Application of a 5-bromo-2′-deoxyuridine ELISA for measuring the lymphoproliferative response to human cytomegalovirus in HIV-1-infected patients

Journal of Virological Methods

Volumn 105, Issue 2, 2002, Pages 247-251

  Tamarit, Amparo ^a   Alberola, Juan ^a   Galindo, María José ^b   Navarro, David ^a,b  

^a UNIVERSITY OF VALENCIA   (Spain)

^b HOSPITAL CLÍNICO UNIVERSITARIO   (Spain)

Author keywords

5 Bromo 2 deoxyuridine ELISA; HIV 1; Human cytomegalovirus; Lymphoproliferative response

Indexed keywords

BROXURIDINE;

ANALYTIC METHOD; ARTICLE; BLOOD CELL; CELL STIMULATION; CONTROLLED STUDY; CYTOMEGALOVIRUS; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; LABORATORY TEST; LYMPHOCYTE PROLIFERATION; MONITORING; MONONUCLEAR CELL; NONHUMAN; NORMAL HUMAN; PRIORITY JOURNAL; RADIOACTIVITY; T LYMPHOCYTE; TIME; VIRUS INACTIVATION; VIRUS INFECTION;

ACQUIRED IMMUNODEFICIENCY SYNDROME; AIDS-RELATED OPPORTUNISTIC INFECTIONS; BROMODEOXYURIDINE; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HIV-1; HUMANS; INDICATORS AND REAGENTS; LYMPHOCYTE ACTIVATION;

HUMAN HERPESVIRUS 5; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0036710641     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(02)00107-6     Document Type: Article

References (16)

1. + individuals. Clin. Exp. Immunol. 82:1991;556-559.
2. Hsieh S., Pan S., Hung C., Tsai H., Chen M., Chang S. Association between cytomegalovirus-specific reactivity of T-cell subsets and development of cytomegalovirus retinitis in patients with acquired immunodeficiency syndrome. J. Infect. Dis. 184:2001;1386-1391.
3. Huong P.L.T., Kolk A.H.J., Eggelte T.A., Verstinjen C.P.H.J., Gilis H., Hendriks J.T. Measurement of antigen specific lymphocyte proliferation using 5-bromo-deoxyuridine incorporation. J. Immunol. Methods. 140:1991;243-248.
4. + T lymphocyte immune function in long-term survivors of AIDS-related CMV end-organ disease who are receiving potent antiretroviral therapy. J. Infect. Dis. 183:2001;1399-1404.
5. + T-lymphocyte responses after ganciclovir and highly active antiretroviral therapy in individuals infected with HIV-1. Nat. Med. 4:1998;953-956.
6. + T-cell counts and recurrent retinitis. J. Infect. Dis. 183:2001;1285-1289.
7. + T-cell proliferation: comparison to 5-bromo-2′-deoxyuridine (BrdU) ELISA. J. Immunol. Methods. 223:1999;185-194.
8. Martinon F., Rabian C., Loiseau P., Ternynck T., Avrameas S., Colombani J. In vitro proliferation of human lymphocytes measured by an enzyme immunoassay using 5-bromo-2′-deoxyuridine monoclonal antibody. J. Clin. Lab. Immunol. 23:1987;153-158.
9. 3H] thymidine incorporation assay. Clin. Diagn. Lab. Immunol. 7:2000;687-692.
10. Porstmann T., Ternyck T., Avrameas S. Quantitation of 5-bromo-2′-deoxyuridine incorporation into DNA: an enzyme immunoassay for the assessment of the lymphoid cell proliferative response. J. Immunol. Methods. 82:1985;169-179.
11. Porstmann T., Ternyck T., Avrameas S. Quantitation of 5-bromo-2′-deoxyuridine incorporation into DNA: an enzyme immunoassay for the assessment of the lymphoid cell proliferative response. J. Immunol. Methods. 82:1985;169-179.
12. Schrier R.D., Freeman W.R., Wiley C.A., McCutchan J.A. Immune predispositions for cytomegalovirus retinitis in AIDS. J. Clin. Invest. 95:1995;1741-1746.
13. Wahren B., Morfeldt-Mansson L., Biberfeld L., Moburg A., Sonnerberg P., Ljungman P., Werner A., Kurth R., Gallo R., Bolognesi D. Characteristics of the specific cell-mediated immune response in human immunodeficiency virus infection. J. Virol. 61:1987;2017-2023.
14. + T cell frequencies by flow cytometry: evidence for a novel, antigen-specific homeostatic mechanism in HIV-associated immunodeficiency. J. Clin. Invest. 99:1997;1739-1750.
15. Weinberg A., Wohl D.A., Barrett J., Van der Host C. Inconsistent reconstitution of cytomegalovirus-specific cell-mediated immunity in human immunodeficiency virus-infected patients receiving highly active antiretroviral therapy. J. Infect. Dis. 184:2001;707-712.
16. Zanghellini F., Boppanna S.B., Emery V.C., Griffiths P.D., Pass R.F. Asymptomatic primary cytomegalovirus infection: virologic and immunologic features. J. Infect. Dis. 180:1999;702-707.