Protection of NRK-52E cells, a rat renal proximal tubular cell line, from chemical-induced apoptosis by overexpression of a mitochondrial glutathione transporter

Journal of Pharmacology and Experimental Therapeutics

Volumn 303, Issue 2, 2002, Pages 476-486

  Lash, Lawrence H ^a   Putt, David A ^a   Matherly, Larry H ^a  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; COMPLEMENTARY DNA; CYSTEINE DERIVATIVE; GLUTATHIONE; MALONIC ACID; RECOMBINANT PROTEIN; TERT BUTYL HYDROPEROXIDE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL PROTECTION; CONTROLLED STUDY; DNA SEQUENCE; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE OVEREXPRESSION; IMMUNOBLOTTING; KIDNEY PROXIMAL TUBULE; MALE; MEMBRANE TRANSPORT; MITOCHONDRION; MOLECULAR CLONING; NONHUMAN; OXIDATION REDUCTION STATE; PHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN PURIFICATION; RAT; REGULATORY MECHANISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; APOPTOSIS; BLOTTING, WESTERN; CELL LINE; CYSTEINE; DICARBOXYLIC ACID TRANSPORTERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLOW CYTOMETRY; GLUTATHIONE; KIDNEY TUBULES, PROXIMAL; KINETICS; LIPOSOMES; MALE; MALONATES; MITOCHONDRIA; RATS; RATS, INBRED F344; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TERT-BUTYLHYDROPEROXIDE; TRANSFECTION;

EID: 0036827706     PISSN: 00223565     EISSN: None     Source Type: Journal    
DOI: 10.1124/jpet.102.040220     Document Type: Article

References (38)

1. 2+ by liver mitochondria. J Biol Chem 259:1279-1287.
2. Bisaccia F, Indiveri C, and Palmieri F (1985) Purification of reconstitutively active α-oxoglutarate carrier from pig heart mitochondria. Biochim Biophys Acta 810:362-369.
3. Bisaccia F, Indiveri C, and Palmieri F (1988) Purification and reconstitution of two anion carriers from rat liver mitochondria: The dicarboxylate and 2-oxoglutarate carrier. Biochim Biophys Acta 933:229-240.
4. Chen Y, Cai J, Anders MW, Stevens JL, and Jones DP (2001) Role of mitochondrial dysfunction and S-(1,2-dichlorovinyl)-L-cysteine-induced apoptosis. Toxicol Appl Pharmacol 170:172-180.
5. Chen Z and Lash LH (1998) Evidence for mitochondrial uptake of glutathione by dicarboxylate and 2-oxoglutarate carriers. J Pharmacol Exp Ther 285:608-618.
6. Chen Z, Putt DA, and Lash LH (2000) Enrichment and functional reconstitution of glutathione transport activity from rabbit kidney mitochondria: Further evidence for the role of the dicarboxylate and 2-oxoglutarate carriers in mitochondrial glutathione transport. Arch Biochem Biophys 373:193-202.
7. Current Protocols in Molecular Biology on CD-ROM (1999) John Wiley & Sons, New York.
8. Fernandez-Checa JC, Garcia-Ruiz C, Ookhtens M, and Kaplowitz N (1991) Impaired uptake of glutathione by hepatic mitochondria from ethanol-fed rats. Tracer kinetic studies in vitro and in vivo and susceptibility to oxidant stress. J Clin Invest 87:397-405.
9. Fernandez-Checa JC, Hirano T, Tsukamoto H, and Kaplowitz N (1993) Mitochondrial glutathione depletion in alcoholic liver disease. Alcohol 10:469-475.
10. Fernandez-Checa JC, Kaplowitz N, Garcia-Ruiz C, Colell A, Miranda M, Mari M, Ardite E, and Morales E (1997) GSH transport in mitochondria: Defense against TNF-induced oxidative stress and alcohol-induced defect. Am J Physiol 273:G7-G17.
11. Fiermonte G, Palmieri L, Dolce V, Lasorsa FM, Palmieri F, Runswick MJ, and Walker JE (1998) The sequence, bacterial expression and functional reconstitution of the rat mitochondrial dicarboxylate transporter cloned via distinct homologs in yeast and Caenorhabditis elegans. J Biol Chem 273:24754-24759.
12. Fiermonte G, Walker JE, and Palmieri F (1993) Abundant bacterial expression and reconstitution of an intrinsic membrane-transport protein from bovine mitochondria. Biochem J 294:293-299.
13. Griffith OW and Meister A (1985) Origin and turnover of mitochondrial glutathione. Proc Natl Acad Sci USA 82:4668-4672.
14. Hagen TM, Aw TY, and Jones DP (1988) Glutathione uptake and protection against oxidative injury in isolated kidney cells. Kidney Int 34:74-81.
15. Halestrap AP (1975) The mitochondrial pyruvate carrier: Kinetics and specificity for substrates and inhibitors. Biochem J 148:85-96.
16. Iacobazzi V, De Palma A, and Palmieri F (1996) Cloning and sequencing of the bovine cDNA encoding the mitochondrial tricarboxylate carrier protein. Biochim Biophys Acta 1284:9-12.
17. Kakhniashvili D, Mayor JA, Gremse DA, Xu Y, and Kaplan RS (1997) Identification of a novel gene encoding the yeast mitochondrial dicarboxylate transport protein via overexpression, purification and characterization of its protein product. J Biol Chem 272:4516-4521.
18. Kaplan RS and Pedersen PL (1985) Isolation and reconstitution of the n-butylmalonate-sensitive dicarboxylate transporter from rat liver mitochondria. J Biol Chem 260:10293-10298.
19. Kaplan RS, Mayor JA, Gremse DA, and Wood DO (1995) High level expression and characterization of the mitochondrial citrate transport protein from the yeast Saccharomyces cerevisiae. J Biol Chem 270:4108-4114.
20. Kroemer G, Dallaporta B, and Resche-Rigon M (1998) The mitochondrial death/life regulator in apoptosis and necrosis. Annu Rev Physiol 60:619-642.
21. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond) 227:680-685.
22. Lançar-Benba J, Foucher B, and Saint-Macary M (1994) Purification of the rat-liver mitochondrial dicarboxylate carrier by affinity chromatography on immobilized malate dehydrogenase. Biochim Biophys Acta 1190:213-216.
23. Lash LH and Anders MW (1987) Mechanism of S-(1,2-dichlorovinyl)-L-cysteine- and S-(1,2-dichlorovinyl)-L-homocysteine-induced renal mitochondrial toxicity. Mol Pharmacol 32:549-556.
24. Lash LH and Jones DP (1985) Distribution of oxidized and reduced forms of glutathione and cyst(e)ine in rat plasma. Arch Biochem Biophys 240:583-592.
25. Lash LH, Putt DA, Hueni SE, Cao W, Xu F, Kulidjian SJ, and Horwitz JP (2002) Cellular energetics and glutathione status in NRK-52E cells: Toxicological implications. Biochem Pharmacol, in press.
26. Lash LH and Tokarz JJ (1990) Oxidative stress in isolated rat renal proximal and distal tubular cells. Am J Physiol 259:F338-F347.
27. Lash LH, Visarius TM, Sall JM, Qian W, and Tokarz JJ (1998) Cellular and subcellular heterogeneity of glutathione metabolism and transport in rat kidney cells. Toxicology 130:1-15.
28. Lê-Quôc D and Lê-Quôc K (1989) Relationships between the NAD(P) redox state, fatty acid oxidation and inner membrane permeability in rat liver mitochondria. Arch Biochem Biophys 273:466-478.
29. Lê-Quôc K and Lê-Quôc D (1985) Crucial role of sulfhydryl groups in the mitochondrial inner membrane structure. J Biol Chem 260:7422-7428.
30. Martensson J and Meister A (1989) Mitochondrial damage in muscle occurs after marked depletion of glutathione and is prevented by giving glutathione monoester. Proc Natl Acad Sci USA 86:471-475.
31. McKernan TM, Woods EB, and Lash LH (1991) Uptake of glutathione by renal cortical mitochondria. Arch Biochem Biophys 288:653-663.
32. Palmieri F, Bisaccia F, Capobianco L, Dolce V, Fiermonte G, Iacobazzi V, Indiveri C, and Palmieri L (1996) Mitochondrial metabolite transporters. Biochim Biophys Acta 1275:127-132.
33. Reed DJ (1990) Glutathione: Toxicological implications. Annu Rev Pharmacol Toxicol 30:603-631.
34. Saint-Macary M and Foucher B (1985) Comparative partial purification of the active dicarboxylate transport system of rat liver, kidney and heart mitochondria. Biochem Biophys Res Commun 133:498-504.
35. Schnellmann RG, Gilchrist SM, and Mandel LJ (1988) Intracellular distribution and depletion of glutathione in rabbit renal proximal tubules. Kidney Int 34:229-233.
36. Shan X, Jones DP, Hashmi M, and Anders MW (1993) Selective depletion of mitochondrial glutathione concentrations by (R,S)-3-hydroxy-4-pentenoate potentiates oxidative cell death. Chem Res Toxicol 6:75-81.
37. Smith CV, Jones DP, Guenther TM, Lash LH, and Lauterburg BH (1996) Compartmentation of glutathione: Implications for the study of toxicity and disease. Toxicol Appl Pharmacol 140:1-12.
38. Yagi T and Hatefi Y (1984) Thiols in oxidative phosphorylation: Inhibition and energy-potentiated uncoupling by monothiol and dithiol modifiers. Biochemistry 23:2449-2455.