Transfectant mosaic spheroids: A new model for evaluation of tumour cell killing in targeted radiotherapy and experimental gene therapy

Journal of Gene Medicine

Volumn 4, Issue 5, 2002, Pages 567-576

  Boyd, M ^a   Mairs, S C ^a   Stevenson, K ^a   Livingstone, A ^a,b   Clark, A M ^a   Ross, S C ^a   Mairs, R J ^a,c  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

^b WESTERN INFIRMARY   (United Kingdom)

^c ROYAL HOSPITAL FOR SICK CHILDREN   (United Kingdom)

Author keywords

Bystander effects; Gene therapy; Noradrenaline transporter; Spheroids; Targeted radiotherapy

Indexed keywords

(3 IODOBENZYL)GUANIDINE I 131; COMPLEMENTARY DNA; GREEN FLUORESCENT PROTEIN; NORADRENALIN TRANSPORTER; (3 IODOBENZYL)GUANIDINE; RADIOACTIVE IODINE;

ANIMAL CELL; ARTICLE; BYSTANDER EFFECT; CANCER INHIBITION; CANCER RADIOTHERAPY; CELL HETEROGENEITY; CELL KILLING; CELL POPULATION; CLONOGENIC ASSAY; CONTROLLED STUDY; DRUG CYTOTOXICITY; EXPERIMENTATION; GENE THERAPY; GENETIC CODE; GENETIC TRANSFECTION; IN VITRO STUDY; MARKER GENE; MONOLAYER CULTURE; MOSAICISM; NONHUMAN; PRIORITY JOURNAL; TUMOR CELL; TUMOR CELL LINE; TUMOR MODEL; TUMOR SPHEROID; CELL DEATH; FLOW CYTOMETRY; GENETICS; GLIOMA; HUMAN; METABOLISM; MULTICELLULAR SPHEROID; PATHOLOGY; RADIATION EXPOSURE;

3-IODOBENZYLGUANIDINE; CELL DEATH; FLOW CYTOMETRY; GENE THERAPY; GLIOMA; HUMANS; IODINE RADIOISOTOPES; MOSAICISM; SPHEROIDS, CELLULAR;

EID: 0036726584     PISSN: 1099498X     EISSN: None     Source Type: Journal    
DOI: 10.1002/jgm.293     Document Type: Article

References (28)

1. Henze G, Borgmann A, Von Stackelberg A, Baumgarten E, Uchanska-Ziegler B, Ziegler A, Wittig B. Immunotherapy of acute lymphoblastic leukemia by vaccination with autologous leukemic cells transfected with a cDNA expression plasmid coding for an allogeneic HLA class 1 antigen combined with interleukin-2 treatment. J Mol Med 1998; 76: 215-221.
2. Nakazaki Y, Tani K, Lin ZT, Sumimoto H, Hibino H, Tanabe T, Wu MS, Izawa K, Hase H, Takahashi S, Tojo A, Azuma M, Hamada H, Mori S, Asano S. Vaccine effect of granulocyte-macrophage colony-stimulating factor or CD80 gene-transduced murine hematopoietic tumor cells and their cooperative enhancement of antitumor immunity. Gene Ther 1998; 5 1355-1362.
3. Lawrence TS, Rehemtulla A, Ng EY, Wilson M, Trosko JE, Stetson PL. Preferential cytotoxicity of cells transduced with cytosine deaminase compared to bystander cells after treatment with 5-flucytosine. Cancer Res 1998; 58: 2588-2593.
4. Degreve B, De Clercq E, Balzarini J. Bystander effect of purine nucleoside analogues in HSV-1tk suicide gene therapy is superior to that of pyrimidine nucleoside analogues. Gene Ther 1999; 6 162-170.
5. Spitzweg C, Morris JC. Approaches to gene therapy with sodium/iodide symporter. Exp Clin Endocrinol Diabetes 2001; 109 56-59.
6. 131I meta-iodobenzylguanidine. Gene Ther 1999; 6: 1147-1152.
7. 131I]MIBG. J Gene Med 2001; 3: 165-172.
8. 131I]iodobenzylguanidine. J Nucl Med 1980; 21: 349-353.
9. Jacques S Jr, Tobes MC, Sisson JC, Baker JA, Weiland DM. Comparison of the sodium dependence of uptake of meta-iodobenzylguanidine and norephrine into cultured bovine adrenomedullary cells. Mol Pharmacol 1984; 26: 539-546.
10. 131I]MIBG and clonogenic cell kill. Oncogene 2001; 20: 7804-7808.
11. Boyd M, Livingston A, Wilson L, Marshall AG, McCluskey AG, Mairs RJ, Wheldon TE. Dose-response relationship for radiation-induced mutations at micro- and minisatellite loci in human somatic cells in culture. Int J Radiat Biol 1999; 76: 169-176.
12. Lingen B, Bruss M, Bonisch H. Cloning and expression of the bovine sodium- and chlorine-dependent noradrenaline transporter. FEBS Lett 1994; 342: 235-238.
13. Wheldon TE, Brunton GF. Relation of growth delay to cure for experimental tumour systems conforming to poisson cure statistics. Br J Cancer 1982; 45: 1-9.
14. 131I-meta-iodobenzylguanidine therapy in neuroblastoma spheroids of different sizes. Br J Cancer 1992; 66: 1408-1052.
15. Wheldon TE, Livingstone A, Wilson L. The radiosensitivity of the human neuroblastoma cells estimated from regrowth curves of multicellular tumour spheroids. Br J Radiol 1985; 58: 661-664.
16. Schwachofer JH, Crooijmans RP, Broers JL, et al. Multicellular aggregates from human tumour cell lines for radiation studies. Anticancer Res 1989; 9: 441-448.
17. Djordjevic B, Lange CS. Clonogenicity of mammalian cells in hybrid spheroids: a new assay method. Radiat Environ Biophys 1990; 29: 31-46.
18. Bishayee A, Rao DV, Howell RW. Evidence for pronounced bystander effects caused by nonuniform distributions of radioactivity using a novel three-dimensional tissue culture model. Radiat Res 1999; 152: 88-97.
19. Cunningham SH, Mairs RJ, Wheldon TE, Welsh PC, Vaidyanathan G, Zalutsky MR. Radiotoxicity to neuroblastoma cells and spheroids of beta-, alpha- and Auger electron-emitting conjugates of benzylguanidine. Br J Cancer 1998; 77: 2061-2068.
20. 131I]IUDR to human glioma cells in monolayer or spheroid culture: effect of proliferative heterogeneity and radiation cross-fire. Br J Cancer 1998; 77: 385-390.
21. Sigg M, Crompton NE, Burkart W. Enhanced neoplastic transformation in an inhomogeneous radiation field: an effect of the presence of heavily damaged cells. Radiat Res 1997; 148: 543-547.
22. Mothersill C, Seymore CB. Cell-cell contact during gamma irradiation is not required to induce a bystander effect in normal human keratinocytes: evidence for release during irradiation of a signal controlling survival into the medium. Radiat Res 1998; 149: 256-262.
23. Azzam EL, de Toledo SM, Gooding T, Little JB. Intercellular communication is involved in the bystander regulation of gene expression in human cells exposed to very low fluences of alpha particles. Radiat Res 1998; 150: 497-504.
24. Nagasawa H, Little JB. Unexpected sensitivity to the induction of mutations by very low doses of alpha-particle radiation: evidence for a bystander effect. Radiat Res 1999; 152: 552-557.
25. 131I]iodobenzylguanidine. Appl Radiat Isot 1993; 621-628.
26. Mairs RJ, Zalutsky MR. New MIBG preparation to improve targeted radiotherapy and reduce toxic side-effects in neuroblastoma patients undergoing combination treatment. Br J Cancer 1995; 72 250.
27. Strickland DK, Vaidyanathan G, Zalutsky MR. Cytotoxicity of α-particle emitting m-[At]astobenzylguanidine on human neuroblastoma cells. Cancer Res 1994; 54: 5414-5419.
28. Charlton DE. Radiation effects in spheroids of cells exposed to alpha emitters. Int J Radiat Biol 2000; 76: 1555-1564.