The ionic composition of the contractile vacuole fluid of Paramecium mirrors ion transport across the plasma membrane

European Journal of Cell Biology

Volumn 81, Issue 9, 2002, Pages 505-515

  Stock, Christian ^a   Grønlien, Heidi K ^b   Allen, Richard D ^a  

^a UNIVERSITY OF HAWAII   (United States)

^b UNIVERSITY OF OSLO   (Norway)

Author keywords

Contractile vacuole function; Fluid segregation; Intracellular Ca2+ ion selective microelectrodes; Ions in vivo

Indexed keywords

ADENOSINE TRIPHOSPHATASE; CALCIUM; CHLORINE; CHOLINE; HYDROGEN; HYPERTONIC SOLUTION; POTASSIUM; SODIUM; ELECTROLYTE;

ACIDITY; ANIMAL CELL; ARTICLE; CALCIUM TRANSPORT; CELL MEMBRANE POTENTIAL; CELL MEMBRANE TRANSPORT; CELL VACUOLE; CHEMICAL COMPOSITION; CONTROLLED STUDY; CYTOSOL; INTRACELLULAR FLUID; ION TRANSPORT; NONHUMAN; OSMOLARITY; PARAMECIUM; POTASSIUM TRANSPORT; PRIORITY JOURNAL; SEGREGATION ANALYSIS; ANIMAL; CELL MEMBRANE; METABOLISM; PHYSIOLOGY;

ANIMALIA; PARAMECIUM; PARAMECIUM MULTIMICRONUCLEATUM;

ANIMALS; CELL MEMBRANE; CHOLINE; CYTOSOL; ELECTROLYTES; MEMBRANE POTENTIALS; PARAMECIUM; VACUOLES;

EID: 0036749792     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: 10.1078/0171-9335-00272     Document Type: Article

References (39)

1. Allen, R. D., Fok, A. K. (1983): Nonlysosomal vesicles (acidosomes) are involved in phagosome acidification in Paramecium. J. Cell Biol. 97, 566-570.
2. Allen, R. D., Fok, A. K. (1988): Membrane dynamics of the contractile vacuole complex of Paramecium. J. Protozool. 35, 63-71.
3. Allen, R. D., Ueno, M. S., Pollard, L. W., Fok, A. K. (1990): Monoclonal antibody study of the decorated spongiome of contractile vacuole complexes of Paramecium. J. Cell Sci. 96, 469-475.
4. Ammann, D. (1986): Ion-selective microelectrodes. Principles, design and application. Springer-Verlag, Berlin.
5. Baker, P. F. (1976): The regulation of intracellular calcium. Symp. Soc. Exp. Biol. 30, 67-88.
6. Blaustein, M. P. (1974): The interrelationship between sodium and calcium fluxes across cell membranes. Rev. Physiol. Biochem. Pharmacol. 70, 33-82.
7. 2+ homeostasis. Cell Calcium 25, 153-160.
8. Deitmer, J. W., Munsch, T. (1995): Measurement of cytosolic sodium using ion-selective microelectrodes. In: J. Kraicer and S. J. Dixon (eds.): Methods in Neurosciences. Measurement and Manipulation of Intracellular Ions. Academic Press Inc., Orlando, FL, pp. 289-303.
9. Eckert, R. (1972): Bioelectric control of ciliary activity. Science 176, 473-481.
10. 2+ ATPase gene in Paramecium tetraurelia. J. Eukaryot. Microbiol. 44, 250-257.
11. Fok, A. K., Allen, R. D. (1979): Axenic Paramecium caudatum I. Mass culture and structure. J. Protozool. 26, 463-470.
12. Fok, A. K., Aihara, M. S., Ishida, M., Nolta, K. V., Steck, T. L., Allen, R. D. (1995): The pegs on the decorated tubules of the contractile vacuole complex of Paramecium are proton pumps. J. Cell Sci. 108, 3163-3170.
13. Fok, A. K., Yamauchi, K., Ishihara, A., Aihara, M. S., Ishida, M., Allen, R. D. (2002): The vacuolar-ATPase of Paramecium multimicronucleatum: Gene structure of the B subunit and the dynamics of the V-ATPase-rich osmoregulatory membranes. J. Eukaryot. Microbiol. 49, 185-196.
14. Frixione, E., Pérez-Olvera, O. (1985): Contractile vacuole activity in Paramecium aurelia: dependence upon intracellular sodium. Comp. Biochem. Physiol. 82A, 787-790.
15. Grønlien, H. K., Stock, C., Aihara, M., Allen, R. D., Naitoh, Y. (2002): Relating the contractile vacuole complex's membrane potential to its osmoregulatory activity in Paramecium multimicronucleatum. J. Exp. Biol. 205, in press.
16. Hardt, M., Plattner, H. (1999): Quantitative energy-dispersive X-ray microanalysis of calcium dynamics in cell suspensions during stimulation on a subsecond time scale. Preparative and analytical aspects as exemplified by Paramecium cells. J. Struct. Biol. 128, 187-199.
17. Hauser, K., Pavlovic, N., Kissmehl, R., Plattner, H. (1998): Molecular characterization of a sarco(endo)plasmic reticulum ATPase gene from Paramecium tetraurelia and localization of this gene product to subplasmalemmal calcium stores. Biochem. J. 334, 31-38.
18. Herrington, J., Newton, K. R., Bookman, R. J. (1995): Pulse control V4. 6: Igor XOPs for patch clamp data acquisition and capacitance measurements. University of Miami, Miami, FL.
19. Ishida, M., Aihara, M. S., Allen, R. D., Fok, A. K. (1993): Osmoregulation in Paramecium: the locus of fluid segregation in the contractile vacuole complex. J. Cell Sci. 106, 693-702.
20. Ishida, M., Fok, A. K., Aihara, M. S., Allen, R. D. (1996): Hyperosmotic stress leads to reversible dissociation of the proton pump-bearing tubules from the contractile vacuole complex in Paramecium. J. Cell Sci. 109, 229-237.
21. Machemer, H. (1989): Cellular behavior modulated by ions: Electrophysiological implications. J. Protozool. 36, 463-487.
22. 2+-ATPase PAT1 and the contractile vacuole in calcium regulation in Dictyostelium discoideum. J. Cell Sci. 112, 405-414.
23. Murphy, R. F., Powers, S., Cantor, C. R. (1984): Endosome pH measured in single cells by dual fluorescence flow cytometry: rapid acidification of insulin to pH 6. J. Cell Biol. 98, 1757-1762.
24. Naitoh, Y., Eckert, R. (1968a): Electrical properties of Paramecium caudatum: modification by bound and free cations. Z. Vergl. Physiol. 61, 427-452.
25. Naitoh, Y., Eckert, R. (1968b): Electrical properties of Paramecium caudatum: All-or-none electrogenesis. Z. Vergl. Physiol. 61, 453-472.
26. Naitoh, Y., Eckert, R. (1969): Ionic mechanisms controlling behavioral responses of Paramecium to mechanical stimulation. Science 164, 963-965.
27. Naitoh, Y., Kaneko, H. (1972): Reactivated triton-extracted models of Paramecium: Modification of ciliary movement by calcium ions. Science 176, 523-524.
28. 2+-dependent regulation of beat frequency of cilia in Paramecium. J. Cell Sci. 65, 223-231.
29. Oertel, D., Schein, S. J., Kung, C. (1977): Separation of membrane currents using a Paramecium mutant. Nature 268, 120-124.
30. Plattner, H., Habermann, A., Kissmehl, R., Klauke, N., Majoul, I., Söling, H.-D. (1997): Differential distribution of calcium stores in Paramecium cells. Occurrence of a subplasmalemmal store with a calsequestrin-like protein. Eur. J. Cell Biol. 72, 297-306.
31. 2+-ATPase) in subplasmalemmal calcium stores of Paramecium cells. J. Histochem. Cytochem. 47, 841-853.
32. Plattner, H., Klauke, N. (2001): Calcium in ciliated protozoa: sources, regulation, and calcium-regulated cell functions. Int. Rev. Cytol. 201, 115-208.
33. Saimi, Y., Kung, C. (1980): A Ca-induced Na-current in Paramecium. J. Exp. Biol. 88, 305-325.
34. + current shuts off one type of excitation in Paramecium. Proc. Natl. Acad. Sci. USA 80, 5112-5116.
35. Satow, Y., Kung, C. (1980): Membrane currents of pawn mutants of the pwaA group in Paramecium tetraurelia. J. Exp. Biol. 80, 57-71.
36. Stock, C., Allen, R. D., Naitoh, Y. (2001): How external osmolarity affects the activity of the contractile vacuole complex, the cytosolic osmolarity and the water permeability of the plasma membrane in Paramecium multimicronucleatum. J. Exp. Biol. 204, 291-304.
37. Stock, C., Grønlien, H. K., Allen, R. D., Naitoh, Y. (2002): Osmoregulation in Paramecium: in situ ion gradients permit water to cascade through the cytosol to the contractile vacuole. J. Cell Sci. 115, 2339-2348.
38. Stoner, L. C., Dunham, P. B. (1970): Regulation of cellular osmolarity and volume in Tetrahymena. J. Exp. Biol. 53, 391-399.
39. Tominaga, T, Allen, R. D., Naitoh, Y. (1998): Electrophysiology of the in situ contractile vacuole complex of Paramecium reveals its membrane dynamics and electrogenic site during osmoregulatory activity. J. Exp. Biol. 201, 451-460.