Rapid transbilayer movement of the fluorescent sterol dehydroergosterol in lipid membranes

Biophysical Journal

Volumn 83, Issue 3, 2002, Pages 1525-1534

  John, Karin ^a,b   Kubelt, Janek ^a,b   Müller, Peter ^a,b   Wüstner, Daniel ^a,b   Herrmann, Andreas ^a,b,c  

^a HUMBOLDT UNIVERSITY   (Germany)

^b MAX PLANCK INSTITUT FÜR PHYSIK KOMPLEXER SYSTEME   (Germany)

^c WEILL CORNELL MEDICAL COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 OLEOYL 1 PALMITOYLPHOSPHATIDYLCHOLINE; DEHYDROERGOSTEROL; LIPOSOME; METHYL BETA CYCLODEXTRIN; PHOSPHATIDYLETHANOLAMINE; PHOSPHORYLCHOLINE; STEROL DERIVATIVE; UNCLASSIFIED DRUG; BETA CYCLODEXTRIN DERIVATIVE; CYCLODEXTRIN; DRUG DERIVATIVE; ERGOSTEROL; LIPID; METHYL-BETA-CYCLODEXTRIN; STEROL;

ARTICLE; BILAYER MEMBRANE; ENERGY TRANSFER; LIPID MEMBRANE; LIQUID CRYSTAL; MEMBRANE TRANSPORT; PHOSPHOLIPID MEMBRANE; BIOPHYSICS; CHEMISTRY; FLUORESCENCE RESONANCE ENERGY TRANSFER; KINETICS; LIPID BILAYER; SPECTROFLUOROMETRY; SPECTROPHOTOMETRY; TEMPERATURE; THEORETICAL MODEL;

BETA-CYCLODEXTRINS; BIOPHYSICS; CYCLODEXTRINS; ERGOSTEROL; FLUORESCENCE RESONANCE ENERGY TRANSFER; KINETICS; LIPID BILAYERS; LIPIDS; LIPOSOMES; MODELS, THEORETICAL; PHOSPHORYLCHOLINE; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY; STEROLS; TEMPERATURE;

EID: 0036708461     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(02)73922-2     Document Type: Article

References (32)

1. The rapid transmembrane movement of cholesterol in small unilamellar vesicles
2. Transmembrane movement in small unilamellar vesicles detected by cholesterol oxidase
3. Transbilayer movement of cholesterol in the human erythrocyte membrane
4. Sphingolipid organization in biomembranes: What physical studies of model membranes reveal
5. Structure and origin of ordered lipid domains in biological membranes
6. Insolubility of lipids in Triton X-100: Physical origin and relationship to sphingolipid/cholesterol membrane domains (rafts)
7. Fluorescence studies of dehydroergosterol in phosphatidylethanolamine/phosphatidylcholine bilayers
8. Lipid rafts reconstituted in model membranes
9. Asymmetric transbilayer distribution of sterol across plasma membranes determined by fluorescence quenching of dehydroergosterol
10. Evidence for transbilayer, tail-to-tail cholesterol dimers in dipalmitoylglycerophosphocholine liposomes
11. Efflux of cholesterol from different cellular pools
12. Rates of spontaneous exchange of radiolabeled sterols between lipid vesicles
13. Saturated phospholipids with high melting temperatures form complexes with cholesterol in monolayers
14. Cellular cholesterol efflux mediated by cyclodextrins
15. The rate of transmembrane movement of cholesterol in the human erythrocyte
16. The Tangier disease gene product ABC1 controls the cellular apolipoprotein-mediated lipid removal pathway
17. Use of cyclodextrins to monitor transbilayer movement and different affinities of cholesterol
18. Dehydroergosterol structural organization in aqueous medium and in a model system of membranes
19. Rapid flip-flop of phospholipids in endoplasmic reticulum membranes studied by a stopped-flow approach
20. Distribution and transport of cholesterol in Caenorhabditis elegans
21. Membrane organization at low cholesterol concentrations: A study using 7-nitrobenz-2-oxa-1,3-diazol-4-y1-labeled cholesterol
22. Cholesterol distribution in living cells: Fluorescence imaging using dehydroergosterol as a fluorescent cholesterol analog
23. Cyclodextrin-mediated removal of sterols from monolayers: Effects of sterol structure and phospholipids on desorption rate
24. Cyclodextrincatalyzed extraction of fluorescent sterols from monolayer membranes and small unilamellar vesicles
25. Transbilayer movement of cholesterol in dipalmitoyllecithin-cholesterol vesicles
26. Transbilayer movement of cholesterol in phospholipid vesicles under equilibrium and non-equilibrium conditions
27. Transbilayer movement and net flux of cholesterol and cholesterol sulfate between liposomal membranes
28. Cholesterol organization in membranes at low concentrations: Effects of curvature stress and membrane thickness
29. Transmembrane distribution of sterol in the human erythrocyte
30. Functional rafts in cell membranes
31. A fluorescence energy transfer study of lecithin-cholesterol vesicles in the presence of phospholipase C
32. Cellular cholesterol efflux mediated by cyclodextrins