Protein secondary structure and stability determined by combining exoproteolysis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Journal of Mass Spectrometry

Volumn 37, Issue 9, 2002, Pages 974-984

  Villanueva, Josep ^a   Villegas, Virtudes ^a   Querol, Enrique ^a   Avilés, Francesc X ^a   Serrano, Luis ^b  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

Conformation; Exoproteases; Mass spectrometry; Matrix assisted laser desorption ionization time of flight mass spectrometry; Perturbation by chaotropes; Perturbation by mutants; Protein analysis and prediction; Stability; Structure

Indexed keywords

DESORPTION; ENZYMES; GENES; IONIZATION;

PROTEOLYSIS;

MASS SPECTROMETRY;

EXOPEPTIDASE; PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; GENOMICS; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; METHODOLOGY; MUTATION; PREDICTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN FOLDING; PROTEIN LOCALIZATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY;

CARBOXYPEPTIDASES; CARBOXYPEPTIDASES A; ENDOPEPTIDASES; ENZYME PRECURSORS; HYDROLYSIS; MASS SPECTROMETRY; MYOGLOBIN; PEPTIDE FRAGMENTS; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN-TYROSINE KINASES; PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 0036746595     PISSN: 10765174     EISSN: None     Source Type: Journal    
DOI: 10.1002/jms.356     Document Type: Article

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