Cryo-negative staining reduces electron-beam sensitivity of vitrified biological particles

Journal of Structural Biology

Volumn 138, Issue 3, 2002, Pages 216-226

  De Carlo, S ^a   El Bez, C ^b   Alvarez Rua C ^c   Borge, J ^c   Dubochet, J ^b  

^a Centre de Microscopie Electronique   (Switzerland)

^b UNIVERSITY OF LAUSANNE   (Switzerland)

^c UNIVERSITY OF OVIEDO   (Spain)

Author keywords

3D reconstruction; Beam damage; Cryo negative staining; Cryoelectron microscopy; Fitting; GroEL; Specimen preparation; Vitrification

Indexed keywords

CHAPERONIN; PROTEIN SUBUNIT;

ARTICLE; CRYOELECTRON MICROSCOPY; ESCHERICHIA COLI; MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STRUCTURE; STAINING; THREE DIMENSIONAL IMAGING; X RAY CRYSTALLOGRAPHY;

CRYOELECTRON MICROSCOPY; CRYSTALLOGRAPHY, X-RAY; ELECTRONS; ESCHERICHIA COLI; GROEL PROTEIN; IMAGE PROCESSING, COMPUTER-ASSISTED; MODELS, MOLECULAR; MODELS, STATISTICAL; SPECIMEN HANDLING;

ESCHERICHIA COLI;

EID: 0036427905     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1047-8477(02)00035-7     Document Type: Article

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