Administration of estradiol-3-benzoate down-regulates the expression of testicular steroidogenic enzyme genes for testosterone production in the adult rat

Journal of Veterinary Medical Science

Volumn 64, Issue 2, 2002, Pages 107-113

  Sakaue, Motoharu ^a,b   Ishimura, Ryuta ^b,c   Kurosawa, Shuichi ^b   Fukuzawa, Noriho H ^b,c   Kurohmaru, Masamichi ^a   Hayashi, Yoshihiro ^a   Tohyama, Chiharu ^b,c   Ohsako, Seiichiroh ^b,c  

^a UNIVERSITY OF TOKYO   (Japan)

^b NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

Estrogen; Rat; Steroidogenic enzyme; Testis; Testosterone

Indexed keywords

3 (OR 17) BETA HYDROXYSTEROID DEHYDROGENASE; 3 (OR 17)-BETA-HYDROXYSTEROID DEHYDROGENASE; CYTOCHROME P450; DRUG DERIVATIVE; ESTRADIOL; ESTRADIOL 3-BENZOATE; ESTRADIOL BENZOATE; HYDROXYSTEROID DEHYDROGENASE; LUTEINIZING HORMONE; TESTOSTERONE;

AGE; ANIMAL; ANIMAL DISEASE; ARTICLE; DOSE RESPONSE; DOWN REGULATION; DRUG EFFECT; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; MALE; METABOLISM; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SECRETION; SPRAGUE DAWLEY RAT; TESTIS;

17-HYDROXYSTEROID DEHYDROGENASES; AGE FACTORS; ANIMALS; CYTOCHROME P-450 ENZYME SYSTEM; DOSE-RESPONSE RELATIONSHIP, DRUG; DOWN-REGULATION; ESTRADIOL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; LUTEINIZING HORMONE; MALE; RATS; RATS, SPRAGUE-DAWLEY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TESTIS; TESTOSTERONE;

EID: 0036483532     PISSN: 09167250     EISSN: None     Source Type: Journal    
DOI: 10.1292/jvms.64.107     Document Type: Article

References (32)

1. Anderson, C. M. and Mendelson, C. R. 1985. Regulation of steroidogenesis in rat Leydig cells in culture. Arch. Biochem. Biophys. 238: 378-387.
2. Blanco-Rodriguez, J. and Martinez-Garcia, C. 1998. Apoptosis precedes detachment of germ cells from the seminiferous epithelium after hormone suppression by short-term oestradiol treatment of rats. Int. J. Andorol. 21: 109-115.
3. Brinkmann, A. O., Leemborg, F. G., Roodnat, E. M., de Jong, F. H. and van der Molen, H. J. 1980. A specific action of estradiol on enzymes involved in testicular steroidogenesis. Biol. Reprod. 23: 801-809.
4. Chomczynski, P. and Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156-159.
5. Eddy, E. M., Washburn, T. F., Bunich, D. O., Goulding, E. H., Gladen, B. C., Lubahn, D. B. and Korach, K. S. 1996. Targeted disruption of the estrogen receptor gene in male mice causes alteration of spermatogenesis and infertility. Endocrinology 137: 4796-4805.
6. Fisher, J. S., Millar, M. R., Majdic, G., Saunders, P. T. K., Fraser, H. M. and Sharpe, R. M. 1997. Immunolocalization of oestrogen receptor-alpha within the testis and excurrent ducts of the rat and marmoset monkey from perinatal life to adulthood. J. Endocrinol. 153: 485-495.
7. Givens, C. R., Zhang, P., Bair, S. R. and Mellon, S. H. 1994. Transcriptional regulation of rat cytochrome P450c17 expression in mouse Leydig MA-10 and adrenal Y-1 cells: identification of a single protein that mediates both basal and cAMP-induced activities. DNA Cell Biol. 13: 1087-1098.
8. Govoroun, M., Mcmeel, O. M., Mecherouki, H., Smith, T. J. and Guiguen, Y. 2001. 17β-Estradiol treatment decrease steroidogenic enzyme messenger ribonucleic acid levels in the rainbow trout testis. Endocrinology 142: 1841-1848.
9. Hess, R. A., Bunick, D., Lee, K., Bahr, J., Taylor, J. A., Korach, K. S. and Lubahn, D. B. 1997. A role for oestrogens in the male reproductive system. Nature (Lond.) 390: 509-512.
10. Jong, F. H., Uilenbroek, J. and Van der Molen, H. J. 1975. Oestradiol-17β, testosterone and gonadotrophins in oestradiol-17β treated intact adult male rats. J. Endocrinol. 65: 281-282.
11. Kremers, P., Tixhon, P. K. C. and Gielen, J. 1977. 17-alpha-hydroxylase and testosterone biosynthesis in rat testes. J. Steroid Biochem. 8: 873-877.
12. Lee, V. W. K., de Kretser, D. M., Hudson, B. and Wang, C. 1975. Variations in serum FSH, LH and testosterone levels in male rats from birth to sexual maturity. J. Reprod. Fertil. 42: 121-126.
13. Li, P. S. 1991. In vitro effects of estradiol, diethylstilbestrol and tamoxifen on testosterone production by purified pig Leydig cells. Chinese J. Physiol. 34: 287-301.
14. McGarvey, C., Cates, P. A., Brooks. A., Swanson, I. A., Milligan, S. R., Coen, C. W. and O'Byrne, K. T. 2001. Phytoestrogens and gonadotropin-releasing hormone pulse generator activity and pituitary luteinizing hormone release in the rat. Endocrinology 142: 1202-1208.
15. Melner, M. H. and Abney, T. O. 1980. The direct effect of 17 beta-estradiol on LH-stimulated testosterone production in hypophysectomized rats. J. Steroid Biochem. 13: 203-210.
16. Molenaar, R., Rommerts, F. F. and van der Molen, H.J. 1983. The steroidogenic activity of isolated Leydig cells from mature rats depends on the isolation procedure. Int. J. Androl. 6: 261-274.
17. Nakajin, S. and Hall, P. F. 1981. Microsomal cytochrome P-450 from neonatal pig testis: purification and properties of a C21 steroid side chain cleavage system (17α-hydroxylase and C17.20 lyase). J. Biol. Chem. 256: 3871-3787.
18. Namiki, M., Kitamura, M., Nonomura, N., Sugao, H., Nakamura, M, Okuyama, A., Utsunomiya, M., Itatani, H., Matsumoto, K. and Sonoda, T. 1988. Direct inhibitory effect of estrogen on the human testis in vitro. Arch. Androl. 20: 131-135.
19. Ohba, H., Inano, H. and Tamaoki, B. 1982. Kinetic mechanism of porcine testicular 17β-hydroxysteroid dehydrogenase. J. Steroid Biochem. 17: 381-389.
20. Oonk, R. B., Krasnow, J. S., Beattie, W. G. and Richards, J. S. 1989. Cyclic AMP-dependent and -independent regulation of cholesterol side chain cleavage cytochrome P-450 (P-450scc) in rat ovarian granulosa cells and corpora lutea. cDNA and deduced amino acid sequence of rat P-450scc. J. Biol. Chem. 264: 21934-21942.
21. Oshima, H. and Ochiai, K. 1973. On testicular 17β-hydroxysteroid oxidoreductase product activation of testosterone formation from androstenedione in vitro. Biochim. Biophys. Acta. 306: 227-235.
22. Payne, A. H. and O'Shaughnessy, P. J. 1996. Structure, function and regulation of steroidogenic enzymes in the Leydig cell. pp. 259-268. In: The Leydig Cell (Payne, A. N., Hardy, M. P. and Russell, L. D. eds.), Cashe River Press, Vienna, IL.
23. Pierantoni, R., Varriale, B., Minucci, S., Di Matteo, L., Fasano, S., D'Antonio, M. and Chieffe, G. 1986. Regulation of androgen production by frog (Rana esculenta) testis: an in vitro study on the effects exerted by estradiol, 5α-dihydrotestosterone, testosterone, melatonin, and serotonin. Gen. Comp. Endocrinol. 64: 405-410.
24. Robertson, K. M., O'Donnell, L., Jones, M. E. E., Meachem, S. J., Boon, W. C., Fisher, C. R., Graves, K. H., McLachlan, R. I. and Simpson, E. R. 1999. Impairment of spermatogenesis in mice lacking a functional aromatase (cyp 19) gene. Proc. Natl. Acad. Sci. U. S. A. 96: 7986-7991.
25. Shikita, M. and Hall, P. F. 1973. Cytochrome P-450 from bovine adrenocortical mitochondria: an enzyme for the side-chain cleavage of cholesterol. I. Purification and properties. J. Biol. Chem. 248: 5598-5606.
26. Steinberger, E. and Duckett, G. E. 1967. Hormonal control of spermatogenesis. J. Reprod. Fertil. (Suppl.) 2: 75-87.
27. Steinberger, E., Chowdhury, M. and Tcolakian, R. K. 1977. Effect of estradiol benzoate on the pituitary-gonadal axis in the intact male rat. Andrologia 9: 307-312.
28. Tang, P., Tsai-Morris, C. H. and Dufau, M. L. 1998. Regulation of 3β-hydroxysteroid dehydrogenase in gonadotropin-induced steroidogenic desensitization of Leydig cells. Endocrinology 139: 4496-4505.
29. Tcholakian, R. K., Chowdhury, M. and Steinberger, E. 1974. Time of action of oestradiol-17beta on luteinizing hormone and testosterone. J. Endocrinol. 63: 411-412.
30. Tsai-Morris, C. H., Khanum, A., Tang, P. and Dufau, M. L. 1999. The rat 17β-hydroxysteroid dehydrogenase type III: molecular cloning and gonadotropin regulation. Endocrinology 140: 3534-3542.
31. Zanato, V. F., Martins, M. P., Anselmo-Franci, J. A., Petenusci, S. O. and Lamano-Carvalho, T. L. 1994. Sexual development of male Wistar rats. Braz. J. Med. Biol. Res. 27: 1273-1280.
32. van Pelt, ANS M. M., de Rooij, D. G., van der Burg, B., van der Saag, P. T., Gustafsson, J. and Kuiper, G. G. J. M. 1999. Ontogeny of estrogen receptor-beta expression in rat testis. Endocrinology 140: 478-483.