Production of single chain recombinant monellin by high cell density culture of genetically engineered candida utilis using limited feeding of sodium ions

Journal of Chemical Engineering of Japan

Volumn 35, Issue 7, 2002, Pages 654-659

  Zhang, Xiaoli ^a,b   Ito, Takeshi ^b   Kondo, Keiji ^c   Kobayashi, Takeshi ^b   Honda, Hiroyuki ^b  

^a NORTHWEST UNIVERSITY   (China)

^b NAGOYA UNIVERSITY   (Japan)

^c KIRIN BREWERY CO LTD   (Japan)

Author keywords

Candida utilis; High cell density Culture; Jar Fermentor; Monellin; Sodium Ion

Indexed keywords

JAR FERMENTORS;

AMMONIA; GENETIC ENGINEERING; GLUCOSE; IONS; PHOSPHATES; PRODUCTION; SODIUM; TRACE ELEMENTS; YEAST;

CELL CULTURE;

PROTEIN;

EID: 0036637812     PISSN: 00219592     EISSN: None     Source Type: Journal    
DOI: 10.1252/jcej.35.654     Document Type: Article

References (9)

1. Almagro, A., C. Prista, S. Castro, C. Quintas, A. Madeira-Lopes, J. Ramos and M. C. Loureiro-Dias; "Effects of Salts on Debaryomyces hansenii and Saccharomyces cerevisiae under Stress Conditions," Int. J. Food Microbiol., 56, 191-197 (2000)
2. Eaton, A. D., L. S. Clesceri and A. E. Greensberg; "Phosphorus," Standard Methods for the Examination of Water and Wastewater, American Public Health Association, eds., 19th ed., pp. 106-115, Washington, D.C., USA (1995)
3. Juan, I. C., K. Janko, A. W. Ruud, P. van D. Johannes and T. P. Jack; "High-cell-density Cultivation of Yeasts on Disaccharides in Oxygen-limited Batch Cultures," Biotechnol. Bioeng., 46, 621-628 (1996)
4. Kim, S. H., C. H. Kang, R. Kim, J. M. Cho, Y. B. Lee and T. K. Lee; "Redesigning a Sweet Protein: Increased Stability and Renaturability," Protein Eng., 2, 571-575 (1989)
5. Kondo, K., T. Saito, S. Kajiwara, M. Takagi and N. Misawa; "A Transformation System for the Yeast Candida utilis: Use of a Modified Endogenous Ribosomal Protein Gene as a Drug-Resistant Marker and Ribosomal DNA as an Integration Target for Vector DNA," J. Bacteriol., 177, 7171-7177 (1995)
6. Kondo, K., Y. Miura, H. Sone, K. Kobayashi and H. Iijima; "High-level Expression of a Sweet Protein, Monellin, in the Food Yeast Candida utilis" Nat. Biotechnol., 15, 453-457 (1997)
7. Miura, Y., M. Kettoku, M. Kato, K. Kobayashi and K. Kondo; "High Level Production of Thermostable α-Amylase from Sulfolobus solfataricus in High-cell Density Culture of the Food Yeast Candida utilis" J. Mol. Microbiol. Biotechnol., 1, 129-134 (1999)
8. Morris, J. A. and R. H. Cagan; "Purification of Monellin, the Sweet Principle of Dioscoreophyllum cumminsii," Biochim. Biophys. Acta, 261, 114-122 (1972)
9. Patricia, E. T.-O., P. Antonio and R. Jorge; "Monovalent Cation Fluxes and Physiological Changes of Debaryomyces hansenii Grown at High Concentrations of KCl and NaCl," Yeast, 14, 1355-1371 (1998)