cDNA cloning, bacterial expression, in vitro renaturation and affinity purification of the zinc endopeptidase astacin

Biochemical Journal

Volumn 344, Issue 3, 1999, Pages 851-857

  Reyda, Sabine ^a,c   Jacob, Elard ^b   Zwilling, Robert ^a   Stöcker, Walter ^a,d  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b BASF SE   (Germany)

^c PHILIPPS UNIVERSITY MARBURG   (Germany)

^d UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Astacins; Metalloproteases; Metzincins; Protein folding

Indexed keywords

ASTACIN; COMPLEMENTARY DNA; EDETIC ACID; ZINC ION;

ARTICLE; CELL INCLUSION; ENZYME PURIFICATION; ESCHERICHIA COLI; FLUORESCENCE ANALYSIS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN FOLDING; RENATURATION; REVERSE TRANSCRIPTION;

AMINO ACID SEQUENCE; ANIMALS; ASTACOIDEA; BASE SEQUENCE; BINDING SITES; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ESCHERICHIA COLI; KINETICS; METALLOENDOPEPTIDASES; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; PROTEIN FOLDING; RECOMBINANT PROTEINS; RNA, MESSENGER; ZINC;

EID: 0033572635     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3440851     Document Type: Article

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