Application of molecular biological methods for studying probiotics and the gut flora

British Journal of Nutrition

Volumn 88, Issue SUPPL. 1, 2002, Pages

  McCartney, A L ^a  

^a UNIVERSITY OF READING   (United Kingdom)

Author keywords

Gut flora; Molecular methods; Probiotics

Indexed keywords

PROBIOTIC AGENT;

BACTERIUM; COMMENSALISM; CONFERENCE PAPER; DENATURING GRADIENT GEL ELECTROPHORESIS; DIETARY INTAKE; ECOSYSTEM; FLUORESCENCE IN SITU HYBRIDIZATION; GEL ELECTROPHORESIS; GENE SEQUENCE; GENETIC ANALYSIS; IN VIVO STUDY; INTESTINE FLORA; MICROBIAL DIVERSITY; MOLECULAR BIOLOGY; MONITORING; NONHUMAN; OLIGONUCLEOTIDE PROBE; ORGANISM COLONY; QUANTITATIVE ANALYSIS; RESEARCH; SEQUENCE ANALYSIS; TECHNOLOGY;

BACTERIA (MICROORGANISMS); MICROBIOTA;

EID: 0036709739     PISSN: 00071145     EISSN: None     Source Type: Journal    
DOI: 10.1079/bjn2002627     Document Type: Conference Paper

References (37)

1. Brigidi P, Vitali B, Swennen E, Altomare L, Rossi M & Matteuzzi D (2000) Specific detection of Bifidobacterium strains in a pharmaceutical probiotic product and in human faeces by PCR. Systematic and Applied Microbiology 23, 391-399.
2. Charteris WP, Kelly PM, Morelli L & Collins JK (1997) Review article: selective detection, enumeration and identification of potential probiotic Lactobacillus and Bifidobacterium species in mixed bacterial populations. International Journal of Food Microbiology 35, 1-27.
3. Franks AH, Harmsen HJM, Raangs GC, Jansen GJ, Schut F & Welling GW (1998) Variations of bacterial populations in human faeces measured by fluorescent in situ hybridisation with group-specific 16S rRNA-targeted oligonucleotide probes. Applied and Environmental Microbiology 64, 3336-3345.
4. Giraffa G & Neviani E (2000) Molecular identification and characterisation of food-associated lactobacilli. Italian Journal of Food Science 4, 403-423.
5. Hozapfel WH, Haberer P, Geisen R, Bjorkroth J & Schillinger U (2001) Taxonomy and important features of probiotic microorganisms in food and nutrition. American Journal of Clinical Nutrition 73(S), 365S-373S.
6. Jian W, Zhu L & Dong X (2001) New approach to phylogenetic analysis of the genus Bifidobacterium based on partial HSP60 gene sequences. International Journal of Systematic and Evolutionary Microbiology 51, 1622-1638.
7. Kimura K, McCartney AL, McConnell MA & Tannock GW (1997) Analysis of fecal populations of bifidobacteria and lactobacilli and investigation of the immunological responses of their human hosts to the predominant strains. Applied and Environmental Microbiology 63, 3394-3398.
8. Klein G, Pack A, Bonaparte C & Reuter G (1998) Taxonomy and physiology of probiotic lactic acid bacteria. International Journal of Food Microbiology 41, 103-125.
9. Kullen MJ, Brady LJ & O'Sullivan DJ (1997) Evaluation of using a short region of the recA gene for rapid and sensitive speciation of dominant bifidobacteria in the human large intestine. FEMS Microbiology Letters 154, 377-383.
10. Langendijk PS, Schut F, Jansen GJ, Raangs GC, Kamphuis GR, Wilkinson MHF & Welling GW (1995) Quantitative fluorescence in situ hybridisation of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples. Applied and Environmental Microbiology 61, 3069-3075.
11. Lebond-Bourget N, Philippe H, Mangin I & Decaris B (1996) 16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reveal inter- and intraspecific Bifidobacterium phylogeny. International Journal of Systematic Bacteriology 46, 102-111.
12. Lucchinin F, Kmet V, Cesena C, Coppi L, Bottazzi V & Morelli L (1998) Specific detection of a probiotic Lactobacillus strain in faecal samples by using multiplex PCR. FEMS Microbiology Letters 158, 273-278.
13. Marsh TL (1999) Terminal restriction fragment length polymorphism (T-RFLP): an emerging method for characterising diversity among homologous populations of amplification products. Current Opinions in Microbiology 2, 323-324.
14. Matsuki T, Watanabe K, Tanaka R, Fukuda M & Oyaizu H (1999) Distribution of bifidobacterial species in human intestinal microflora examined with 16S rRNA-gene-targeted species-specific primers. Applied and Environmental Microbiology 65, 4506-4512.
15. McCartney AL, Wang W & Tannock GW (1996) Molecular analysis of the composition of the bifidobacterial and lactobacillus microflora of humans. Applied and Environmental Microbiology 62, 4608-4613.
16. Muyzer G (1999) DGGE/TGGE a method for identifying genes from natural ecosystems. Current Opinions in Microbiology 2, 317-322.
17. Muyzer G, de Waal EC & Uitterlinden AG (1993) Profiling of complex microbial populations by DGGE analysis of PCR-amplified genes coding for 16S rRNA. Applied and Environmental Microbiology 59, 695-700.
18. O'Sullivan DJ (1999) Methods for analysis of the intestinal microflora. In Probiotics. A Critical Review, pp. 23-44 [GW Tannock, editor]. Norfolk. UK: Horizon Scientific Press.
19. O'Sullivan DJ & Kullen MJ (1998) Tracking of probiotic bifidobacteria in the intestine. International Dairy Journal 8, 513-525.
20. Prassad J, Gill H, Smart J & Gopal PK (1998) Selection and characterisation of Lactobacillus and Bifidobacterium strains for use as probiotics. International Dairy Journal 8, 993-1002.
21. Qiu X, Wu L, Huang H, McDonel PE, Palumbo AV, Tiedje JM & Zhou J (2001) Evaluation of PCR-generated chimeras, mutations, and heteroduplexes with 16S rRNA gene-based cloning. Applied and Environmental Microbiology 67, 880-887.
22. Roy D & Sirois S (2000) Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of the ldh gene. FEMS Microbiology Letters 191, 17-24.
23. Roy D, Sirois S & Vincent D (2001) Molecular discrimination of lactobacilli used as starter and probiotic cultures by amplified ribosomal DNA restriction analysis. Current Microbiology 42, 282-289.
24. Satokari RM, Vaughan EE, Akkermans ADL, Saarela M & de Vos WM (2001) Bifidobacterial diversity in human faeces detected by genus-specific PCR and denaturing gradient gel electrophoresis. Applied and Environmental Microbiology 67, 504-513.
25. Spratt BG (1999) Multilocus sequence typing: molecular typing of bacterial pathogens in an era of rapid DNA sequencing and the Internet. Current Opinions in Microbiology 2, 312-316.
26. Suau A, Bonnet R, Sutren M, Godon J-J, Gibson GR, Collins MD & Dore J (1999) Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Applied and Environmental Microbiology 65, 4799-4807.
27. Tannock GW, Tilsala-Timisjarvi A, Rodtong S, Ng J, Munro K & Alatossav T (1999) Identification of Lactobacillus isolates from the gastrointestinal tract, silage, and yoghurt by 16S-23S rRNA gene intergenic spacer region sequence comparisons. Applied and Environmental Microbiology 65, 4264-4267.
28. Tilsala-Timisjarvi A & Alatossava T (1997) Development of oligonucleotide primers from the 16S-23S rRNA intergenic sequences for identifying different dairy and probiotic lactic acid bacteria by PCR. International Journal of Food Microbiology 35, 49-56.
29. Tilsala-Timisjarvi A & Alatossava T (1998) Strain-specific identification of probiotic Lactobacillus rhamnosus with randomly amplified polymorphic DNA-derived PCR primers. Applied and Environmental Microbiology 64, 4816-4819.
30. Torriani S, Zapparoli G & Dellaglio F (1999) Use of PCR-based methods for rapid differentiation of L. delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis. Applied and Environmental Microbiology 65, 4351-4356.
31. Tynkkynen S, Satokari R, Saarela M, Mattila-Sandholm & Saxelin M (1999) Comparison of ribotyping, randomly amplified polymorphic DNA analysis, and pulsed-field gel electrophoresis in typing of Lactobacillus rhamnosus and L. casei strains. Applied and Environmental Microbiology 65, 3908-3914.
32. Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M & Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23, 4407-4414.
33. Walter J, Tannock GW, Tilsala-Timisjarvi A, Rodtong S, Loach DM, Munro K & Alatossava T (2000) Detection and identification of gastrointestinal Lactobacillus species by DGGE and species-specific PCR primers. Applied and Environmental Microbiology 66, 297-303.
34. Wang R-F, Cao W-W & Cerniglia CE (1996) PCR detection and quantification of predominant anaerobic bacteria in human and animal faecal samples. Applied and Environmental Microbiology 62, 1242-1247.
35. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP & Truper HG (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. International Journal of Systematic Bacteriology 37, 463-464.
36. Wintzingerode FV, Gobel UB & Stackebrandt E (1997) Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis. FEMS Microbiology Reviews 21, 213-229.
37. Zoetendal EG, Akkermans ADL & de Vos WM (1998) Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Applied and Environmental Microbiology 64, 3854-3859.