Homomultimerization of the coxsackievirus 2B protein in living cells visualized by fluorescence resonance energy transfer microscopy

Journal of Virology

Volumn 76, Issue 18, 2002, Pages 9446-9456

  Van Kuppeveld, Frank J M ^a   Melchers, Willem J G ^a   Willems, Peter H G M ^a   Gadella Jr , Theodorus W J ^a  

^a RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PROTEIN 2B; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; COXSACKIE VIRUS; FLUORESCENCE MICROSCOPY; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE;

ANIMALS; BACTERIAL PROTEINS; CELL LINE; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; DIMERIZATION; ENERGY TRANSFER; ENTEROVIRUS B, HUMAN; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE; TRANSFECTION; VIRAL PROTEINS;

EID: 0036721022     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.76.18.9446-9456.2002     Document Type: Article

References (37)

1. Membrane permeabilization by poliovirus proteins 2B and 2BC
2. Induction of membrane proliferation by poliovirus proteins 2C and 2BC
3. Fluorescence lifetime imaging microscopy: Spatial resolution of biochemical processes in the cell
4. The structure, dynamics and orientation of antimicrobial peptides in membranes by multidimensional solid-state NMR spectroscopy
5. Genetic complementation among poliovirus mutants derived from an infectious cDNA clone
6. Characteristics of the poliovirus replication complex
7. Intracellular distribution of poliovirus proteins and the induction of virus-specific cytoplasmic structures
8. Modification of membrane permeability by animal viruses
9. Membrane rearrangement and vesicle induction by recombinant poliovirus 2C and 2BC in human cells
10. Fluorescence resonance energy transfer
11. Multimerization reactions of the coxsackievirus proteins 2B, 2C, and 2BC: A mammalian two-hybrid analysis
12. Inhibition of cellular protein secretion by poliovirus proteins 2B and 3A
13. Fluorescence lifetime imaging microscopy (FLIM): Instrumentation and applications
14. Fluorescence lifetime imaging microscopy (FLIM)-spatial resolution of microstructures on the nanosecond time-scale
15. GFP-based FRET microscopy in living plant cells
16. Microspectroscopic imaging of nodulation factor-binding sites on living Vicia sativa roots using a novel bioactive fluorescent nodulation factor
17. Fluorescence lifetime imaging microscopy: Pixel-by-pixel analysis of phase modulation data
18. Construction and characterization of a frequency domain fluorescence lifetime imaging microscopy system
19. Advanced microspectroscopic methods for monitoring fluorescent molecules in living plant root hairs
20. Analysis of MADS box protein-protein interactions in living plant cells
21. Three poliovirus 2B mutants exhibit noncomplementable defects in viral RNA amplification and display dosage-dependent dominance over wild-type poliovirus
22. 2+ measurements using improved cameleons
23. Using GFP in FRET-based applications
24. Poliovirus infection and expression of the poliovirus 2B provoke the disassembly of the Golgi complex, the organelle target for the antipoliovirus drug Ro-090179
25. Amphipathic helix motif: Classes and properties
26. The renaissance of fluorescence resonance energy transfer
27. Subcellular localization and oligomerization of the Arabidopsis thaliana somatic embryogenesis receptor kinase 1 protein
28. Mechanism of the binding, insertion and destabilization of phospholipid bilayer membranes by α-helical antimicrobial and cell nonselective membrane-lytic peptides
29. Three dimensional image restoration in fluorescence lifetime imaging microscopy
30. Dimerization of receptor protein-tyrosine phosphatase alpha in living cells
31. The green fluorescent protein
32. Genetic analysis of a hydrophobic domain of coxsackie B3 virus protein 2B; a moderate degree of hydrophobicity is required for a cis-acting function in viral RNA synthesis
33. Coxsackie B3 virus protein 2B contains a cationic amphipathic helix that is required for viral RNA replication
34. Coxsackievirus protein 2B modifies endoplasmic reticulum membrane and plasma membrane permeability and facilitates virus release
35. Structure-function analysis of coxsackie B3 virus protein 2B
36. Chimeric coxsackie B3 virus genomes that express hybrid coxsackievirus-poliovirus 2B proteins: Functional dissection of structural domains involved in RNA replication
37. Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells