fluxes of organic carbon substrates in the aquatic environment. Antonie van Leeuwenhoek. 63:1993;243-274.
18. Nagata T., Kirchman D.L. Bacterial degradation of protein adsorbed to model submicron particles in seawater. Mar. Ecol., Prog. Ser. 132:1996;241-248.
19. Rath J., Schiller C., Herndl G.J. Ectoenzymatic activity and bacterial dynamics along a trophic gradient in the Caribbean Sea. Mar. Ecol., Prog. Ser. 102:1993;89-96.
20. Riemann L., Steward G.F., Azam F. Dynamics of bacterial community composition and activity during a mesocosm diatom bloom. Appl. Environ. Microbiol. 66:2000;578-587.
21. Salyers A.A., Reeves A., D'Elia J. Solving the problem of how to eat something as big as yourself: diverse bacterial strategies for degrading polysaccharides. J. Ind. Microbiol. 17:1996;470-476.
22. Scholze H., Stütz H., Paltauf F., Hermetter A. Fluorescent inhibitors for the qualitative and quantitative analysis of lipolytic enzymes. Anal. Biochem. 276:1999;72-80.
23. Stevenson F.J. Cycles of Soil: Carbon, Nitrogen, Phosphorus, Sulfur, Micronutrients. The Carbon Cycle. 1986;1-44 Wiley, New York.
24. Tiedje J.M., Asuming-Brempong S., Nüsslein K., Marsh T.L., Flynn S.J. Opening the black box of soil microbial diversity. Appl. Soil Ecol. 13:1999;109-122.
25. Yasothornsrikul S., Hook V.Y.H. Detection of proteolytic activity by fluorescent zymogram in-gel assays. Biotechnology. 28:2000;1166.