메뉴 건너뛰기




Volumn 25, Issue 2, 2002, Pages 219-228

Expression and purification of catalytically active, non-toxic endopeptidase derivatives of clostridium botulinum toxin type A

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALS; BIOLOGICAL TRANSPORT; BLOTTING, WESTERN; BOTULINUM TOXIN TYPE A; CATALYSIS; CLOSTRIDIUM BOTULINUM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; MICE; NEUROMUSCULAR AGENTS; NEUROTRANSMITTER AGENTS; RABBITS; RECOMBINANT PROTEINS;

EID: 0036426323     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00002-5     Document Type: Article
Times cited : (62)

References (30)
  • 3
    • 0022416338 scopus 로고
    • Inactivation of Clostridium botulinum type A neurotoxin by trypsin and purification of two tryptic fragments. Proteolytic action near the COOH- terminus of the heavy subunit destroys toxin-binding activity
    • C.C. Shone, P. Hambleton, J. Melling, Inactivation of Clostridium botulinum type A neurotoxin by trypsin and purification of two tryptic fragments. Proteolytic action near the COOH- terminus of the heavy subunit destroys toxin-binding activity, Eur. J. Biochem. 151 (1985) 75-82
    • (1985) Eur. J. Biochem , vol.151 , pp. 75-82
    • Shone, C.C.1    Hambleton, P.2    Melling, J.3
  • 4
    • 0019619833 scopus 로고
    • The origin, structure, pharmacological activity of botulinum toxin
    • L.L. Simpson, The origin, structure, pharmacological activity of botulinum toxin, Pharmacol. Rev. 33 (1981) 155-188
    • (1981) Pharmacol. Rev , vol.33 , pp. 155-188
    • Simpson, L.L.1
  • 5
    • 0028341442 scopus 로고
    • Identification of protein receptor for Clostridium botulinum type B neurotoxin in rat brain synaptosomes
    • T. Nishiki, Y. Kamata, Y. Nemoto, A. Omori, T. Ito, M. Takahashi, S. Kozaki, Identification of protein receptor for Clostridium botulinum type B neurotoxin in rat brain synaptosomes, J. Biol. Chem. 269 (1994) 10498-10503
    • (1994) J. Biol. Chem , vol.269 , pp. 10498-10503
    • Nishiki, T.1    Kamata, Y.2    Nemoto, Y.3    Omori, A.4    Ito, T.5    Takahashi, M.6    Kozaki, S.7
  • 6
    • 0031663156 scopus 로고    scopus 로고
    • Ganglioside GT1b as a complementary receptor component for Clostridium botulinum neurotoxins
    • S. Kozaki, Y. Kamata, S. Watarai, T. Nishiki, S. Mochida, Ganglioside GT1b as a complementary receptor component for Clostridium botulinum neurotoxins, Microb. Pathog. 25 (1998) 91-99
    • (1998) Microb. Pathog , vol.25 , pp. 91-99
    • Kozaki, S.1    Kamata, Y.2    Watarai, S.3    Nishiki, T.4    Mochida, S.5
  • 7
    • 0029115961 scopus 로고
    • Formation of ion channels in lipid bilayers by a peptide with the predicted transmembrane sequence of botulinum neurotoxin A
    • M. Oblatt-Montal, M. Yamazaki, R. Nelson, M. Montal, Formation of ion channels in lipid bilayers by a peptide with the predicted transmembrane sequence of botulinum neurotoxin A, Protein Sci. 4 (1995) 1490-1497
    • (1995) Protein Sci , vol.4 , pp. 1490-1497
    • Oblatt-Montal, M.1    Yamazaki, M.2    Nelson, R.3    Montal, M.4
  • 8
    • 0026502052 scopus 로고
    • Clostridial neurotoxins proposal of a common nomenclature
    • J. Aguilera, Clostridial neurotoxins proposal of a common nomenclature, FEMS Lett. 90 (1992) 99-100
    • (1992) FEMS Lett , vol.90 , pp. 99-100
    • Aguilera, J.1
  • 9
    • 0023658427 scopus 로고
    • 2-terminus of the heavy subunit of Clostridium botulinum type A neurotoxin forms channels in lipid vesicles
    • 2-terminus of the heavy subunit of Clostridium botulinum type A neurotoxin forms channels in lipid vesicles, Eur. J. Biochem. 167 (1987) 175-180
    • (1987) Eur. J. Biochem , vol.167 , pp. 175-180
    • Shone, C.C.1    Hambleton, P.2    Melling, J.3
  • 10
    • 0028810969 scopus 로고
    • Expression and purification of the light chain of botulinum neurotoxin A: A single mutation abolishes its cleavage of SNAP-25 and neurotoxicity after reconstitution with the heavy chain
    • L. Zhou, A. de Paiva, D. Liu, R. Aoki, J.O. Dolly, Expression and purification of the light chain of botulinum neurotoxin A: A single mutation abolishes its cleavage of SNAP-25 and neurotoxicity after reconstitution with the heavy chain, Biochemistry 34 (1995) 15175-15181
    • (1995) Biochemistry , vol.34 , pp. 15175-15181
    • Zhou, L.1    De Paiva, A.2    Liu, D.3    Aoki, R.4    Dolly, J.O.5
  • 11
    • 0032953048 scopus 로고    scopus 로고
    • Proteolysis of SNAP-25 isoforms by botulinum neurotoxin types A, C, E: Domains and amino acid residues controlling the formation of enzyme-substrate complexes and cleavage
    • V.V. Vaidyanathan, K-I. Yoshino, M. Jahnz, C. Dorries, S. Bade, S. Nauenburg, H. Niemann, T. Binz, Proteolysis of SNAP-25 isoforms by botulinum neurotoxin types A, C, E: Domains and amino acid residues controlling the formation of enzyme-substrate complexes and cleavage, J. Neurochem. 72 (1999) 327-337
    • (1999) J. Neurochem , vol.72 , pp. 327-337
    • Vaidyanathan, V.V.1    Yoshino, K.-I.2    Jahnz, M.3    Dorries, C.4    Bade, S.5    Nauenburg, S.6    Niemann, H.7    Binz, T.8
  • 12
    • 0031282024 scopus 로고    scopus 로고
    • Recombinant expression and purification of the botulinum neurotoxin type A translocation domain
    • D.B. Lacy, R.C. Stevens, Recombinant expression and purification of the botulinum neurotoxin type A translocation domain, Protein Expr. Purif. 11 (1997) 195-200
    • (1997) Protein Expr. Purif , vol.11 , pp. 195-200
    • Lacy, D.B.1    Stevens, R.C.2
  • 13
    • 0029039466 scopus 로고
    • Protective vaccination with a recombinant fragment of Clostridium botulinum neurotoxin serotype A expressed from a synthetic gene in Escherichia coli
    • M.A. Clayton, J.M. Clayton, D.R. Brown, J.L. Middlebrook, Protective vaccination with a recombinant fragment of Clostridium botulinum neurotoxin serotype A expressed from a synthetic gene in Escherichia coli, Infect. Immun. 63 (1995) 2738-2742
    • (1995) Infect. Immun , vol.63 , pp. 2738-2742
    • Clayton, M.A.1    Clayton, J.M.2    Brown, D.R.3    Middlebrook, J.L.4
  • 14
    • 0029161741 scopus 로고
    • Growth of clostridia and preparation of their neurotoxins
    • C.C. Shone, H.S. Tranter, Growth of clostridia and preparation of their neurotoxins, Curr. Top. Microbiol. Immunol. 195 (1995) 143-160
    • (1995) Curr. Top. Microbiol. Immunol , vol.195 , pp. 143-160
    • Shone, C.C.1    Tranter, H.S.2
  • 15
    • 0000756769 scopus 로고
    • Characterisation of monoclonal antibodies to Botulinum type A neurotoxin
    • B.R. DasGupta (Ed.), Plenum Press, New York
    • B. Hallis, S. Fooks, C. Shone, P. Hambleton, Characterisation of monoclonal antibodies to Botulinum type A neurotoxin, in: B.R. DasGupta (Ed.), Botulinum and Tetanus Neurotoxins, Plenum Press, New York, 1993, pp. 433-436
    • (1993) Botulinum and Tetanus Neurotoxins , pp. 433-436
    • Hallis, B.1    Fooks, S.2    Shone, C.3    Hambleton, P.4
  • 17
    • 0020046078 scopus 로고
    • Preparation and characterisation of homogeneous neurotoxin type A from Clostridium botulinum. Its inhibitory action on neuronal release of acetylcholine in the absence and presence of beta-bungarotoxin
    • C.K. Tse, J.O. Dolly, P. Hambleton, D. Wray, J. Melling, Preparation and characterisation of homogeneous neurotoxin type A from Clostridium botulinum. Its inhibitory action on neuronal release of acetylcholine in the absence and presence of beta-bungarotoxin, Eur. J. Biochem. 122 (1982) 493-500
    • (1982) Eur. J. Biochem , vol.122 , pp. 493-500
    • Tse, C.K.1    Dolly, J.O.2    Hambleton, P.3    Wray, D.4    Melling, J.5
  • 18
    • 0017669602 scopus 로고
    • Mouse spinal cord in cell culture. I. Morphology and intrinsic neuronal electrophysiologic properties
    • B.R. Ransom, E. Neale, M. Henkart, P.N. Bullock, P.G. Nelson, Mouse spinal cord in cell culture. I. Morphology and intrinsic neuronal electrophysiologic properties, J. Neurophysiol. 40 (1977) 1132-1150
    • (1977) J. Neurophysiol , vol.40 , pp. 1132-1150
    • Ransom, B.R.1    Neale, E.2    Henkart, M.3    Bullock, P.N.4    Nelson, P.G.5
  • 20
    • 0029980484 scopus 로고    scopus 로고
    • Clostridial neurotoxins and substrate proteolysis in intact neurons: Botulinum neurotoxin C acts on synaptosomal-associated protein of 25 kDa
    • L.C. Williamson, J.L. Halpern, C. Montecucco, J.E. Brown, E.A. Neale, Clostridial neurotoxins and substrate proteolysis in intact neurons: Botulinum neurotoxin C acts on synaptosomal-associated protein of 25 kDa, J. Biol. Chem. 271 (1996) 7694-7699
    • (1996) J. Biol. Chem , vol.271 , pp. 7694-7699
    • Williamson, L.C.1    Halpern, J.L.2    Montecucco, C.3    Brown, J.E.4    Neale, E.A.5
  • 21
    • 0029893774 scopus 로고    scopus 로고
    • Development of novel assays for Botulinum type A and B neurotoxins based on their endopeptidase activities
    • B. Hallis, B.A.F. James, C.C. Shone, Development of novel assays for Botulinum type A and B neurotoxins based on their endopeptidase activities, J. Clin. Microbiol. 34 (1996) 1934-1938
    • (1996) J. Clin. Microbiol , vol.34 , pp. 1934-1938
    • Hallis, B.1    James, B.A.F.2    Shone, C.C.3
  • 22
    • 0002923670 scopus 로고
    • Assays for botulinum and tetanus toxins
    • L.L. Simpson (Ed.), Academic Press, New York
    • S. Notermans, J. Nagel, Assays for botulinum and tetanus toxins, in: L.L. Simpson (Ed.), Botulinum Neurotoxin and Tetanus Toxin, Academic Press, New York, 1989, pp. 319-331
    • (1989) Botulinum Neurotoxin and Tetanus Toxin , pp. 319-331
    • Notermans, S.1    Nagel, J.2
  • 24
    • 0025676110 scopus 로고
    • Light chain of botulinum neurotoxin is active in mammalian motor nerve terminals when delivered via liposomes
    • A. de Pavia, J.O. Dolly, Light chain of botulinum neurotoxin is active in mammalian motor nerve terminals when delivered via liposomes, FEBS Lett. 277 (1990) 171-174
    • (1990) FEBS Lett , vol.277 , pp. 171-174
    • De Pavia, A.1    Dolly, J.O.2
  • 25
    • 0034080288 scopus 로고    scopus 로고
    • Cloning, expression, one-step purification of the minimal essential domain of the light chain of botulinum neurotoxin type A
    • S. Kadhodayan, M.S. Knapp, J.J. Schmidt, S.E. Fabes, B. Rupp, R. Balhorn, Cloning, expression, one-step purification of the minimal essential domain of the light chain of botulinum neurotoxin type A, Protein Expr. Purif. 19 (2000) 125-130
    • (2000) Protein Expr. Purif , vol.19 , pp. 125-130
    • Kadhodayan, S.1    Knapp, M.S.2    Schmidt, J.J.3    Fabes, S.E.4    Rupp, B.5    Balhorn, R.6
  • 26
    • 0031712779 scopus 로고    scopus 로고
    • Crystal structure of botulinum neurotoxin type A and implications for toxicity
    • D.B. Lacy, W. Tepp, A.C. Cohen, B.R. DasGupta, R.C. Stevens, Crystal structure of botulinum neurotoxin type A and implications for toxicity, Nat. Struct. Biol. 5 (1998) 898-902
    • (1998) Nat. Struct. Biol , vol.5 , pp. 898-902
    • Lacy, D.B.1    Tepp, W.2    Cohen, A.C.3    DasGupta, B.R.4    Stevens, R.C.5
  • 27
    • 0033520494 scopus 로고    scopus 로고
    • Sequence homology and structural analysis of the clostridial neurotoxins
    • D.B. Lacy, R.C. Stevens, Sequence homology and structural analysis of the clostridial neurotoxins, J. Mol. Biol. 291 (1999) 1091-1104
    • (1999) J. Mol. Biol , vol.291 , pp. 1091-1104
    • Lacy, D.B.1    Stevens, R.C.2
  • 28
    • 0033748727 scopus 로고    scopus 로고
    • Development of vaccines for prevention of botulism
    • M.P. Byrne, L.A. Smith, Development of vaccines for prevention of botulism, Biochimie 82 (2000) 955-966
    • (2000) Biochimie , vol.82 , pp. 955-966
    • Byrne, M.P.1    Smith, L.A.2
  • 29
    • 0034114106 scopus 로고    scopus 로고
    • Inhibition of vesicular secretion in both neuronal and nonneuronal cells by a retargeted endopeptidase derivative of Clostridium botulinum neurotoxin type A
    • J.A. Chaddock, J.R. Purkiss, L.M. Friis, J.D. Broadbridge, M.J. Duggan, S.J. Fooks, C.C. Shone, C.P. Quinn, K.A. Foster, Inhibition of vesicular secretion in both neuronal and nonneuronal cells by a retargeted endopeptidase derivative of Clostridium botulinum neurotoxin type A, Infect. Immun. 68 (2000) 2587-2593
    • (2000) Infect. Immun , vol.68 , pp. 2587-2593
    • Chaddock, J.A.1    Purkiss, J.R.2    Friis, L.M.3    Broadbridge, J.D.4    Duggan, M.J.5    Fooks, S.J.6    Shone, C.C.7    Quinn, C.P.8    Foster, K.A.9
  • 30
    • 0033800350 scopus 로고    scopus 로고
    • A conjugate composed of nerve growth factor coupled to a non-toxic derivative of Clostridium botulinum neurotoxin type A can inhibit neurotransmitter release in vitro
    • J.A. Chaddock, J.R. Purkiss, M.J. Duggan, C.P. Quinn, C.C. Shone, K.A. Foster, A conjugate composed of nerve growth factor coupled to a non-toxic derivative of Clostridium botulinum neurotoxin type A can inhibit neurotransmitter release in vitro, Growth Factors 18 (2000) 147-155
    • (2000) Growth Factors , vol.18 , pp. 147-155
    • Chaddock, J.A.1    Purkiss, J.R.2    Duggan, M.J.3    Quinn, C.P.4    Shone, C.C.5    Foster, K.A.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.