Sensitivity of multiplex real-time PCR reactions, using the LightCycler and the ABI PRISM 7700 Sequence Detection System, is dependent on the concentration of the DNA polymerase

Molecular and Cellular Probes

Volumn 16, Issue 5, 2002, Pages 351-357

  Exner, M M ^a   Lewinski, M A ^a  

^a QUEST DIAGNOSTICS NICHOLS INSTITUTE   (United States)

Author keywords

ABI PRISM 7700; DNA polymerase; LightCycler; Multiplex PCR; Real time PCR

Indexed keywords

DNA POLYMERASE; GOLD; HLA DQ ANTIGEN; TAQ POLYMERASE; DNA; DNA DIRECTED DNA POLYMERASE;

ACCURACY; ARTICLE; ASSAY; CONCENTRATION (PARAMETERS); CYTOMEGALOVIRUS; DNA HYBRIDIZATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; VIRUS GENOME; ANALYTICAL EQUIPMENT; COMPARATIVE STUDY; DNA SEQUENCE; GENETICS; HUMAN; INSTRUMENTATION; METHODOLOGY; SENSITIVITY AND SPECIFICITY; STANDARD;

CUCUMBER MOSAIC VIRUS;

CYTOMEGALOVIRUS; DNA; DNA-DIRECTED DNA POLYMERASE; HLA-DQ ANTIGENS; HUMANS; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA;

EID: 0036813025     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcpr.2002.0433     Document Type: Article

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