Glucose deprivation and hypoxia increase the expression of the GLUT1 glucose transporter via a specific mRNA cis-acting regulatory element

Journal of Neurochemistry

Volumn 80, Issue 3, 2002, Pages 552-554

  Boado, Ruben J ^a   Pardridge, William M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Blood brain barrier; Gene regulation; Glucose transporter; GLUT1; Hypoglycemia; Hypoxia

Indexed keywords

CIS ACTING ELEMENT; GLUCOSE; GLUCOSE TRANSPORTER 1; LUCIFERASE; MESSENGER RNA; NUCLEOTIDE; OXYGEN;

3' UNTRANSLATED REGION; ANIMAL CELL; ARTICLE; BLOOD BRAIN BARRIER; CONTROLLED STUDY; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; HYPOGLYCEMIA; HYPOXIA; NONHUMAN; PRIORITY JOURNAL; RAT; REPORTER GENE; RNA DEGRADATION; SITE DIRECTED MUTAGENESIS;

ANIMALS; BLOOD-BRAIN BARRIER; CELL HYPOXIA; CELL LINE; GENE EXPRESSION REGULATION; GLUCOSE; GLUCOSE TRANSPORTER TYPE 1; HYPOGLYCEMIA; HYPOXIA, BRAIN; MONOSACCHARIDE TRANSPORT PROTEINS; MUTAGENESIS, SITE-DIRECTED; OXYGEN; RATS; REGULATORY SEQUENCES, NUCLEIC ACID; RNA, MESSENGER;

ANIMALIA;

EID: 0036488221     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0022-3042.2001.00756.x     Document Type: Article

References (24)

1. Behrooz A. and Ismail-Beigi F. (1997) Dual control of glutl glucose transporter gene expression by hypoxia and by inhibition of oxidative phosphorylation. J. Biol. Chem. 272, 5555-5562.
2. Boado R. J. (1998) Brain-derived peptides increase blood-brain barrier GLUT1 glucose transporter gene expression via mRNA stabilization. Neurosci. Lett. 23, 147-150.
3. Boado R. J. (2000) Post-transcription modulation of the blood-brain barrier GLUT1 glucose transporter by brain-derived factors. J. Neural Transm. Suppl. 59, 255-261.
4. Boado R. J. and Pardridge W. M. (1993) Glucose deprivation causes post-transcriptional enhancement of brain capillary endothelial glucose transporter gene expression via GLUT1 mRNA stabilization. J. Neurochem. 60, 2290-2296.
5. Boado R. J. and Pardridge W. M. (1998) Ten nucleotide cis element in the 3′-untranslated region of the GLUT1 glucose transporter mRNA increases gene expression via mRNA stabilization. Mol. Brain Res. 15, 109-113.
6. Cornford E. M., Hyman S., Black K. L., Cornford M. E., Vinters H. V. and Pardridge W. M. (1995) High expression of Glut1 glucose transporter in human brain hemangioblastoma endothelium. J. Neuropath. Exp. Neuro. 54, 842-851.
7. Dwyer K. J., Boado R. J. and Pardridge W. M. (1996) Cis-element/cytoplasmic protein interaction within the 3′-untranslated region of the GLUT1 glucose transporter mRNA. J. Neurochem. 66, 449-458.
8. Hamilton B. J., Nichols R. C., Tsukamoto H., Boado R. J., Pardridge W. M. and Rigby W. F. (1999) hnRNP A2 and hnRNP L bind the 3′UTR of glucose transporter 1 mRNA and exist as a complex in vivo. Biochem. Biophys. Res. Commun. 261, 646-651.
9. Jain R. G., Andrews L. G., McGowan K. M., Pekala P. H. and Keene J. D. (1997) Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes. Mol. Cell. Bio. 17, 954-962.
10. Kumagai A. K., Kang Y.-S., Boado R. J. and Pardridge W. M. (1995) Upregulation of blood-brain barrier GLUT1 glucose transporter protein and mRNA in experimental chronic hypoglycemia. Diabetes 44, 1399-1404.
11. Lee W. H. and Bondy C. A. (1993) Ischemic injury induces brain glucose transporter gene expression. Endocrinology 133, 2540-2544.
12. Levy D. E. and Duffy T. E. (1977) Cerebral energy metabolism during transient ischemia and recovery in the gerbil. J. Neurochem. 28, 63-70.
13. Levy A. P., Levy N. S. and Goldberg M. A. (1996) Hypoxia-inducible protein binding to vascular endothelial growth factor mRNA and its modulation by the von Hippel-Lindau protein. J. Biol. Chem. 271, 25492-25497.
14. MacMillan V. and Siesjo B. K. (1972) Brain energy metabolism in hypoxemia. Scand. J. Clin. Lab. Invest. 30, 127-136.
15. Maher F. and Harrison L. C. (1990) Hexose specificity for downregulation of HepG2/brain-type glucose transporter gene expression in L6 myocytes. Diabetologia 33, 641-648.
16. McCall A. L., Fixman L. B., Fleming N., Tornheim K., Chick W. and Ruderman N. B. (1986) Chronic hypoglycemia increases brain glucose transport. Am. J. Physiol. 251, E442-E447.
17. McCall A. L., Van Bueren A. M., Nipper V., Moholt-Siebert M., Downes H. and Lessov N. (1996) Forebrain ischemia increases Glut1 protein in brain microvessels and parenchyma. J. Cereb. Blood Flow Metabol. 16, 69-76.
18. McGowan K. M., Police S., Winslow J. B. and Pekala P. H. (1997) Tumor necrosis factor-α regulation of glucose transporter (GLUT1) mRNA turnover: Contribution of the 3′-untranslated region of the GLUT1 message. J. Biol. Chem. 272, 1331-1337.
19. Pardridge W. M., Boado R. J. and Farrell C. R. (1990) Brain-type glucose transporter (GLUT-1) is selectively localized to the blood-brain barrier. Studies with quantitative Western blotting and in situ hybridization. J. Biol. Chem. 265, 18035-18040.
20. Stein I., Neeman M., Shweiki D., Itin A. and Keshet E. (1995) Stabilization of vascular endothelial growth factor mRNA by hypoxia and hypoglycemia and coregulation with other ischemia-induced genes. Mol. Cell. Biol. 15, 5363-5368.
21. Stephens J. M., Carter B. Z., Pekala P. H. and Malter J. S. (1992) Tumor necrosis factor α-induced glucose transporter (GLUT-1) mRNA stabilization in 3T3-L1 preadipocytes. J. Biol. Chem. 267, 8336-8341.
22. Tsukamoto H., Boado R. J. and Pardridge W. M. (1996) Differential expression in glioblastoma multiforme and cerebral hemangioblastoma of cytoplasmic proteins that bind two different domains within the 3′-untranslated regions of the human glucose transporter 1 (GLUT1) messenger RNA. J. Clin. Invest. 97, 2823-2832.
23. Tsukamoto H., Hamada Y., Wu D., Boado R. J. and Pardridge W. M. (1998) GLUT1 glucose transporter: differential gene transcription and mRNA binding to cytosolic and polysome proteins in brain and peripheral tissues. Mol. Brain Res. 58, 170-177.
24. Turman M. A. and Rosenfeld S. L. (1999) Heat shock protein 70 overexpression protects LLC-PK1 tubular cells from heat shock but not hypoxia. Kidney Int. 55, 189-197.