Microdissection and sequence analysis of pericentric heterochromatin from the Drosophila melanogaster mutant Suppressor of Underreplication

Chromosoma

Volumn 111, Issue 2, 2002, Pages 114-125

  Moshkin, Yuri M ^a,b   Belyakin, Stephan N ^a   Rubtsov, Nikolay B ^a   Kokoza, Elena B ^a   Alekseyenko, Artem A ^a   Volkova, Elena I ^a   Belyaeva, Elena S ^a   Makunin, Igor V ^a,b   Spierer, Pierre ^b   Zhimulev, Igor F ^a  

^a INSTITUTE OF CYTOLOGY AND GENETICS   (Russian Federation)

^b UNIVERSITY OF GENEVA   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ARTICLE; CHROMOSOMAL LOCALIZATION; CHROMOSOME BAND; CHROMOSOME MAP; CHROMOSOME MICRODISSECTION; CHROMOSOME REPLICATION; CLONE; CONTROLLED STUDY; CYTOLOGY; DNA FLANKING REGION; DNA ISOLATION; DNA LIBRARY; DNA SEQUENCE; DROSOPHILA MELANOGASTER; EUCHROMATIN; GENE INSERTION; GENETIC STRAIN; GENOME; HETEROCHROMATIN; IN SITU HYBRIDIZATION; METAPHASE CHROMOSOME; MOLECULAR CLONING; MUTANT; NONHUMAN; POLYTENE CHROMOSOME; SEQUENCE ANALYSIS;

DROSOPHILA MELANOGASTER; MELANOGASTER;

EID: 0036664720     PISSN: 00095915     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00412-002-0190-8     Document Type: Article

References (28)

1. Adams MD, Celniker SE, Holt RA, Evans CA, Gocayne JD, et al. (2000) The genome sequence of Drosophila melanogaster. Science 287:2185-2195
2. Belyaeva ES, Zhimulev IF, Volkova EI, Alekseyenko AA, Moshkin YuM, et al. (1998) SuUR: A gene suppressing DNA underreplication in intercalary and pericentric heterochromatin of Drosophila melanogaster polytene chromosomes. Proc Natl Acad Sci U S A 95:7532-7537
3. Caizzi R, Caggese C, Pimpinelli S (1993) Bari-1, a new transposon-like family in Drosophila melanogaster with a unique heterochromatic organization. Genetics 133:335-345
4. Cryderman DE, Cuaycong MH, Elgin SC, Wallrath LL (1998) Characterization of sequences associated with position-effect variegation at pericentric sites in Drosophila heterochromatin. Chromosoma 107:277-285
5. Elgin SC (1996) Heterochromatin and gene regulation in Drosophila. Curr Opin Genet Dev 6:193-202
6. Gatti M, Pimpinelli S (1992) Functional elements in Drosophila melanogaster heterochromatin. Annu Rev Genet 26:239-275
7. Healy MJ, Russell RJ, Miklos GL (1988) Molecular studies on interspersed repetitive and unique sequences in the region of the complementation group uncoordinated on the X chromosome of Drosophila melanogaster. Mol Gen Genet 213:63-71
8. Heitz E (1933) Die somatische Heteropicnose bei Drosophila melanogaster und ihre genetische. Bedeutung Z Zellforsch 20:237-287
9. Karpen GH, Spradling AC (1992) Analysis of subtelomeric heterochromatin in the Drosophila minichromosome Dp1187 by single P element insertional mutagenesis. Genetics 132:737-753
10. Koryakov DE, Alekseyenko AA, Zhimulev IF (1999) Dynamic organization of the β-heterochromatin in the Drosophila melanogaster polytene X chromosome. Mol Gen Genet 260:503-509
11. Le MH, Duricka D, Karpen GH (1995) Islands of complex DNA are widespread in Drosophila centric heterochromatin. Genetics 141:283-303
12. Lohe AR, Hilliker AJ (1995) Return of the H-word (heterochromatin). Curr Opin Genet Dev 5:746-755
13. Makunin IV, Pokholkova GV, Kholodilov NG, Zakharkin SO, Bonaccorsi S, et al. (1999) A novel simple satellite DNA is co-localized with the Stalker retrotransposon in Drosophila melanogaster heterochromatin. Mol Gen Genet 261:381-387
14. Maleszka R, De Couet HG, Miklos GL (1998) Data transferability from model organisms to human beings: Insights from the functional genomics of the flightless region of Drosophila. Proc Natl Acad Sci U S A 95:3731-3736
15. Miklos GL, Yamamoto MT, Davies J, Pirrotta V (1988) Microcloning reveals a high frequency of repetitive sequences characteristic of chromosome 4 and the beta-heterochromatin of Drosophila melanogaster. Proc Natl Acad Sci USA 85:2051-2055
16. Mulder MP, Duijn P, Gloor HJ (1968) The replicative organization of DNA in polytene chromosomes of Drosophila hydei. Genetica 39:385-428
17. Rubtsov NB, Senger G, Kuzcera H, Neumann A, Kelbova C, et al. (1996) Interstitial deletion of chromosome 6q: Precise definition of the breakpoints by microdissection, DNA amplification, and reverse painting. Hum Genet 97:705-709
18. Semeshin VF, Belyaeva ES, Zhimulev IF (2001) Electron microscope mapping of pericentric and intercalary heterochromatin regions in polytene chromosomes of mutant Suppressor of underreplication in Drosophila melanogaster. Chromosoma 110:487-500
19. Spradling AC, Stern DM, Kiss I, Roote J, Laverty T, et al. (1995) Gene disruptions using P transposable elements: An integral component of the Drosophila genome project. Proc Natl Acad Sci U S A 92:10824-10830
20. Spradling AC, Stern D, Beaton A, Rhem EJ, Laverty T, et al. (1999) The Berkeley Drosophila genome project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics 153:135-177
21. Sun FL, Cuaycong MH, Craig CA, Wallrath LL, Locke J, et al. (2000) The fourth chromosome of Drosophila melanogaster: Interspersed euchromatic and heterochromatic domains. Proc Natl Acad Sci U S A 97:5340-5345
22. Sun X, Wahlstrom J, Karpen G (1997) Molecular structure of a functional Drosophila centromere. Cell 91:1007-1019
23. Tartof KD, Hobbs C, Jones M (1984) A structural basis for variegating position effects. Cell 37:869-878
24. Telenius H, Carter NP, Bebb CE, Nordenskjold M, Ponder BA, et al. (1992) Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primer. Genomics 13:718-725
25. Tudor M, Mitchelson A, O'Hare K (1996) A 1.5 kb repeat sequence flanks the suppressor of forked gene at the euchromatin-heterochromatin boundary of the Drosophila melanogaster X chromosome. Genet Res 68:191-202
26. Zhang P, Spradling AC (1994) Insertional mutagenesis of Drosophila heterochromatin with single P elements. Proc Natl Acad Sci USA 91:3539-3543
27. Zhang P, Spradling AC (1995) The Drosophila salivary gland chromocenter contains highly polytenized subdomains of mitotic heterochromatin. Genetics 139:659-670
28. Zhimulev IF (1998) Polytene chromosomes, heterochromatin, and position effect variegation. Adv Genet 37:1-566