Expression and refolding of recombinant human α-tocopherol transfer protein capable of specific α-tocopherol binding

Protein Expression and Purification

Volumn 24, Issue 3, 2002, Pages 395-403

  Panagabko, Candace ^a   Morley, Samantha ^b   Neely, Steve ^a   Lei, Huangshu ^c   Manor, Danny ^b   Atkinson, Jeffrey ^a  

^a BROCK UNIVERSITY   (Canada)

^b Department of Obstetrics Gynecology   (United States)

^c PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI; MAMMALIA;

EID: 0036451716     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1576     Document Type: Article

References (35)

1. Burton, G. W., and Traber, M. G. (1990) Vitamin E: Antioxidant activity, biokinetics, and bioavailability. Annu. Rev. Nutr. 10, 357-382.
2. Traber, M., and Sies, H. (1996) Vitamin E in humans: Demand and delivery. Annu. Rev. Nutr. 16, 321-347.
3. Brigelius, F.-R., and Traber, M. G. (1999) Vitamin E: Function and metabolism. FASEB J. 13(10), 1145-1155.
4. Traber, M. G., and Arai, H. (1999) Molecular mechanisms of vitamin E transport. Annu. Rev. Nutr. 19, 343-355.
5. Arita, M., et al. (1995) Human alpha-tocopherol transfer protein: cDNA cloning, expression and chromosomal localization. Biochem. J. 306, 437-443.
6. Hosomi, A., et al. (1998) Localization of alpha-tocopherol transfer protein in rat brain. Neurosci. Lett. 256(3), 159-162.
7. Copp, R. P., et al. (1999) Localization of alpha-tocopherol transfer protein in the brains of patients with ataxia with vitamin E deficiency and other oxidative stress related neurodegenerative disorders. Brain Res. 822(1/2), 80-87.
8. Sha, B., et al. (1998) Crystal structure of the Saccharomyces cerevisiae phosphatidylinositol transfer protein. Nature 391, 506-510.
9. Zimmer, S., et al. (2000) A novel human tocopherol-associated protein - Cloning, in vitro expression, and characterization. J. Biol. Chem. 275(33), 25672-25680.
10. Stocker, A., et al. (1999) Identification of a novel cytosolic tocopherol-binding protein: Structure, specificity, and tissue distribution. IUBMB Life 48(1), 49-56.
11. Shibata, N., et al. (2001) Supernatant protein factor, which stimulates the conversion of squalene to lanosterol, is a cytosolic squalene transfer protein and enhances cholesterol biosynthesis. Proc. Natl. Acad. Sci. USA 98(5), 2244-2249.
12. Dutta-Roy, A. K., Gordon, M. J., and Leishman, D. J. (1993) Purification and partial characterisation of an alpha-tocopherol-binding protein from rabbit heart cytosol. Mol. Cell. Biochem. 123(1/2), 139.
13. Dutta-Roy, A. K., et al. (1993) Identification of a low molecular mass (14.2 kDa) alpha-tocopherol-binding protein in the cytosol of rat liver and heart. Biochem. Biophys. Res. Commun. 196(3), 1108-1112.
14. Gordon, M., et al. (1995) Characterization of a novel alpha-tocopherol-binding protein from bovine heart cytosol. Arch. Biochem. Biophys. 318(1), 140-146.
15. Hosomi, A., et al. (1997) Affinity for alpha tocopherol transfer protein as a determinant of the biological activities of vitamin E analogs. FEBS Lett. 409(1), 105-108.
16. Leonard, D. A., et al. (1998) Biochemical studies of the mechanism of action of the Cdc42-GTPase-activating protein. J. Biol. Chem. 273(26), 16210-16215.
17. Dutta-Roy, A. K. (1997) α-Tocopherol-binding proteins: Purification and characterization. Methods Enzymol. 282, 278-297.
18. Timmers, A. M. M., et al. (1986) A rapid versatile microassay for cellular retinol-binding protein using Lipidex-1000 microcolumns. J. Lipid Res. 27, 979-987.
19. Kramer, G., et al. (1999) N-terminal and C-terminal modifications affect folding, release from the ribosomes and stability of in vitro synthesized proteins. Int. J. Biochem. Cell. Biol. 31(1), 231-241.
20. Lin, L.-L., and Chu, W.-S. (1995) Expression of Pseudomonas amyloderamosa isoamylase gene in Escherichia coli using inducible T7 phage expression system. Proc. Biochem. 30(6), 547-552.
21. Appel, R. D., Bairoch, A., and Hochstrasser, D. F. (1994) A new generation of information retrieval tools for biologists: The example of the ExPASy WWW server. Trends Biochem. Sci. 19, 258-260.
22. Kyte, J., and Doolittle, R. F. (1982) A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157(1), 105-32.
23. Ikai, A. (1980) Thermostability and aliphatic index of globular proteins. J. Biochem. 88(6), 1895-1898.
24. Rost, B., and Sander, C. Prediction of protein secondary structure at better than 70% accuracy. J. Mol. Biol. 232(2), 584-599.
25. Chen, G. Q., and Gouaux, E. (1997) Overexpression of a glutamate receptor (GluR2) ligand binding domain in Escherichia coli: Application of a novel protein folding screen. Proc. Natl. Acad. Sci. USA 94(25), 13431-13436.
26. Baskakov, I., and Bolen, D. W. (1998) Forcing thermodynamically unfolded proteins to fold. J. Biol. Chem. 273(9), 4831-4834.
27. Daugherty, D. L., et al. (1998) Artificial chaperone-assisted refolding of citrate synthase. J. Biol. Chem. 273(51), 33961-33971.
28. Rozema, D., and Gellman, S. H. (1996) Artificial chaperone-assisted refolding of carbonic anhydrase B. J. Biol. Chem. 271(7), 3478-3487.
29. Nagata, Y., et al. (1999) Oxidation of d-alpha-tocopherol in aqueous solution. Formation of colored products. Chem. Pharm. Bull. 47 (7), 923-927.
30. Gordon, M. J., et al. (1995) Characterization of a novel alpha-tocopherol-binding protein from bovine heart cytosol. Arch. Biochem. Biophys. 318(1), 140-146.
31. Glatz, J. F. C., and Veerkamp, J. H. (1983) A radiochemical procedure for the assay of fatty acid binding by proteins. Anal. Biochem. 132, 89-95.
32. Ellis, R. J., and Hartl, F. U. (1999) Principles of protein folding in the cellular environment. Curr. Opin. Struct. Biol. 9(1), 102-110.
33. Qu, Y., Bolen, C. L., and Bolen, D. W. (1998) Osmolyte-driven contraction of a random coil protein. Proc. Natl. Acad. Sci. USA 95 (16), 9268-9273.
34. Baskakov, I., Wang, A., and Bolen, D. W. (1998) Trimethylamine-N-oxide counteracts urea effects on rabbit muscle lactate dehydrogenase function: A test of the counteraction hypothesis. Biophys. J. 74(5), 2666-2673.
35. Pitha, J. (1981) Enhanced water solubility of vitamins A, D, E, and K by substituted cycloamyloses. Life Sci. 29(3), 307-311.