Molecular analysis of Escherichia coli O157 strains isolated from cattle and pigs by the use of PCR and pulsed-field gel electrophoresis methods

Veterinarni Medicina

Volumn 47, Issue 6, 2002, Pages 149-158

  Osek, Jacek ^a   Gallien, P ^b  

^a NATIONAL VETERINARY RESEARCH INSTITUTE   (Poland)

^b Federal Institute for Health Protection of Consumers and Veterinary Medicine   (Germany)

Author keywords

Cattle; E. coli O157; Genetic relatedness; Pigs; RFLP; Virulence marker genes

Indexed keywords

INTIMIN; VEROTOXIN;

ARTICLE; BACTERIAL VIRULENCE; CATTLE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE; GENETIC MARKER; NONHUMAN; PATHOGENICITY; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SWINE; TIR GENE;

EID: 0036614207     PISSN: 03758427     EISSN: None     Source Type: Journal    
DOI: 10.17221/5819-VETMED     Document Type: Article

References (41)

1. Adu-Bobie J., Frankel G., Bain C. Goncaleves A.G. Trabulsi L.R. Douce G., Knutton S., Dougan G. (1998): Detection of intimin α, β, γ, and δ, four intimin derivates expressed by attaching and effacing microbial pathogens. J. Clin. Microbiol., 36, 662-668
2. Ammon A., Peterson L.R., Karch H. (1999): A large outbreak of hemolytic uremic syndrome caused by an unusual sorbitol-fermenting strain E. coli O157: H-. J. Infect. Dis., 179, 1274-1277
3. Armstrong G.L., Hollingsworth J., Morris J.G. (1996): Emerging foodborne pathogens: Escherichia coli O157: H7 as a model of entry of a new pathogen into the food supply of the developed world. Epidemiol. Rev., 18, 29-51
4. Batchelor M., Knutton S., Caprioli A., Huter V., Zanial M., Dougan G., Frankel G. (1999): Development of a universal intimin antiserum and PCR primers. J. Clin. Microbiol., 32, 3822-3827
5. Beutin L., Montenegro M.A., Orskov I., Orskov F., Prada J., Zimmermann S., Stephan R. (1989): Close association of verotoxin (Shiga-like toxin) production with enterohemolysin production in strains of Escherichia coli. J. Clin. Microbiol., 27, 2559-2564
6. Beutin L., Geiger D., Steinruck H., Zimmermann S., Scheutz S. (1993): Prevalence and some properties of verotoxin (Shiga-like toxin)-producing Escherichia coli in seven different species of healthy domestic animals. J. Clin. Microbiol., 31, 2483-2488
7. Bielaszewska M., Schmidt H., Karmali M.A., Khakhria R., Janda J., Blahova K., Karch H. (1998): Isolation and characterization of sorbitol-fermenting Shiga toxin (Verocytotoxin)-producing Escherichia coli O157: H-strains in the Czech Republic. J. Clin. Microbiol., 36, 2135-2137
8. Bielaszewska M., Schmidt H., Liesegang A., Prager R., Rabsch W., Tschape H., Cizek A., Janda J., Blahova K., Karch H. (2000): Cattle can be a reservoir of sorbitol-fermenting Shiga toxin-producing Escherichia coli O157: H-strains and a source of human diseases. J. Clin. Microbiol., 38, 3470-3473
9. Bohm H., Karch H. (1992): DNA fingerprinting of Escherichia coli O157: H7 strains by pulsed-field gel electrophoresis. J. Clin. Microbiol., 30, 2169-2172
10. Cebula T.A., Payne W.I., Feng P. (1995): Simultaneous identification of strains of Escherichia coli serotype O157: H7 and their shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR. J. Clin. Microbiol., 33, 248-250
11. Chapman P.A., Siddons C.A., Malo A.T.C., Harkin M.A. (1997): A one-year study of Escherichia coli O157 in cattle, sheep, pigs and poultry. Epidemiol. Infect., 119, 245-250
12. Feng P., Lampel K.A., Karch H., Whittam T.S. (1998): Genotypic and phenotypic changes in the emergence of Escherichia coli O157: H7. J. Infect. Dis., 177, 1750-1753
13. Griffin P.M., Tauxe R.V. (1991): The epidemiology of infections caused by Escherichia coli O157: H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol. Rev., 13, 60-98
14. Heuvelink A.E., Zwartkruis-Nahuis J.T.M., Van Den Biggelaar F.L.A.M., Van Leeuwen W.J., De Boer E. (1999): Isolation and characterization of verocytotoxin-producing Escherichia coli O157 from slaughter pigs and poultry. Int. J. Food Microbiol., 52, 67-75
15. Imberechts H., De Greve H., Lintermans P. (1992): The pathogenesis of edema disease in swine. Vet. Microbiol., 31, 221-233
16. Jerse A.E., Yu J., Tall B.D., Kaper J.B. (1990): A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc. Natl. Acad. Sci. USA, 87, 7839-7843
17. Karch H., Bielaszewska M., Bitzan M., Schmidt H. (1999): Epidemiology and diagnosis of shiga toxin-producing Escherichia coli infections. Diagn. Microbiol. Infect. Dis., 34, 229-243
18. Kenny B., DeVinney R., Stein M., Reinscheid D.J., Frey E.A., Finlay B.B. (1997): Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells. Cell, 91, 511-520
19. Mainil J. (1999): Shiga/verocytotoxins and shiga/verotoxigenic Escherichia coli in animals. Vet. Res., 30, 235-257
20. Maurer J.J., Schmidt D., Petrosco P., Sanchez S., Bolton L., Lee M.D. (1999): Development of primers to O-antigen biosynthesis genes for specific detection of Escherichia coli O157 by PCR. Appl. Environ. Microbiol., 65, 2954-2960
21. McDaniel T.K., Jarvis K.G., Donnenberg M.S., Kaper J.B. (1995): A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc. Natl. Acad. Sci. USA, 92, 1664-1668
22. Meng J., Zhao S., Zhao T., Doyle M.P. (1995): Molecular characterisation of Escherichia coli O157: H7 isolates by pulsed-field gel electrophoresis and plasmid DNA analysis. J. Med. Microbiol., 42, 258-263
23. Nakazawa M., Akiba M., Sameshime T. (1999): Swine as a potential reservoir of Shiga toxin-producing Escherichia coli O157: H7 in Japan. Emmerg. Infect. Dis., 5, 833-834
24. O'Brien A.D., Holmes R. (1987): Shiga and Shiga-like toxins. Microbiol. Rev., 51, 206-220
25. Osek J. (1999a): Prevalence of shiga toxin genes among Escherichia coli strains isolated from pigs. Vet. Rec., 145, 557-558
26. Osek J. (1999b): Prevalence of virulence factors of Escherichia coli strains isolated from diarrheic and healthy piglets after weaning. Vet. Microbiol., 68, 209-217
27. Osek J. (2000): Clonal analysis of Escherichia coli strains isolated from pigs with post-weaning diarrhea by pulsed-field gel electrophoresis. FEMS Microbiol. Lett., 186, 327-331
28. Osek J. (2001): Molecular characterization of Shiga toxin-producing Escherichia coli O157 strains isolated in Poland. Res. Vet. Sci., 70, 175-177
29. Osek J. (2002): Identification of Escherichia coli O157: H7-strains from pigs with postweaning diarrhoea and PCR amplification of their virulence marker genes. Vet. Rec., in press
30. Osek J., Gallien P., Protz D. (2000): Characterization of shiga toxin-producing Escherichia coli strains isolated from calves in Poland. Comp. Immunol. Microbiol. Infect. Dis., 23, 267-276
31. Oswald E., Schmidt H., Morabito S., Karch H., Machers O., Caprioli A. (2000): Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: Characterization of a new intimin variant. Infect. Immun., 68, 64-71
32. Paton J.C., Paton A.W. (1998): Pathogenesis and diagnosis of shiga toxin-producing Escherichia coli infection. Clin. Microbiol. Rev., 11, 450-479
33. Paton A.W., Manning P.A., Woodrow M.C., Paton J.C. (1998): Translocated intimin receptors (Tir) of shiga-toxigenic Escherichia coli isolates belonging to serogroups O26, O111, and O157 react with sera from patients with hemolytic uremic syndrome and exhibit marked sequence heterogeneity. Infect. Immun., 66, 5580-5586
34. Pierard D., Muyldermans G., Moriau L., Stevens D., Lauvers S. (1998): Identification of new verotoxin type 2 variant B-subunit genes in human and animal Escherichia coli isolates. J. Clin. Microbiol., 36, 3317-3322
35. Schmidt H., Russmann H., Schwarzkopf A., Aleksic S., Heesemann J., Karch H. (1994): Prevalence of attaching and effacing Escherichia coli in stool samples from patients and controls. Zbl. Bakteriol., 281, 201-213
36. Schmidt H., Beutin L., Karch H. (1995): Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157: H7 strain EDL 933. Infect. Immun., 63, 1055-1061
37. Szych J., Paciorek J., Cieslik A., Kaluzewski S. (1998): Characteristics of E. coli O157 strains obtained from clinical materials and other sources in Poland (in Polish). Med. Dosw. Mikrobiol., 50, 179-196
38. Wang L., Rothemund D., Curd H., Reeves P.R. (2000): Sequence diversity of the Escherichia coli H7 fliC genes: Implication for a DNA-based typing scheme for E. coli O157: H7. J. Clin. Microbiol., 38, 1786-1790
39. Whittam T.S., Wachsmuth I.K., Wilson R.A. (1988): Genetic evidence of clonal descent of Escherichia coli O157: H7 associated with hemorrhagic colitis and hemolytic uremic syndrome. J. Infect. Dis., 157, 1124-1133
40. Wittig W., Klie H., Gallien P., Lehmann S., Timm M., Tschape H. (1995): Prevalence of the fimbrial antigens F18 and K88 and of the enterotoxins and verotoxins among Escherichia coli isolated from weaned pigs. Zbl. Bakteriol., 283, 95-104
41. Wray C., McLaren I.M., Carroll P.J. (1993): Escherichia coli isolated from farm animals in England and Wales between 1986 and 1991. Vet. Rec., 133, 439-442