Membrane topology inversion of SecG detected by labeling with a membrane-impermeable sulfhydryl reagent that causes a close association of SecG with SecA

Journal of Biochemistry

Volumn 132, Issue 4, 2002, Pages 629-634

  Nagamori, Shushi ^a   Nishiyama, Ken ichi ^a   Tokuda, Hajime ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Cys specific labeling; Membrane protein topology; Protein translocation; SecA; SecG

Indexed keywords

4 ACETAMIDO 4' MALEIMIDYLSTILBENE 2,2' DISULFONIC ACID; ELONGATION FACTOR; PROTEIN; PROTEIN SECA; PROTEINASE; SECG PROTEIN; THIOL DERIVATIVE; UNCLASSIFIED DRUG; 4-ACETAMIDO-4'-MALEIMIDYLSTILBENE-2,2'-DISULFONIC ACID; ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE; BACTERIAL PROTEIN; CARRIER PROTEIN; CROSS LINKING REAGENT; CYSTEINE; ELONGATION FACTOR TU; ESCHERICHIA COLI PROTEIN; MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN A PRECURSOR (E COLI); PROTEIN PRECURSOR; SECA PROTEIN, BACTERIA; SECG PROTEIN, E COLI; STILBENE DERIVATIVE; SULFONIC ACID DERIVATIVE;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CONFORMATIONAL TRANSITION; CROSS LINKING; CYTOPLASM; MEMBRANE PERMEABILITY; MEMBRANE STRUCTURE; PROTEIN INTERACTION; PROTEIN TRANSPORT; SPHEROPLAST; AMINO ACID SEQUENCE; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; CHEMISTRY; CYTOLOGY; ESCHERICHIA COLI; IMMUNOBLOTTING; METABOLISM; MOLECULAR GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; INSERTION SEQUENCES;

ADENOSINE TRIPHOSPHATASES; ADENOSINE TRIPHOSPHATE; AMINO ACID SEQUENCE; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; CROSS-LINKING REAGENTS; CYSTEINE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; IMMUNOBLOTTING; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MOLECULAR SEQUENCE DATA; PEPTIDE ELONGATION FACTOR TU; PROTEIN PRECURSORS; PROTEIN TRANSPORT; SPHEROPLASTS; STILBENES; SULFONIC ACIDS;

EID: 0036775559     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a003266     Document Type: Article

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