Flexibility in monomeric Cu,Zn superoxide dismutase detected by limited proteolysis and molecular dynamics simulation

Proteins: Structure, Function and Genetics

Volumn 47, Issue 4, 2002, Pages 513-520

  Falconi, M ^a   Parrilli, L ^a   Battistoni, A ^a   Desideri, A ^a  

^a UNIVERSITY OF ROME TOR VERGATA   (Italy)

Author keywords

Cu,Zn superoxide dismutase; Limited proteolysis; Molecular dynamics; Protein cleavage; Protein dynamics; Protein fluctuation

Indexed keywords

ARGININE; COPPER ZINC SUPEROXIDE DISMUTASE; LYSINE; POLYPEPTIDE;

AMINO TERMINAL SEQUENCE; ARTICLE; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ESCHERICHIA COLI; GENE INSERTION; MEAN RESIDENCE TIME; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN LOCALIZATION; SIMULATION; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; ARGININE; COMPUTER SIMULATION; ESCHERICHIA COLI; KINETICS; LYSINE; MODELS, MOLECULAR; MOTION; SOLVENTS; SUPEROXIDE DISMUTASE; TRYPSIN; WATER;

ESCHERICHIA COLI; EUKARYOTA; INSERTION SEQUENCES;

EID: 0036606163     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/prot.10094     Document Type: Article

References (45)

1. Cu,Zn superoxide dismutase in prokaryotes and eukaryotes
2. Aspects of the structure, function, and applications of superoxide dismutase
3. Novel dimeric interface and electrostatic recognition in bacterial Cu,Zn superoxide dismutase
4. Cu,Zn superoxide dismutase from Photobacterium leiognathi is an hyperefficient enzyme
5. Evolutionary constraints for dimer formation in prokaryotic Cu,Zn superoxide dismutase
6. Unique structural features of the monomeric Cu,Zn superoxide dismutase from Escherichia coli, revealed by X-ray crystallography
7. Isolation of an active and heat-stable monomeric form of Cu,Zn superoxide dismutase from the periplasmic space of Escherichia coli
8. Role of the dimeric structure in Cu,Zn superoxide dismutase. pH-dependent, reversible denaturation of the monomeric enzyme from Escherichia coli
9. Protein measurement with the folin phenol reagent
10. Tricine-sodium dodecyl dulfatepolyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
11. The protein data bank
12. Atomic stress isobaric scaling for systems subjected to holonomic constraints
13. DL_POLY_2.0: A general-purpose parallel molecular dynamics simulation package
14. DLPROTEIN: A molecular dynamics package to simulate biomolecules
15. The missing term in effecttive pair potentials
16. Numerical integration of the Cartesian equations of motions of a system with constraints: Molecular dynamics of N-alkanes
17. A smooth particle mesh Ewald
18. Dictionary of protein secondary structure: Pattern recognition of hydrogen-bonded and geometrical features
19. Superposition of molecular structures using quaternions
20. The interpretation of protein structures: Estimation of static accessibility
21. Hydration and mobility of ions in solution
22. Computation of the mean residence time of water in the hydration shells of biomolecules
23. Solvent structure at a hydrophobic protein surface
24. Molecular dynamics simulation of solvated azurin: Influence of surface solvent accessibility on water residence times
25. Molecular dynamics simulations of Cu,Zn superoxide dismutase: Effect of temperature on dimer asymmetry
26. Dynamics-function correlation in Photobacterium leiognathi Cu,Zn superoxide dismutase: A spectroscopic and molecular dynamics simulation study
27. Water: Now you see it, now you don't
28. Residence times of water molecules in the hydration sites of myoglobin
29. Structural determinants of trypsin affinity and specificity for cationic inhibitors
30. Protein dynamics and conformational transitions in allosteric proteins
31. Identifying conformational changes with site-directed spin labeling
32. Measuring conformational dynamics of biomolecules by single molecule fluorescence spectroscopy
33. Protein dynamics from NMR
34. Protein dynamics from NMR
35. Probing the molten globule state of alpha-lactalbumin by limited proteolysis
36. Probing the conformational state of apomyoglobin by limited proteolysis
37. Probing the partly folded states of proteins by limited proteolysis
38. The structural aspects of limited proteolysis of native proteins
39. Correlation between sites of limited proteolysis and segmental mobility in thermolysin
40. Molecular dynamics simulations of human glutathione transferase P1-1: Conformational fluctuations of the apo-structure
41. The copper chaperone for superoxide dismutase
42. The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli
43. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures