Structural characterisation of cysteines in a bacterial-binding motif of human salivary mucin MG2

Archives of Oral Biology

Volumn 47, Issue 8, 2002, Pages 591-597

  Soares, Rodrigo V ^a   Liu, Bing ^a   Oppenheim, Frank G ^a   Offner, Gwynneth D ^a   Troxler, Robert F ^a  

^a BOSTON UNIVERSITY   (United States)

Author keywords

Alkylation; Cysteine; MG2; Mucin; Saliva

Indexed keywords

AMINO ACID; CYSTEINE; DITHIOL DERIVATIVE; HUMAN SALIVARY MUCIN MG2; MUCIN; PROTEIN; PROTEIN MG2; PROTEINASE; SALIVA PROTEIN; UNCLASSIFIED DRUG;

ALKYLATION; ARTICLE; BACTERIUM; CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; ISOTOPE LABELING; MASS SPECTROMETRY; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN MOTIF; PROTEIN SECRETION; PROTEIN STRUCTURE; REDUCTION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SALIVARY GLAND; SUBLINGUAL GLAND; SUBMANDIBULAR GLAND;

AMINO ACID MOTIFS; AMINO ACIDS; BACTERIA; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYSTEINE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; MASS SPECTROMETRY; MUCINS; PROTEIN BINDING; SALIVARY PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 0036695330     PISSN: 00039969     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-9969(02)00047-X     Document Type: Article

References (26)

1. Biesbrock, A.R., Reddy, M.S., Levine, M.J., 1991. Interaction of a salivary mucin-secretory immunoglobulin A complex with mucosal pathogens. Infect. Immun. 59, 3492-3497.
2. Biesbrock, A.R., Bobek, L.A., Levine, M.J., 1997. MUC7 gene expression and genetic polymorphism. Glycoconjugate J. 14, 415-422.
3. Bobek, L.A., Tsai, H., Biesbrock, A.R., Levine, M.J., 1993. Molecular cloning, sequence, and specificity of expression of the gene encoding the low molecular weight human salivary mucin (MUC7). J. Biol. Chem. 268, 20563-20569.
4. Bobek, L.A., Liu, J., Salt, S.N., Shows, T.B., Bobek, Y.A., Levine, M.J., 1996. Structure and chromosomal localization of the human salivary mucin gene MUC7. Genomics 32, 277-282.
5. Bolscher, J.G.M., Groenink, J., van der Kwaak, J.S., van den Keijbus, P.A.M., van't Hof, W., Veerman, E.C.I., Nieuw Amerongen, A.V., 1999. Detection and quantification of MUC7 in submandibular, sublingual, palatine and labial saliva by anti-peptide antiserum. J. Dent. Res. 78, 1362-1369.
6. Carraway, K.L., Price-Schiavi, S.A., Komatsu, M, Idris, N., Perez, A., Li, P., Jepson, S., Zhu, X., Carvajal, M.E., Carraway, C.A., 2000. Multiple facets of sialomucin complex/MUC4, a membrane mucin and erbb2 ligand, in tumors and tissues. Frontiers Biosci. 5, 95-107.
7. Gibbons, R.J., Qureshi, J.V., 1978. Selective binding of blood group reactive salivary mucins by Streptococcus mutants and other organisms. Infect. Immun. 22, 665-671.
8. Groenink, J., Ligtenberg, A.J.M., Veerman, E.C.I., Bolsher, J.G.M., Nieuw Amerongen, A.V., 1996. Interaction of the salivary low-molecular-weight mucin (MG2) with Actinobacillus actinomycetemcomitans. Antonie van Leeuwenhoek 70, 79-87.
9. Gururaja, T.L., Ramasubbu, N., Venugopalan, P., Reddy, M.S., Ramalingam, K., Levine, M.J., 1998. Structural features of the human salivary mucin MUC7. Glycoconjugate J. 15, 457-467.
10. Iontcheva, I., Oppenheim, F.G., Troxler, R.F., 1997. Human: salivary mucin MG1 selectively forms heterotypic complexes with amylase, proline-rich proteins, statherin, and histatins. J. Dent. Res. 76, 734-743.
11. Jensen, J.L., Lamkin, M.S., Oppenheim, F.G., 1992. Adsorption of human salivary proteins to hydroxyapatite: a comparison between whole saliva and glandular salivary secretions. J. Dent. Res. 71, 1569-1576.
12. Levine, M.J., Herzberg, M.C., Levine, M.S., Ellison, S.A., Stinson, M.W., Li, H.C., van Dyke, T., 1978. Specificity of salivary-bacterial interactions: role of terminal sialic acid residues in the interaction of salivary glycoproteins with Streptococcus sanguis and Streptococcus mutans. Infect. Immun. 19, 107-115.
13. Ligtenberg, A.J.M., Walgreen-Weiteerings, E., Veerman, E.C.I., de Soet, J.J., de Graaff, J., Nieuw Amerongen, A.V., 1992. Influence of saliva on aggregation and adherence of Streptococcus gordonii HG222. Infect. Immun. 60, 3878-3884.
14. Liu, B., Rayment, S., Oppenheim, F.G., Troxler, R.F., 1999. Isolation of human salivary mucin MG2 by a novel method and characterization of its interactions with oral bacteria. Arch. Biochem. Biophys. 364, 286-293.
15. Liu, B., Rayment, S., Gyurko, C., Oppenheim, F.G., Offner, G.D., Troxler, R.F., 2000. The recombinant N-terminal region of human salivary mucin MG2 (MUC7) contains a binding domain for oral streptococci and exhibits candidacidal activity. Biochem. J. 345, 557-564.
16. Liu, B., Rayment, S.A., Soares, R.V., Oppenheim, F.G., Offner, G.D., Fives-Taylor, P., Troxler, R.F. Interaction of salivary mucin MG2, its recombinant N-terminal region and a synthetic peptide with Actinobacillus actinomycetemcomitans. J. Periodont. Res, in press.
17. Loomis, R.E., Prakobphol, A., Levine, M.J., Reddy, M.S., Jones, P.C., 1987. Biochemical and biophysical comparison of two mucins from human submandibular-sublingual saliva. Arch. Biochem. Biophys. 258, 452-464.
18. Mantle, M., Allen, A., 1978. A colorimetric assay for glycoproteins based on periodic acid/Schiff stain. Biochem. Soc. Trans. 6. 607-609.
19. Mehrotra, R., Thornton, D.J., Sheehan, J.K., 1998. Isolation and physical characterization of the MUC7 (MG2) mucin from saliva: evidence for self-association. Biochem. J. 334, 415-422.
20. Offner, G.D., Troxler, R.F., 2000. Heterogeneity of high-molecular-weight human salivary mucins. Adv. Dent. Res. 14, 69-75.
21. Perez-Villar, J., Hill, R.L., 1999. The structure and assembly of secreted mucins. J. Biol. Chem. 274, 31751-31754.
22. Rayment, S., Liu, B., Offner, G.D., Oppenheim, F.G., Troxler, R.F., 2000. Immunoquantification of human salivary mucins MG1 and MG2 in stimulated whole saliva: factors influencing mucin levels. J. Dent. Res. 79, 1765-1772.
23. Reddy, M.S., 1998. Binding of the pill of Pseudomonas aeruginosa to a low-molecular-weight mucin and neutral cystatin of human submandibular-sublingual saliva. Curr. Microbiol. 37, 395-402.
24. Sharma, P., Dudus, L., Nielsen, P.A., Clausen, H., Yankaskas, J.R., Hollingsworth, M.A., Engelhardt, J.F., 1998. MUC5B and MUC7 are differentially expressed in mucous and serous cells of submucosal glands in human bronchial airways. Am. J. Resp. Cell Mol. Biol. 19, 30-37.
25. Stinson, M.W., Levine, M.J., 1993. Modulation of intergeneric adhesion of oral bacterial by human saliva. Crit. Rev. Oral Biol. Med. 4, 309-314.
26. Tabak, L.A., Levine, M.J., Jain, N.K., Bryan, A.R., Cohen, R.E., Monte, L.D., Nancollas, G.H., Slomiany, A., Slomiany, B.L., 1985. Adsorption of human salivary mucins to hydroxyapatite. Arch. Oral Biol. 30, 423-427.