The ribosomal translocase homologue Snu114p is involved in unwinding U4/U6 RNA during activation of the spliceosome

EMBO Reports

Volumn 3, Issue 9, 2002, Pages 875-880

  Bartels, Cornelia ^a   Klatt, Christine ^b   Lührmann, Reinhard ^a   Fabrizio, Patrizia ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b PAREXEL INTERNATIONAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELONGATION FACTOR 2; RNA; SMALL NUCLEAR RIBONUCLEOPROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BASE PAIRING; CONTROLLED STUDY; IN VITRO STUDY; IN VIVO STUDY; MUTANT; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN DOMAIN; PROTEIN STABILITY; PROTEIN STRUCTURE; RIBOSOME; RNA SPLICING; RNA STRUCTURE; SACCHAROMYCES CEREVISIAE; SPLICEOSOME; TEMPERATURE SENSITIVITY;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; GENE DELETION; HEAT; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; MUTATION; PHENOTYPE; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RIBOSOMES; RNA, MESSENGER; RNA, SMALL NUCLEAR; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SPLICEOSOMES; TEMPERATURE; TIME FACTORS;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0036747295     PISSN: 1469221X     EISSN: None     Source Type: Journal    
DOI: 10.1093/embo-reports/kvf172     Document Type: Article

References (22)

1. Achsel, T., Ahrens, K., Brahms, H., Teigelkamp, S. and Lührmann, R. (1998) The human U5-22OkD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein. Mol. Cell. Biol., 18, 6756-6766.
2. Ayadi, L., Miller, M. and Banroques, J. (1997) Mutations within the yeast U4/U6 snRNP protein Prp4 affect a late stage of spliceosome assembly. RNA, 3, 197-209.
3. Burge, C.B., Tuschl, T. and Sharp, P.A. (1999) Splicing of precursors to mRNAs by the spliceosomes. In Gesteland, R.F. and Atkins, J.F. (eds), The RNA World. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 525-560.
4. Chen, J.Y., Stands, L., Staley, J.P., Jackups, R.R., Jr, Latus, L.J. and Chang, T.H. (2001) Specific alterations of U1-C protein or U1 small nuclear RNA can eliminate the requirement of Prp28p, an essential DEAD box splicing factor. Mol. Cell, 7, 227-232.
5. Dix, I, Russell, C.S., O'Keefe, R.T., Newman, A.J. and Beggs, J.D. (1998) Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae. RNA, 4, 1239-1250.
6. Fabrizio, P., Laggerbauer, B., Lauber, J., Lane, W.S. and Lührmann, R. (1997) An evolutionarily conserved U5 snRNP-specific protein is a GTP-bindmg factor closely related to the ribosomal translocase EF-2. EMBO J., 16, 4092-4106.
7. Fromont-Racine, M., Rain, J.C. and Legrain, P. (1997) Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens. Nat. Genet., 16, 277-282.
8. Gottschalk, A., Neubauer, G., Banroques, J., Mann, M., Lührmann, R. and Fabrizio, P. (1999) Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP. EMBO J., 18, 4535-4548.
9. Kandels-Lewis, S. and Séraphin, B. (1993) Involvement of U6 snRNA in 5′ splice site selection. Science, 262, 2035-2039.
10. Kuhn, A.N., Li, Z. and Brow, D.A. (1999) Splicing factor Prp8 governs U4/U6 RNA unwinding during activation of the spliceosome. Mol. Cell, 3, 65-75.
11. Laggerbauer, B., Achsel, T. and Lührmann, R. (1998) The human U5-200kD DEXH-box protein unwinds U4/U6 RNA duplices in vitro. Proc. Natl Acad. Sci. USA, 95, 4188-4192.
12. Lesser, C.F. and Guthrie, C. (1993) Mutations in U6 snRNA that alter splice site specificity: implications for the active site. Science, 262, 1982-1988.
13. Nilsen, T.W. (1998) RNA-RNA interactions in nuclear pre-mRNA splicing. In Simons, R. and Grundber-Manago, M. (eds), RNA Structure and Function. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 279-307.
14. Raghunathan, P.L. and Guthrie, C. (1998) RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2. Curr. Biol., 8, 847-855.
15. Sontheimer, E.J. and Steitz, J.A. (1993) The U5 and U6 small nuclear RNAs as active site components of the spliceosome. Science, 262, 1989-1996.
16. Staley, J.P. and Guthrie, C. (1999) An RNA switch at the 5′ splice site requires ATP and the DEAD box protein Prp28p. Mol. Cell, 3, 55-64.
17. Stevens, S.W. and Abelson, J. (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc. Natl Acad. Sci. USA, 96, 7226-7231.
18. Stevens, S.W., Barta, I., Ge, H.Y., Moore, R.E., Young, M.K., Lee, T.D. and Abelson, J. (2001) Biochemical and genetic analyses of the U5, U6, and U4/U6 × U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae. RNA, 7, 1543-1553.
19. Tarn, W.Y., Lee, K.R. and Cheng, S.C. (1993) Yeast precursor mRNA processing protein PRP19 associates with the spliceosome concomitant with or just after dissociation of U4 small nuclear RNA. Proc. Natl Acad Sci. USA, 90, 10821-10825.
20. van Nues, R.W. and Beggs, J.D. (2001) Functional contacts with a range of splicing proteins suggest a central role for Brr2p in the dynamic control of the order of events in spliceosomes of Saccharomyces cerevisiae. Genetics, 157, 1451-1467.
21. Will, C.L. and Lührmann, R. (2001) Spliceosomal UsnRNP biogenesis, structure and function. Curr. Opin. Cell Biol., 13, 290-301.
22. Xie, J., Beickman, K., Otte, E. and Rymond, B.C. (1998) Progression through the spliceosome cycle requires Prp38p function for U4/U6 snRNA dissociation. EMBO J., 17, 2938-2946.