The morphogenic linker peptide of HBV capsid protein forms a mobile array on the interior surface

EMBO Journal

Volumn 21, Issue 5, 2002, Pages 876-884

  Watts, Norman R ^a   Conway, James F ^a   Cheng, Naiqian ^a  

^a National Cancer Intitute   (United States)

Author keywords

Core antigen; Cryo electron microscopy; Hepatitis B virus; Nucleic acid packaging; Nucleocapsid morphogenesis

Indexed keywords

CAPSID PROTEIN;

ARTICLE; CRYSTAL STRUCTURE; HEPATITIS B VIRUS; MORPHOGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; VIRUS CAPSID;

AMINO ACID SUBSTITUTION; CAPSID; CARBOHYDRATE DEHYDROGENASES; CHROMATOGRAPHY, GEL; CIRCULAR DICHROISM; CRYOELECTRON MICROSCOPY; CRYSTALLIZATION; ESCHERICHIA COLI; HEPATITIS B CORE ANTIGENS; HEPATITIS B E ANTIGENS; HEPATITIS B VIRUS; IMAGE PROCESSING, COMPUTER-ASSISTED; MODELS, MOLECULAR; MORPHOGENESIS; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; SUBTRACTION TECHNIQUE;

DNA VIRUSES; HEPATITIS B VIRUS;

EID: 0036500146     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/21.5.876     Document Type: Article

References (45)

1. A model-based approach for determining orientations of biological macromolecules imaged by cryoelectron microscopy
2. Hepadnaviral assembly is initiated by polymerase binding to the encapsidation signal in the viral RNA genome
3. Carboxy-terminal truncations of the HBV core protein affect capsid formation and the apparent size of encapsidated HBV RNA
4. Low-resolution density maps from atomic models: How stepping 'back' can be a step 'forward
5. Hepatitis B virus nucleocapsid assembly: Primary structure requirements in the core protein
6. Hepatitis B virus, the vaccine and the control of primary cancer of the liver
7. Determination of the fold of the core protein of hepatitis B virus by electron cryomicroscopy
8. Finding the small difference: A nine amino acid extension to the hepatitis B core protein
9. Folding and assembly of hepatitis B virus core protein: A new model proposal
10. Structure of small virus-like particles assembled from the L1 protein of human papillomavirus 16
11. Tetrairidium, a four-atom cluster, is readily visible as a density label in three-dimensional cryo-EM maps of proteins at 10-25 Å resolution
12. Electron microscopy of hepatitis B core antigen synthesized in E. Coli
13. Methods for reconstructing density maps of 'single particles' from cryoelectron micrographs to subnanometer resolution
14. Visualization of a 4-helix bundle in the hepatitis B virus capsid by cryo-electron microscopy
15. Hepatitis B virus capsid: Localization of the putative immunodominant loop (residues 78 to 83) on the capsid surface and implications for the distinction between c and e-antigens
16. Localization of the N terminus of hepatitis B virus capsid protein by peptide-based difference mapping from cryoelectron microscopy
17. Three-dimensional structure of hepatitis B virus core particles determined by electron cryomicroscopy
18. Three-dimensional reconstruction of icosahedral particles - The uncommon line
19. A recombinant hepatitis B core antigen polypeptide with the protamine-like domain deleted self-assembles into capsid particles but fails to bind nucleic acids
20. A new scaffold for binding haem in the cytochrome domain of the extracellular flavocytochrome cellobiose dehydrogenase
21. Image contrast in high-resolution electron microscopy of biological macromolecules: TMV in ice
22. Improved methods for building protein models in electron density maps and the location of errors in these models
23. Evolutionary conservation in the hepatitis B virus core structure: Comparison of human and duck cores
24. Poliovirus neutralization by antibodies to internal epitopes of VP4 and VP1 results from reversible exposure of these sequences at physiological temperature
25. Proline-138 is essential for the assembly of hepatitis B virus core protein
26. The humoral immune response in acute and chronic hepatitis B virus infection
27. Poles of partly unfolded conformations in macromolecular self-assembly
28. The arginine-rich domain of the hepatitis B virus core protein is required for pregenome encapsidation and productive viral positive-strand DNA synthesis but not for virus assembly
29. Improved tools for biological sequence comparison
30. HBc and HBe antigenicity and DNA-binding activity of major core protein P22 in hepatitis B virus core particles isolated from the cytoplasm of human liver cells
31. An RNA stem-loop structure directs hepatitis B virus genomic RNA encapsidation
32. Antigenic determinants and functional domains in core antigen and e antigen from hepatitis B virus
33. Direct cloning and sequence analysis of enzymically amplified genomic sequences
34. Hepatitis B virus core and e antigen: Immune recognition and use as a vaccine carrier moiety
35. Structure of a human rhinovirus-bivalently bound antibody complex: Implications for viral neutralization and antibody flexibility
36. Immunogenicity of peptide fusions to hepatitis B virus core antigen
37. Difference imaging of adenovirus: Bridging the resolution gap between X-ray crystallography and electron microscopy
38. Construction of mutant and chimeric genes using the polymerase chain reaction
39. Role of the N-terminal part of the coat protein in the assembly of cowpea chlorotic mottle virus. A 500 MHz proton nuclear magnetic resonance study and structural calculations
40. Hepatitis core antigen produced in Escherichia coli: Subunit composition, conformational analysis and in vitro capsid assembly
41. The crystal structure of the human hepatitis B virus capsid
42. Chimeric hepatitis B virus core particles with parts or copies of the hepatitis C virus core protein
43. Dimorphism of hepatitis B virus capsids is strongly influenced by the C-terminus of the capsid protein
44. Localization of the C terminus of the assembly domain of hepatitis B virus capsid protein: Implications for morphogenesis and organization of encapsidated RNA
45. A theoretical model successfully identifies features of hepatitis B virus capsid assembly