Sodium chloride, osmolyte, and osmolarity effects on blastocyst formation in bovine embryos produced by in vitro fertilization (IVF) and cultured in simple serum-free media

Journal of Assisted Reproduction and Genetics

Volumn 13, Issue 7, 1996, Pages 562-568

  Liu, Zishu ^a   Foote, Robert H ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

Bovine embryos; In vitro fertilization; KSOM; NaCl; Osmolarity

Indexed keywords

BETAINE; INOSITOL; SODIUM CHLORIDE; SORBITOL;

ANIMAL CELL; ARTICLE; BLASTOCYST; CATTLE; EMBRYO; EMBRYO CULTURE; EMBRYO DEVELOPMENT; FERTILIZATION IN VITRO; NONHUMAN; OSMOLARITY; PRIORITY JOURNAL; ZYGOTE;

ANIMALS; BETAINE; BLASTOCYST; CATTLE; CULTURE MEDIA, SERUM-FREE; EMBRYONIC AND FETAL DEVELOPMENT; FERTILIZATION IN VITRO; INOSITOL; OSMOLAR CONCENTRATION; SODIUM CHLORIDE; SORBITOL; ZYGOTE;

EID: 0029844561     PISSN: 10580468     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02066609     Document Type: Article

References (38)

1. Biggers JD, Brinster RL: Biometrical problems in the study of early mammalian embryos in vitro. J Exp Zool 1965;158:39-48
2. Naglee DL. Maurer RA, Foote RH: Effect of osmolarity on in vitro development of rabbit embryos in a chemically defined medium. Exp Cell Res 1969;58:331-333
3. Whitten WK: Nutrient requirements for the culture of preimplanlation embryos in vitro. Adv Biosci 1971;6:129-141
4. Lim JM, Kim JH, Okuda K. Niwa K: The importance of NaCl concentration in a chemically defined medium for the development of bovine oocytes matured and fertilized in vitro. Theriogenology 1994;42:421-432
5. Miyoshi K. Funahashi H, Okuda K, Niwa K: Development of rat one cell embryos in a chemically defined medium: Effect of glucose, phosphate and osmolarity. J Reprod Fertil 1994; 100:21-26
6. Pinyopummintr T. Bavister BD: In vitro matured/in vitro fertilized bovine oocytes can develop into morulae/blastocysts in chemically defined protein-tree culture medium. Biol Reprod 1991;45:736-742
7. Gardner DK, Lane M, Spitzer A, Batt PA: Enhanced rates of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum and somatic cells: Amino acids, vitamins, and culturing embryos in groups stimulate development. Biol Reprod 1994;50:390-400
8. 2. J Reprod Dev 1994;40:1-9
9. Lawitts JA. Biggers JD: Overcoming the 2-cell block by modifying components in a mouse embryo culture medium. Biol Reprod 1991;45:245-251
10. Beckmann LS. Day BN: Effects of media, NaCl concentration and osmolarity on the culture of early-stage porcine embryos and the viability of embryos cultured in a selected superior medium. Theriogenology 1993;39:611-622
11. Ho Y, Doherty AS, Schultz RM: Mouse preimplantation embryo development in vitro: Effect of sodium concentration in culture media on RNA synthesis and accumulation and gene expression. Mol Reprod Dev 1994;38:131-141
12. Anbari K, Schultz RM: Effect of sodium and betaine in culture media on development and relative rates of protein synthesis in preimplantation mouse embryos in vitro. Mol Reprod Dev 1993;35:24-28
13. Biggers JD. Lawitts JA, Lechene CP: The protective action of betaine on the deleterious effects of NaCl on preimplantation mouse embryos in vitro. Mol Reprod Dev 1993;34:380-390
14. Kane MT, Bavister B: Vitamin requirements for development ot eight-cell hamster embryos to hatching blastocysts in vitro. Biol Reprod 1988;39:1137-1143
15. Kane MT: Effects of putative phospholipid precursors, inositol. choline, serine and ethanolamine, on formation and expansion of rabbit blastocysts in vitro. J Reprod Fertil 1989;87:275-279
16. Fahy MM, Kane MT: Fnositol stimulates DNA and protein synthesis, and expansion by rabbit blastocysts in vitro. Hum Reprod 1992;7:550-552
17. Li J, Foote RH: Effect of inositol with increasing sodium chloride and constant osmolality on development of rabbit embryos. J Assist Reprod Genet 1995;12:141-146
18. Bredbacka P, Bredbacka K: Effect of inositol on in vitro development of murine and bovine morulae and blastocysts, Theriogenology 1993;39:195 (abstr)
19. Bavister BD, Leibfried ML, Lieberman G: Development of preimplantation embryos of the golden hamster in a defined culture medium. Biol Reprod 1983;28:235-247
20. Kane MT, Foote RH: Culture of two- and four-cell rabbit embryos to the expanding blastocyst stage in synthetic media. Proc Soc Exp Biol Med 1970;133:921-925
21. Quinn, P: Enhanced results in mouse and human embryo culture using a modified human tubal fluid medium lacking glucose and phosphate. J Assist Reprod Genet 1995;12:97-105
22. Brackett BG, Oliphant G: Capacitation of rabbit spermatozoa in vitro. Biol Reprod 1975;12:260-274
23. 2. Biol Reprod 1995;53:786-790
24. Lawitts JA, Biggers JD: Culture of preimplantation embryos methods in enzymology. In Guide to Techniques in Mouse Development. PM Wassarman, ML DePamphilis (eds). San Diego, Academic Press, 1993, pp 153-164
25. Erbach GT, Lawitts JA, Papaioannou VE, Biggers JD: Differential growth of the mouse preimplantalion embryo in chemicaily defined media, Biol Reprod 1994;50:1027-1033
26. Brinster RL: Studies on the development of mouse embryo in vitro. Effect of osmolarily and hydrogen ion concentration. J Exp Zool 1965;158:49-58
27. MeKiernan SH, Bavister BD: Environmental variables influencing in vitro development of hamster 2-cell embryos to the blastocyst stage. Biol Reprod 1990;43:404-413
28. Lawitts JA, Biggers JD: Joint effects of sodium chloride, glutamine, and glucose in mouse preimplantation embryos culture media. Mol Reprod Dev 1992;31:189-194
29. Biggers JD, Bell JE, Benos DJ: Mammalian blastocyst: Transport functions in a developing epithelium. Am J Physiol 1988;255:c419-c432
30. Manejwara FW, Cragoe EJ, Schultz RM: Blastocoeleexpansion in the preimplantation mouse embryo: role of extracellular sodium and chloride and possible apical routes of their entry. Dev Biol 1989;133:210-220
31. Brison JD, Leese HJ: Role of chloride transport in the development of the rat blastocyst. Biol Reprod 1993;48:692-702
32. Kane MT, Morris M, Harrison RAP: Uptake and incorporation of inositol by preimplantation mouse embryos. J Reprod Fertil 1992;96:617-625
33. 3H)inositol into phosphoinositides and inositol phosphates by rabbit blastocysts. Mol Reprod Dev 1993;34:391-395
34. Hynes AC. Kane MT: Uptake and incorporation of myo-inositol by preimplantation cattle embryos. J Reprod Fertil 1994; 14:24 (abstr)
35. Nakanishi T, Burg MB: Osmoregulatory fluxes of myo-inositol and betaine in renal cells. Am J Physiol 1989;257:C964-C970
36. Gregoire AT, Gongsakdi D, Rakoff AE: The presence of inositol in genital tract secretions of the female rabbit. Fertil Steril 1962;13:432-435
37. Seidel GE Jr. Nauta W, Olson SE: Effects of myoinositol, transferrm, and insulin on culture of bovine embryos. J Ani Sci 1991;69 (Suppl 1):403 (abstr)
38. Carney EW, Foote RH: Improved development of rabbit one-cell embryos to the hatching blastocyst stage by culture in a defined protein-free culture medium. J Reprod Fertil 1991; 91:113-123