A non-dechlorinating strain of Dehalospirillum multivorans: Evidence for a key role of the corrinoid cofactor in the synthesis of an active tetrachloroethene dehalogenase

Archives of Microbiology

Volumn 178, Issue 6, 2002, Pages 443-449

  Siebert, Anke ^a   Neumann, Anke ^a   Schubert, Torsten ^a   Diekert, Gabriele ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

Author keywords

Corrinoid; Dehalorespiration; Dehalospirillum multivorans; PCE; pceA; pceB; Strain K; Strain N; Tetrachloroethene; Tetrachloroethene reductive dehalogenase

Indexed keywords

APOPROTEIN; CELL EXTRACT; CORRINOID; ENZYME; TETRACHLOROETHANE; TETRACHLOROETHANE DEHALOGENASE; UNCLASSIFIED DRUG; OXIDOREDUCTASE; PORPHYRIN; TETRACHLOROETHENE DEHALOGENASE;

ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM; BACTERIUM ISOLATION; DECHLORINATION; DEHALOSPIRILLUM MULTIVORANS; DNA DETERMINATION; DNA SEQUENCE; ENZYME SYNTHESIS; GENE EXPRESSION; GENETIC TRANSCRIPTION; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING; ENZYMOLOGY; EPSILONPROTEOBACTERIA; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM; MOLECULAR GENETICS; PHYSIOLOGY;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; SULFUROSPIRILLUM; SULFUROSPIRILLUM MULTIVORANS;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CORRINOIDS; EPSILONPROTEOBACTERIA; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES; PORPHYRINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 0036428776     PISSN: 03028933     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00203-002-0473-8     Document Type: Article

References (21)

1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidmann JG, Smith JA, Struhl K (1999) Short Protocols in Molecular Biology. Wiley, New York
2. Birnboim HC, Doly J (1979) A rapid alkaline procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513-1523
3. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
4. Fennell ED, Carroll AB, Gossett JM, Zinder SH (2001) Assessment of indigenous reductive dechlorinating potential at a TCE contaminated site using microcosms, polymerase chain reaction analysis and site data. Environ Sci Technol 35:1830-1839
5. 12 und verwandte Corrinoide. In: Ammon R, Dirscherl W (eds) Fermente-Hormone-Vitamine und die Beziehungen dieser Wirkstoffe zueinander, band III/2. Thieme, Stuttgart, pp 10-48
6. Holliger C, Wohlfarth G, Diekert D. (1999) Reductive dechlorination in the energy metabolism of anaerobic bacteria. FEMS Microbiol Rev 22:383-398
7. Johnson JR, O'Bryan TT (2000) Improved repetitive-element PCR fingerprinting for resolving pathogenic and nonpathogenic phylogenetic groups within Escherichia coli. Clin Diagn Lab Immunol 7(2):265-273
8. Löffler FE, Sun Q, Li J, Tiedje JM (2000) 16S rRNA gene-based detection of tetrachloroethene dechlorinating Desulfuromonas and Dehalococcoides species. Appl Environ Microbiol 66:1369-1374
9. Neumann A, Scholz-Muramatsu H, Diekert G (1994) Tetrachloroethene metabolism of Dehalospirillum multivorans. Arch Microbiol 162:295-301
10. Neumann A, Wohlfarth G, Diekert G (1995) Properties of tetrachloroethene and trichloroethene dehalogenase of Dehalospirillum multivorans. Arch Microbiol 163:276-281
11. Neumann A, Wohlfarth G, Diekert G (1996) Purification and characterization of tetrachloroethene reductive dehalogenase from Dehalospirillum multivorans. J Biol Chem 271:16515-16519
12. Neumann A, Wohlfarth G, Diekert G (1998) Tetrachloroethene de-halogenase from Dehalospirillum multivorans: Cloning, sequencing of the encoding genes, and expression of the pceA gene in E. coli. J Bacteriol 180:4140-4145
13. Neumann A, Siebert A, Trescher T, Reinhard S, Wohlfarth G, Diekert G (2002) Tetrachloroethene reductive dehalogenase of Dehalospirillum multivorans: Substrate specificity of the native enzyme and the corrinoid cofactor. Arch Microbiol 177:420-426
14. Pas BA van de, Harmsen HJM, Raangs GC, Vos WM de, Schraa G, Stams AJM (2001) A Desulfitobacterium strain isolated from human feces that does not dechlorinate chloroethenes or chlorophenols Arch Microbiol 175:389-394
15. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd Edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
16. Scholz-Muramatsu H, Neumann A, Meßmer M, Moore E, Diekert G (1995) Isolation and characterization of Dehalospirillum multivorans gen. nov., sp. nov., a tetrachloroethene-utilizing, strictly anaerobic bacterium. Arch Microbiol 163:48-56
17. Smidt H, Akkermans AD, Oost J van der, Vos WM de (2000) Halorespiring bacteria-molecular characterization and detection. Enzyme Microb Technol 27:812-820
18. Stupperich E, Steiner I, Rühlemann M (1986) Isolation and analysis of bacterial cobamides by high-performance liquid chromatography. Anal Biochem 155:365-370
19. Stupperich E, Eisinger HJ, Kräutler B (1988) Diversity of corrinoids in acetogenic bacteria. Eur J Biochem 172:459-464
20. Versalovic J, Koeuth T, Lupski JR (1991) Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19:6823-6831
21. 12. Wiley, New York, pp 871-893