Conditional gene inactivation using cre recombinase

Calcified Tissue International

Volumn 71, Issue 2, 2002, Pages 100-102

  Schipani, E ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; CRE RECOMBINASE; DNA; MESSENGER RNA; MIFEPRISTONE; TAMOXIFEN; INTEGRASE; VIRUS PROTEIN;

ALLELISM; CONDITIONAL GENE TARGETING; CONTROLLED STUDY; DNA MODIFICATION; DNA RECOMBINATION; EMBRYO DEATH; ENZYME ANALYSIS; FERTILIZATION; FLOXXED GENE; GENE FUNCTION; GENE INACTIVATION; GENE LOCATION; GENE TARGETING; LACZ GENE; MOUSE; NONHUMAN; PRIORITY JOURNAL; REVIEW; SACCHAROMYCES CEREVISIAE; TISSUE SPECIFICITY; TRANSGENE; ANIMAL; GENE EXPRESSION REGULATION; GENE SILENCING; GENETICS; METHODOLOGY; MOUSE MUTANT;

ANIMALS; GENE SILENCING; GENE TARGETING; INTEGRASES; MICE; MICE, KNOCKOUT; MUTAGENESIS, INSERTIONAL; VIRAL PROTEINS;

EID: 0036691488     PISSN: 0171967X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00223-002-1004-y     Document Type: Review

References (16)

1. Orban P, Chui D, Marth J (1992) Tissue- and site-specific DNA recombination in transgenic mice. Proc Natl Acad Sci 89:6861-6865
2. Lakso M, Sauer B, Mosinger B, Lee EJ, Manning RW, Yu SH Mulder KL, Westphal H (1992) Targeted oncogene activation by site-specific recombination in transgenic mice. Proc Natl Acad Sci 89:6232-6236
3. Gu H, Marth J, Orban P, Mossmann H, Rajewsky K (1994) Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science 265:103-106
4. Sauer B (1998) Inducible gene targeting in mice using the Cre/lox system. Methods 14:381-392
5. Porter A (1998) Controlling your losses: conditional gene silencing in mammals. Trends Genet 14:73-79
6. Lewandoski M (2001) Conditional control of gene expression in the mouse. Nat Rev Gene 7443-7455
7. Wilson T, Kola I (2001) The LoxP/Cre system and genome modification. Methods Mol Biol 158:83-94
8. Le Y, Sauer B (2000) Conditional gene knockout using cre recombinase. Methods Mol Biol 136:477-485
9. Dymecki S (1996) Flp recombinase promotes site-specific DNA recombination in embryonic stem cells and transgenic mice. Proc Natl Acad Sci 93:6191-6196
10. Stark W, Boocock M, Sheratt D (1992) Catalysis by site-specific recombinases. Trends Genet 8:432-439
11. Duyne GV (2001) A structural view of cre-loxp site-specific recombination. Annu Rev Biophys Bionol Struct 30:87-104
12. Nagy A, Mar L (2001) Creation and use of a Cre recombinase transgenic database. Methods Mol Biol 158:95-106
13. Utomo A, Nikitin AY, Lee W (1999) Temporal, spatial and cell-type specific control of Cre-mediated DNA recombination in transgenic mice. Nature Biotech 17:1091-1096
14. Danielian P, Muccino D, Rowitch D, Michael S, McMahon A (1998) Modification of gene activity in mouse embryos in utero by a tamoxifen-inducible form of Cre recombinase. Curr Biol 8:1323-1326
15. Kellendonk C, Tronche F, Monaghan A, Angrand P, Stewart F, Schutz G (1996) Regulation of Cre recombinase activity by the synthetic steroid RU 486. Nucleic Acid Res 24:1404-1411
16. Soriano P (1999) Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet 21:70-71