A bulged stem tethers Est1p to telomerase RNA in budding yeast

Genes and Development

Volumn 16, Issue 21, 2002, Pages 2800-2812

  Seto, Anita G ^a   Livengood, April J ^a   Tzfati, Yehuda ^b,c   Blackburn, Elizabeth H ^b   Cech, Thomas R ^a  

^a UNIVERSITY OF COLORADO   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

Est1p; RNA structure; Telomerase; TLC1; Yeast

Indexed keywords

NUCLEOTIDE; PROTEIN EST1P; PROTEIN SUBUNIT; RNA; TELOMERASE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DNA SYNTHESIS; GENE OVEREXPRESSION; GENETIC CONSERVATION; IMMUNOPRECIPITATION; KLUYVEROMYCES; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PRIORITY JOURNAL; PROTEIN RNA BINDING; RNA STRUCTURE; SACCHAROMYCES CEREVISIAE; TELOMERE; YEAST;

CONSERVED SEQUENCE; MUTATION; RNA, FUNGAL; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; TELOMERASE;

KLUYVEROMYCES; PROKARYOTA; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

EID: 0036828725     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.1029302     Document Type: Article

References (38)

1. Aigner, S., Lingner, J., Goodrich, K.J., Grosshans, C.A., Shevchenko, A., Mann, M., and Cech, T.R. 2000. Euplotes telomerase contains a La motif protein produced by apparent translational frameshifting. EMBO J. 19: 6230-6239.
2. Allain, F.H., Gubser, C.C., Howe, P.W., and Nagai, K. 1996. Specificity of ribonucleoprotein interaction determined by RNA folding during complex formation. Nature 380: 646-650.
3. Baudin, A., Ozier-Kalogeropoulos, O., Denouel, A., Lacroute, F., and Cullin, C. 1993. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucleic Acids Res. 21: 3329-3330.
4. Chen, J.-L., Opperman, K.K., and Greider, C.W. 2002. A critical stem-loop structure in the CR4-CR5 domain of mammalian telomerase RNA. Nucleic Acids Res. 30: 592-597.
5. Cohn, M. and Blackburn, E.H. 1995. Telomerase in yeast. Science 269: 396-400.
6. Draper, D.E. 1999. Themes in RNA-protein recognition. J. Mol. Biol. 293: 255-270.
7. Evans, S.K. and Lundblad, V. 1999. Estl and Cdcl3 as comediators of telomerase access. Science 286: 117-120.
8. 2002. The Est1 subunit of Saccharomyces cerevisiae telomerase makes multiple contributions to telomere length maintenance. Genetics (In press).
9. Greider, C.W. and Blackburn, E.H. 1989. A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis. Nature 337: 331-337.
10. Hall, K.B. 1994. Interaction of RNA hairpins with the human U1A N-terminal RNA binding domain. Biochemistry 33: 10076-10088.
11. Hughes, T.R., Evans, S.K., Weilbaecher, R.G., and Lundblad, V. 2000. The Est3 protein is a subunit of yeast telomerase. Curr. Biol. 10: 809-812.
12. Juan, V. and Wilson, C. 1999. RNA 'secondary structure prediction based on free energy and phylogenetic analysis. J. Mol. Biol. 289: 935-947.
13. Lai, C.K., Miller, M.C., and Collins, K. 2002. Template boundary definition in Tetrahymena telomerase. Genes & Dev. 16: 514-520.
14. Lendvay, T.S., Morris, D.K., Sah, J., Balasubramanian, B., and Lundblad, V. 1996. Senescence mutants of Saccharomyces cerevisiae with a defect in telomere replication identify three additional EST genes. Genetics 144: 1399-1412.
15. Leulliot, N. and Varani, G. 2001. Current topics in RNA-protein recognition: Control of specificity and biological function through induced fit and conformational capture. Biochemistry 40: 7947-7956.
16. Licht, J.D. and Collins, K. 1999. Telomerase RNA function in recombinant Tetrahymena telomerase. Genes & Dev. 13: 1116-1125.
17. Lingner, J., Cech, T.R., Hughes, T.R., and Lundblad, V. 1997a. Three Ever Shorter Telomere (EST) genes are dispensable for in vitro yeast telomerase activity. Proc. Natl. Acad. Sci. 94: 11190-11195.
18. Lingner, J., Hughes, T.R., Shevchenko, A., Mann, M., Lundblad, V., and Cech, T.R. 1997b. Reverse transcriptase motifs in the catalytic subunit of telomerase. Science 276: 561-567.
19. Livengood, A.J., Zaug, A.J., and Cech, T.R. 2002. Essential regions of Saccharomyces cerevisiae telomerase RNA: Separate elements for Est1p and Est2p interaction. Mol. Cell. Biol. 22: 2366-2374.
20. Lundblad, V. and Szostak, J.W. 1989. A mutant with a defect in telomere elongation leads to senescence in yeast. Cell 57: 633-643.
21. Lundblad, V. and Blackburn, E.H. 1993. An alternative pathway for yeast telomere maintenance rescues est1-senescence. Cell 73: 347-360.
22. Mitchell, J.R. and Collins, K. 2000. Human telomerase activation requires two independent interactions between telomerase RNA and telomerase reverse transcriptase. Mol. Cell 6: 361-371.
23. Mitchell, J.R., Cheng, J., and Collins, K. 1999. A box H/ACA small nucleolar RNA-like domain at the human telomerase RNA 3′ end. Mol. Cell. Biol. 19: 567-576.
24. Nakamura, T.M. and Cech, T.R. 1998. Reversing time: Origin of telomerase. Cell 92: 587-590.
25. Oubridge, C., Ito, N., Evans, P.R., Teo, C.-H., and Nagai, K. 1994. Crystal structure at 1.92 Å resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin. Nature 372: 432-438.
26. Perez-Canadillas, J.-M. and Varani, G. 2001. Recent advances in RNA-protein recognition. Curr. Opin. Struc. Biol. 11: 53-58.
27. Peterson, S.E., Stellwagen, A.E., Diede, S.J., Singer, M.S., Haimberger, Z.W., Johnson, C.O., Tzoneva, M., and Gottschling, D.E. 2001. The function of the stem-loop in telomerase RNA is linked to the DNA repair protein Ku. Nat. Genetics 27: 64-67.
28. Rose, M.D., Winston, F., and Hieter, P. 1990. Methods in Yeast Genetics: A Laboratory Course Manual. pp. 122-123. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
29. Roy, J., Fulton, T.B., and Blackburn, E.H. 1998. Specific telomerase RNA residues distant from the template are essential for telomerase function. Genes & Dev. 12: 3286-3300.
30. Seto, A.G., Zaug, A.J., Sobel, S.G., Wolin, S.L., and Cech, T.R. 1999. Saccharomyces cerevisiae telomerase is an Sm small nuclear ribonucleoprotein particle. Nature 401: 177-180.
31. Singer, M.S. and Gottschling, D.E. 1994. TLC1: Template RNA component of Saccharomyces cerevisiae telomerase. Science 266: 404-409.
32. Sperger, J.M. and Cech, T.R. 2001. A stem-loop of Tetrahymena telomerase RNA distant from the template potentiates RNA folding and telomerase activity. Biochemistry 40: 7005-7016.
33. Taggart, K.P., Teng, S.-C., and Zakian, V.A. 2002. Est1p as a cell cycle-regulated activator of telomere-bound telomerase. Science 297: 1023-1026.
34. Teixeira, M.T., Förstemann, K., Gasser, S.M., and Lingner, J. 2002. Intracellular trafficking of yeast telomerase components. EMBO Rep. 3: 652-659.
35. Teng, S.-C. and Zakian, V.A. 1999. Telomere-telomere recombination is an efficient bypass pathway for telomere maintenance in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 8083-8093.
36. Tzfati, Y., Fulton, T.B., Roy, J., and Blackburn, E.H. 2000. Template boundary in a yeast telomerase specified by RNA structure. Science 288: 863-867.
37. Zhou, J., Hidaka, K., and Futcher, B. 2000. The Est1 subunit of yeast telomerase binds the Tlc1 telomerase RNA. Mol. Cell. Biol. 20: 1947-1955.
38. Zuker, M., Mathews, D.H., and Turner, D.H. 1999. Algorithms and thermodynamics for RNA secondary structure prediction: A practical guide. In RNA Biochemistry and Biotechnology (eds. J. Barciszewski and B.F.C. Clark), pp. 11-43. Kluwer Academic Publishers, Dordrecht, The Netherlands.