Combined methods of retrograde staining, layer-separation and viscoelastic cell stabilization to isolate retinal ganglion cells in adult rats

Journal of Neuroscience Methods

Volumn 83, Issue 2, 1998, Pages 113-124

  Simon, P ^a   Thanos, S ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Fluorescent dyes; Ganglion cells; Rat retina

Indexed keywords

FLUORESCENT DYE; HYDROXYPROPYLMETHYLCELLULOSE;

ADOLESCENT; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL STRUCTURE; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; RAT; RETINA GANGLION CELL; RETINA INNER PLEXIFORM LAYER; STAINING; VISCOELASTICITY;

AGE FACTORS; ANIMALS; CELL SEPARATION; ELASTICITY; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENT DYES; METHYLCELLULOSE; PROTEASE INHIBITORS; RATS; RATS, SPRAGUE-DAWLEY; RETINAL GANGLION CELLS; SODIUM DODECYL SULFATE; STAINING AND LABELING; STILBAMIDINES; SURFACE-ACTIVE AGENTS;

EID: 0032167582     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(98)00060-0     Document Type: Article

References (26)

1. Armson PF, Bennett MR Neonatal retinal ganglion cell cultures of high purity: effect of superior colliculus on their survival. Neurosci Lett. 38:1983;181-186.
2. Barres BA, Silverstein BE, Corey DP, Chun LL Immunological, morphological, and electrophysiological variation among retinal ganglion cells purified by panning. Neuron. 1:1988;791-803.
3. Barres BA, Schmid R, Sendnter M, Raff MC Multiple extracellular signals are required for long-term oligodendrocyte survival. Development. 118(1):1993;283-295.
4. Barron KD, Dentinger MP, Krohel G, Easton SK, Mankes R Qualitative and quantitative ultrastructural observations on retinal ganglion cell layer of rat after intraorbital optic nerve crush. J Neurocytol. 15:1986;345-362.
5. Beale R, Beaton DW, Neuhoff V, Osborne NN Enriched populations of rat retinal ganglion cells: studies using a cell-type specific surface marker. Neurochem Int. 5:1983;691-696.
6. Bonhoefer F, Huf J In vitro experiments on axon guidance demonstrating an anterior-posterior gradient on the tectum. EMBO J. 1(4):1982;427-431.
7. Bunt AH Ramification patterns of ganglion cell dendrites in the retina of the albino rat. Brain Res. 103:1976;1-8.
8. Dreher B, Sefton AJ, Ni SY, Nisbett G The morphology, number, distribution and central projections of Class I retinal ganglion cells in albino and hooded rats. Brain Behav. Evol. 26:1985;10-48.
9. Fukuda Y A three-group classification of rat retinal ganglion cells: histological and physiological studies. Brain Res. 119:1977;327-334.
10. Henry J-C, Olander K Comparison of the effect of four viscoelastic agents on early postoperative intraocular pressure. J Cataract Refractive Surg. 22(7):1996;960-966.
11. Hong YM, Thanos S A quantitative approach to identify and isolate pure populations of fluorescently labelled adult retinal ganglion cells using a pressure-driven microaspiration technique. Neurosci Lett. 214:1996;111-114.
12. Honig MG, Hume RI Fluorescent carbocyanine dyes allow living neurons of identified origin to be studied in long-term cultures. J Cell Biol. 103:1986;171-187.
13. Lehwalder D, Jeffrey PL, Unsicker K Survival of purified embryonic chick retinal ganglion cells in the presence of neurotrophic factors. J Neurosci Res. 24:1989;329-337.
14. Linden R, Perry VH Massive retinotectal projection in rats. Brain Res. 272:1983;145-149.
15. Mey J, Thanos S Intravitreal injections of neurotrophic factors support the survival of axotomized retinal ganglion cells in adult rats in vivo. Brain Res. 602:1993;304-317.
16. Mey J, Thanos S Functional and biochemical analysis of CNS-relevant neurotrophic activity in the lesioned sciatic nerve of adult rats. J Hirnforsch. 37:1996;25-50.
17. Neukirchen RO, Schlosshauer B, Baars S, Jäckle H, Schwarz U Two-dimensional protein analysis at high resolution on a microscale. J Biol Chem. 257:1982;15229-15234.
18. Perry VH The ganglion cell layer of the retina of the rat: a Golgi study. Proc R Soc London B Biol Sci. 204:1979;363-375.
19. Perry VH Evidence for an amacrine cell system in the ganglion cell layer of the rat retina. Neuroscience. 6:1981;931-944.
20. Richardson PM, Issa VM, Shemie S Regeneration and retrograde degeneration of axons in the rat optic nerve. J Neurocytol. 11:1982;949-966.
21. Sarthy PV, Curtis BM, Catterall WA Retrograde labeling, enrichment and characterization of retinal ganglion cells from the neonatal rat. J Neurosci. 3:1983;2532-2544.
22. Schober W, Gruschka H Die Ganglienzellen der Retina der Albinoratte: Eine qualitative und quantitative Studie. Z mikrosk anat Forschung Leipzig. 91:1977;397-414.
23. Shiosaka S, Kiyama H, Tohyama M A simple method for the separation of retinal sublayers from the entire retina with special reference to application for cell culture. J Neurosci Methods. 10:1984;229-235.
24. Thanos S, Vidal-Sanz M, Aguayo AJ The use of rhodamine-B-isothiocyanate (RITC) as an anterograde and retrograde tracer in the adult rat visual system. Brain Res. 406:1987;317-321.
25. Thanos S, Bähr M, Barde YA, Vanselow J Survival and axonal elongation of adult rat retinal ganglion cells. In vitro effects of lesioned sciatic nerve and brain derived-neurotrophic factor. Eur J Neurosci. 1:1989;19-26.
26. Vidal-Sanz M, Bray GM, Villegas-Perez MP, Thanos S, Aguayo AJ Axonal regeneration and synapse formation in the superior colliculus by retinal ganglion cells in the adult rat. J Neurosci. 7:1987;2894-2909.