Chimeric coxsackie B3 virus genomes that express hybrid coxsackievirus-poliovirus 2B proteins: Functional dissection of structural domains involved in RNA replication

Journal of General Virology

Volumn 78, Issue 8, 1997, Pages 1833-1840

  Van Kuppeveld, Frank J M ^a   Van Den Hurk, Patrick J J C ^a   Van Der Vliet, Walter ^a   Galama, Jochem M D ^a   Melchers, Willem J G ^a  

^a RADBOUD UNIVERSITY NIJMEGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; HYBRID PROTEIN; VIRUS PROTEIN; VIRUS RNA;

ALPHA HELIX; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; COXSACKIE VIRUS B3; NONHUMAN; POLIOMYELITIS VIRUS; PRIORITY JOURNAL; PROTEIN DOMAIN; RNA REPLICATION; STRUCTURE ACTIVITY RELATION; VIRUS GENOME;

COXSACKIEVIRUS; ENTEROVIRUS; POLIOVIRUS;

EID: 0030839598     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-78-8-1833     Document Type: Article

References (30)

1. Agol, V. I., Pilipenko, E. V. & Slobodskaya, O. R. (1996). Modification of translational control elements as a new approach to design of attenuated picornavirus strains. Journal of Biotechnology 44, 119-128.
2. Alexander, L., Lu, H. H. & Wimmer, E. (1994). Polioviruses containing picornavirus type 1 and/or type 2 internal ribosome entry site elements: genetic hybrids and the expression of a foreign gene. Proceedings of the National Academy of Sciences, USA 91, 1406-1410.
3. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, O. D., Seidman, J. G., Smith, J. A. & Struhl, K. (editors) (1990). Current Protocols in Molecular Biology, vol. 1. New York: John Wiley.
4. Barco, A. & Carrasco, L. (1995). A human virus protein, poliovirus protein 2BC, induces membrane proliferation and blocks the exocytic pathway in the yeast Saccharomyces cerevisiae. EMBO Journal 14, 3349-3364.
5. Bernheimer, A. W. & Rudy, B. (1986). Interactions between membranes and cytolytic peptides. Biochimica et Biophysica Acta 864, 123-141.
6. Bienz, K., Egger, D., Rasser, Y. & Bossart, W. (1983). Intracellular distribution of poliovirus proteins and the induction of virus-specific cytoplasmic structures. Virology 131, 39-48.
7. Bienz, K., Egger, D. & Pfister, T. (1994). Characteristics of the poliovirus replication complex. Archives of Virology supplement 9, 147-157.
8. Carrasco, L., Pérez, L., Irurzun, A., Lama, J., Martínez-Abarca, F., Rodríguez, P., Guinea, R., Castrillo, J. L., Sanzm, M. A. & Ayala, M. J. (1993). Modification of membrane permeability by animal viruses. In Regulation of Gene Expression in Animal Viruses, pp. 283-305. Edited by L. Carrasco, N. Sonenberg & E. Wimmer. New York: Plenum Press.
9. Chomczynski, P. & Sacchi, N. (1987). Single step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162, 156-159.
10. Dewalt, P. G., Lawson, M. A., Colonno, R. J. & Semler, B. L. (1989). Chimeric picornavirus polyproteins demonstrate a common 3C proteinase substrate specificity. Journal of Virology 63, 3444-3452.
11. Doedens, J. R. & Kirkegaard, K. (1995). Inhibition of cellular protein secretion by poliovirus proteins 2B and 3A. EMBO Journal 14, 894-907.
12. Hyypiä, T., Hovi, T., Knowles, N. J. & Stanway, G. (1997). Classification of enteroviruses based on molecular and biological properties. Journal of General Virology 78, 1-11.
13. Johnson, K. L. & Sarnow, P. (1991). Three poliovirus 2B mutants exhibit non-complementable defects in viral RNA amplification and display dosage-dependent dominance over wild-type poliovirus. Journal of Virology 65, 4341-4349.
14. Klump, W. M., Bergman, I., Müller, B. C., Ameis, D. & Kandolf, R. (1990). Complete nucleotide sequence of infectious coxsackievirus B3 cDNA: two initial 5′ uridine residues are regained during plus-strand RNA synthesis. Journal of Virology 64, 1573-1583.
15. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
16. Lama, J. & Carrasco, L. (1992). Expression of poliovirus nonstructural proteins in Escherichia colt cells. Journal of Biological Chemistry 267, 15932-15937.
17. Li, J.-P. & Baltimore, D. (1988). Isolation of poliovirus mutants defective in viral RNA synthesis. Journal of Virology 62, 4016-4021.
18. pro proteins: separation of proteinase from translation and replication functions. Journal of Virology 69, 7445-7452.
19. Melnick, J. L. (1996). Enteroviruses: polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses. In Fields Virology, 3rd edn, vol. 1, pp. 655-712. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia: Lippincott-Raven.
20. Racaniello, V. R. (1990). Cell receptors for picomaviruses. Current Topics in Microbiology and Immunology 161, 1-22.
21. Reed, L. J. & Muench, H. (1938). A simple method of estimating fifty per cent endpoints. American journal of Hygiene 27, 493-497.
22. Rohll, J. B., Percy, N., Ley, R., Evans, D. J., Almond, J. F. & Barclay, W. S. (1994). The 5′-untranslated region of picomavirus RNA contain independent functional domains essential for RNA replication and translation. Journal of Virology 68, 4383-4391.
23. Shai, Y. (1995). Molecular recognition between membrane-spanning polypeptides. Trends in Biological Sciences 20, 460-464.
24. van Kuppeveld, F. J. M., Galama, J. M. D., Zoll, J. & Melchers, W. J. G. (1995). Genetic analysis of a hydrophobic domain of coxsackie B3 virus protein 2B; a moderate degree of hydrophobicity is required for a cis-acting function in viral RNA synthesis. Journal of Virology 69, 7782-7790.
25. van Kuppeveld, F. J. M., Galama, J. M. D., Zoll, J., van den Hurk, P. J. J. C. & Melchers, W. J. G. (1996a). Coxsackie B3 virus protein 2B contains a cationic amphipathic helix that is required for viral replication. Journal of Virology 70, 3876-3886.
26. van Kuppeveld, F. J. M., van den Hurk, P. J. J. C., Zoll, J., Galama, J. M. D. & Melchers, W. J. G. (1996b). Mutagenesis of the coxsackie B3 virus 2B/2C cleavage site: determinants of processing efficiency and effects on viral replication. Journal of Virology 70, 7632-7640.
27. van Kuppeveld, F. J. M., Melchers, W. J. G., Kirkegaard, K. & Doedens, J. R. (1997a). Structure-function analysis of coxsackie B3 virus protein 2B. Virology 227, 111-118.
28. van Kuppeveld, F. J. M., Hoenderop, J. G. J., Smeets, R. L. L., Willems, P. H. G. M., Dijkman, H. B. P. M., Galama, J. M. D. & Melchers, W. J. G. (1997b). Coxsackie-virus protein 2B modifies the ER membrane and plasma membrane permeability and facilitates virus release. EMBO Journal (in press).
29. Wimmer, E., Helen, C. U. T. & Cao, X. (1993). Genetics of poliovirus. Annual Review of Genetics 27, 353-436.
30. pro of poliovirus is essential for RNA replication. Journal of Virology 69, 3658-3667.