Immunolocalization of vestitone reductase and isoflavone reductase, two enzymes involved in the biosynthesis of the phytoalexin medicarpin

Physiological and Molecular Plant Pathology

Volumn 61, Issue 1, 2002, Pages 15-30

  López Meyer, Melina ^a   Paiva, Nancy L ^a  

^a PLANT BIOLOGY DIVISION   (United States)

Author keywords

Alfalfa; Immunolocalization; Isoflavonoid biosynthesis; Medicago sativa; Medicarpin; Phytoalexin

Indexed keywords

MEDICAGO; MEDICAGO SATIVA; NICOTIANA TABACUM;

EID: 0036424956     PISSN: 08855765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0885-5765(02)90413-3     Document Type: Article

References (53)

1. Baker CJ, O'Neill NR, Tomerlin JR. 1989. Accumulation of phenolic compounds in incompatible clone/race interactions of Medicago sativa and Colletotrichum trifolii. Physiological and Molecular Plant Pathology 35: 231-241.
2. Barz W, Mackenbrock U. 1994. Constitutive and elicitation induced metabolism of isoflavones and pterocarpans in chickpea (Cicer arietinum) cell suspension cultures. Plant Cell Tissue and Organ Culture 38: 199-211.
3. Barz W, Welle R. 1992. Biosynthesis and metabolism of isoflavones and pterocarpan phytoalexins in chickpea, soybean and phytopathogenic fungi. In: Stafford HA, Ibrahim RK, eds. Phenolic Metabolism in Plants, Vol 26. New York, NY, U.S.A.: Plenum Press, 139-164.
4. Beerhues L, Robenek H, Wiermann R. 1988. Chalcone synthase from spinach (Spinacia oleracea L.) II. Immunofluorescence and immunogold localization. Planta 173: 544-553.
5. Bless W, Barz W. 1988. Isolation of pterocarpan synthase, the terminal enzyme of pterocarpan phytoalexin biosynthesis in cell suspension cultures of Cicer arietinum. FEBS Letters 235: 47-50.
6. Blount JW, Dixon RA, Paiva NL. 1992. Stress responses in alfalfa (Medicago sativa L.) XVI. Antifungal activity of medicarpin and its biosynthetic precursors; implications for the genetic manipulation of stress metabolites. Physiological and Molecular Plant Pathology 41: 333-349.
7. Chrispeels MJ, Raikhel NV. 1992. Short peptide domains target proteins to plant vacuoles. Cell 68: 613-616.
8. Dittrich H, Kutchan TM. 1991. Molecular cloning, expression, and induction of berberine bridge enzyme, an enzyme essential to the formation of benzophenanthridine alkaloids in the response of plants to pathogenic attack. Proceedings of the National Academy of Sciences of the U.S.A. 88: 9969-9973.
9. Dixon RA, Paiva NL. 1995. Stress-induced phenylpropanoid metabolism. The Plant Cell 7: 1085-1097.
10. Dixon RA, Paiva NL, Bhattacharyya MK. 1995. Engineering disease resistance in plants: an overview. In: Singh RP, Singh US, eds. Molecular Methods in Plant Pathology. Boca Raton, FL, U.S.A.: CRC Press, 249-270.
11. Dixon RA, Ghoundhary AD, Dalkin K, Edwards R, Fahrendorf T, Gowni G, Harrison MJ, Lamb CJ, Loake GJ, Maxwell CA, Orr J, Paiva NL. 1992. Molecular biology of stress-induced phenylpropanoid and isoflavonoid biosynthesis in alfalfa. In: Stafford HA, Ibrahim RK, eds. Phenolic Metabolism in Plants, Vol 26. New York, NY, U.S.A.: Plenum Press, 91-138.
12. Dewick PM. 1994. Isoflavonoids. In: HarborneJB, ed. The Flavonoids. London, U.K.: Chapman & Hall, 117-232.
13. Drews GN, Beals TP, Bui AQ, Goldberg RB. 1992. Regional and cell-specific gene expression patterns during plant development. The Plant Cell 4:1383-1404.
14. Fischer D, Ebenau-Jehle C, Grisebach H. 1990. Purification and characterization of pterocarpan synthase from elicitor-challenged soybean cell cultures. Phytochemistry 29: 2879-2882.
15. Guo L, Paiva NL. 1995. Molecular cloning and expression of alfalfa (Medicago sativa L.) vestitone reductase, the penultimate enzyme in medicarpin biosynthesis. Archives of Biochemistry and Biophysics 320:353-360.
16. Guo L, Dixon RA, Paiva NL. 1994a. Conversion of vestitone to medicarpin in alfalfa (Medicago sativa L.) is catalyzed by two independent enzymes. Journal of Biological Chemistry 269:22372-22378.
17. Guo L, Dixon RA, Paiva NL. 1994b. The "pterocarpan synthase" of alfalfa: Association and co-induction of vestitone reductase and 7,2′-dihydroxy-4′-methoxy-isoflavanol (DMI) dehydratase, the two final enzymes in medicarpin biosynthesis. FEBS Letters 356:221-225.
18. Hakamatsuka T, Mori K, Ishida S, Ebizuka Y, Sankawa U. 1998. Purification of 2-hydroxyisoflavanone dehydratase from the cell cultures of Pueraria lobata. Phytochemistry 49:497-505.
19. Hibi N, Higashiguchi S, Hashimoto T, Yamada Y. 1994. Gene expression in tobacco low-nicotine mutants. The Plant Cell 6:723-735.
20. Higgins VJ. 1972. Role of the phytoalexin medicarpin in three leaf spot diseases of alfalfa. [Stemphylium botryosum, Phoma herbarum medicaginis, Leptosphaerulina briosiana]. Physiological Plant Pathology 2:289-300.
21. Hipskind JD, Paiva NL. 2000. Constitutive accumulation of a resveratrol glucoside in transgenic alfalfa increases resistance to Phoma medicaginis. Molecular-Plant Microbe Interactions 3:551-562.
22. Hrazdina G. 1992. Compartmentation in aromatic metabolism. In: Stafford HA, Ibrahim RK, eds. Phenolic Metabolism in Plants, Vol 26. New York, NY, U.S.A.: Plenum Press, 1-24.
23. Hrazdina G, Wagner GJ. 1985. Metabolic pathways as enzyme complexes: Evidence for the synthesis of phenylpropanoids and flavonoids on membrane associated complexes. Archives of Biochemistry and Biophysics 237: 88-100.
24. Hrazdina G, Marx GA, Hoch HG. 1982. Distribution of secondary plant metabolites and their biosynthetic enzymes in pea (Pisum sativum L.) leaves. Plant Physiology 70:745-748.
25. Hrazdina G, Zobel A, Hoch HG. 1987. Biochemical, immunological, and immunocytochemical evidence for the association of chalcone synthase with endoplasmic reticulum membranes. Proceedings of the National Academy of Sciences, U.S.A. 84:8966-8970.
26. Ibrahim RK. 1992. Immunolocalization of flavonoids conjugates and their enzymes. In: Stafford HA, Ibrahim RK, eds. Phenolic Metabolism in Plants, Vol. 26. New York, NY, U.S.A.: Plenum Press, pp. 25-61.
27. Jahnen W, Hahlbrock K. 1988. Differential regulation and tissue-specific distribution of enzymes of phenylpropanoid pathways in developing parsley seedlings. Planta 173: 453-458.
28. Kessmann H, Choudhary AD, Dixon RA. 1990a. Stress responses in alfalfa (Medicago sativa L.). III. Induction of medicarpin and cytochrome P450 enzyme activities in elicitor-treated cell suspension cultures and protoplasts. Plant Cell Reports 9:38-41.
29. Kessmann H, Edwards R, Geno R, Dixon RA. 1990b. Stress responses in alfalfa (Medicago sativa L.) V. Constitutive and elicitor-induced accumulation ofisoflavonoid conjugates in cell suspension cultures. Plant Physiology 94: 227-232.
30. Mackenbrock U, Vogelsang R, Barz W. 1992. Isoflavone and ptcrocarpan malonylglucosidcs and β-1,3-glucanand chitin-hydrolases are vacuolar constituents in chickpea (Cicer arietinum L.). Zeitschrift für Naturforschung 47: 815-822.
31. Nakai K, Horton P. 1999. PSORT: A program for detecting sorting signals in proteins and predicting their subcellular localization. Trends in Biochemical Science 24: 34-36.
32. Oommen A, Dixon RA, Paiva NL. 1994. The elicitor-inducible alfalfa isoflavone reductase promoter confers different patterns of developmental expression in homologous and heterologous transgenic plants. The Plant Cell 6: 1789-1803.
33. Paiva NL, Edwards R, Sun Y, Hrazdina G, Dixon RA. 1991. Stress responses in alfalfa (Medicago sativa L.) XI. Molecular cloning and expression of alfalfa isoflavone reductase, a key enzyme of isoflavonoid phytoalexin biosynthesis. Plant Molecular Biology 17:653-667.
34. Parry A, Edwards R. 1994. Characterization of O-glucosyltransferases with activities toward phenolic substrates in alfalfa. Phytochemistry 37:655-661.
35. Peterson GL. 1977. A simplification of protein assay method of Lowry et al which is more generally applicable. Analytical Biochemistry 83:346-356.
36. Rasmussen S, Dixon RA. 1999. Transgene-mediated and elicitor-induced perturbation of metabolic channeling at the entry point into the phenylpropanoid pathway. The Plant Cell 11:1537-1552.
37. Restrepo MA, Freed DD, Garrington JG. 1990. Nuclear transport of plant potyviral proteins. The Plant Cell 2: 987-998.
38. Roland JG, Vian B. 1991. General preparation and staining of thin sections. In: Hall JL, Hawes C, eds. Electron Microscopy of Plant Cells. London, U.K.: Academic Press, 1-33.
39. Schmelzer E, Jahnen W, Hahlbrock K. 1988. In situ localization of light-induced chalcone synthase mRNA, chalcone synthase, and flavonoid end products in epidermal cells of parsley leaves. Proceedings of the National Academy of Sciences, U.S.A. 85:2989-2993.
40. Schmelzer E, Kruger-Lebus S, Hahlbrock K. 1989. Temporal and spatial patterns of gene expression around sites of attempted fungal infection in parsley leaves. The Plant Cell 1: 993-1001.
41. Schopker H, Kneisel M, Beerhues L, Robenek H, Wiermann R. 1995. Phenylalanine ammonia-lyase and chalcone synthase in glands of Primula kewensis (W. Wats): Immunofluorescence and immunogold localization. Planta 196:712-719.
42. Shorrosh BS, Subramaniam J, Schubert KR, Dixon RA. 1993. Expression and localization of plant protein disulfide isomerase. Plant Physiology 103:719-726.
43. Smith CG, Rodgers MW, Zimmerlin A, Ferdinando D, Bolwell GP. 1994. Tissue and subcellular immunolocalization of enzymes of lignin synthesis in differentiating and wounded hypocotyl tissue of French bean (Phaseolus vulgaris L.). Planta 192:155-164.
44. Snyder BA, Nicholson RL. 1990. Synthesis of phytoalexins in sorghum as a site specific response to fungal ingress. Science 248:1637-1639.
45. Stafford HA. 1990. Pathway to proanthocyanidins (condensed tannins), flavan-3-ols, and unsubstituted flavans. Flavonoid Metabolism. Boca Raton, FL, U.S.A.: CRC Press, 63-99.
46. Stoessl A. 1982. Biosynthesis of phytoalexins. In: Bailey JA, Mansfield JW, eds. Phytoalexins. New York, NY, U.S.A.: John Wiley & Sons, 133-180.
47. Stuteville DL, Erwin DC. 1990. Compendium of Alfalfa Diseases, 2nd Edn. St. Paul, MN, U.S.A.: APS Press, 1-84.
48. Sumner LW, Paiva NL, Dixon RA, Geno PW. 1996. High-performance liquid chromatography/continuous-flow liquid secondary ion mass spectrometry of flavonoid glucosides in leguminous plant extracts. Journal of Mass Spectrometry 31:472-485.
49. Sun Y, Wu Q, Van Etten HD, Hrazdina G. 1991. Stereoisomerism in plant disease resistance: Induction and isolation of the 7,2′-dihydroxy-4′,5′-methylenedioxyisoflavone oxidoreductase, an enzyme introducing chirality during synthesis of isoflavonoid phytoalexin in pea (Pisum sativum L). Archives of Biochemistry and Biophysics 284: 167-173.
50. Tamaki H, Yamashina S. 1994. Improved method for post embedding cytochemistry using reduced osmium and LR white resin. Journal of Histochemistry and Cytochemistry 42: 1285-1293.
51. VandenBosch KA, Sherrier DJ, Dreyer DA. 1995. Light microscopic applications of immunocytochemistry. In: Gelvin SB, Schilperoort RA, eds. Plant Molecular Biology Manual, 2nd Edn. Dordrecht, Netherlands: Kluwer Academic, Vol. G3:1-18.
52. Wagner GJ, Hrazdina G. 1984. Endoplasmic reticulum as a site of phenylpropanoid and flavonoid metabolism in Hippeastrum. Plant Physiology 74:901-906.
53. Wiermann R. 1981. Secondary plant products and cell and tissue differentiation. In: Stumpf PK, Conn EE, eds. The Biochemistry of Plants. New York, NY, U.S.A.: Academic Press, 85-116.