Enhanced pepsin digestion: A novel process for purifying antibody F(ab′)2 fragments in high yield from serum

Journal of Immunological Methods

Volumn 263, Issue 1-2, 2002, Pages 57-74

  Jones, R G A ^a,c   Landon, J ^b  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^b Newcastle Emlyn   (United Kingdom)

^c ST BARTHOLOMEW S HOSPITAL   (United Kingdom)

Author keywords

Aggregation; Antivenom; Diafiltration; IgG; Pepsin; Sheep

Indexed keywords

ANTISERUM; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G; PEPSIN A; PIPERAZINE; TRYPSIN;

ARTICLE; CENTRIFUGATION; CONTAMINATION; CONTROLLED STUDY; DIAFILTRATION; DIGESTION; ENVENOMATION; GEL PERMEATION CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PRIORITY JOURNAL; PROTEIN AGGREGATION; PURIFICATION;

ANIMALS; BEE VENOMS; BEES; CHROMATOGRAPHY, ION EXCHANGE; ENZYME ACTIVATION; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN G; MOLECULAR WEIGHT; PEPSIN A; PRECIPITATION; SHEEP; SODIUM CHLORIDE; SOLUBILITY; TRYPSIN;

OVIS; OVIS ARIES; UNIDENTIFIED AGENTS;

EID: 0036571047     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00031-5     Document Type: Article

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