Characterization of α (2→6)-sialyltransferase reaction intermediates: Use of alternative substrates to unmask kinetic isotope effects

Methods in Enzymology

Volumn 354, Issue , 2002, Pages 159-168

  Horenstein, Benjamin A ^a   Bruner, Michael ^a  

^a University of Florida ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOSYLTRANSFERASE; SIALYLTRANSFERASE;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME MECHANISM; ENZYME METABOLISM; ENZYME SPECIFICITY; ENZYME STRUCTURE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; PRIORITY JOURNAL; VISCOSITY;

EID: 0036408137     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(02)54012-X     Document Type: Chapter

References (25)

1. B. A. Horenstein and M. Bruner, J. Am. Chem. Soc. 118, 10371 (1996).
2. B. A. Horenstein, J. Am. Chem. Soc. 119, 1101 (1997).
3. B. A. Horenstein and M. Bruner, J. Am. Chem. Soc. 120, 1357 (1998).
4. B. A. Horenstein, in "Transition-State Modeling for Catalysis," ACS Symp. Ser. #721 (D. G. Truhlar and K. Morokuma, eds.). American Chemical Society, Washington, DC, 1999.
5. M. Bruner and B. A. Horenstein, Biochemistry 37, 289 (1998).
6. M. Bruner and B. A. Horenstein, Biochemistry 39, 2261 (2000).
7. P. Salomaa, L. L. Schaleger, and F. A. Long, J. Am. Chem. Soc. 86, 1 (1964).
8. F. W. Dahlquist, T. Rand-Meir, and M. A. Raftery, Biochemistry 8, 4214 (1969).
9. D. L, Parkin, in "Enzyme Mechanisms from Isotope Effects" (P. F. Cook, ed.), p. 269. CRC Press, Boca Raton, FL, 1991.
10. R. G. Dugglesby and D. B. Northrop, Bioorg. Chem. 17, 177 (1989).
11. J. Bigeleisen and M. Wolfsberg, Adv. Phys. Chem. 1, 15 (1958).
12. J. Weinstein, U. Souza-e-Silva, and J. C. Paulson, J. Biol. Chem. 257, 13845 (1982).
13. I. Kirshenbaum, "Physical Properties and Analysis of Heavy Water," p. 33. McGraw-Hill, New York, 1951.
14. C. S. Miner and N. N. Dalton, "Glycerol," ACS Monograph Series, p. 240. Reinhold Publishing Corp., New York, 1953.
15. H. H. Higa, J. C. Paulson, and J. Weinstein, J. Biol. Chem. 260, 8838 (1985).
16. H. J. Gross, U. Rose, J. M. Krause, J. C. Paulson, K. Schmid, R. E. Feeney, and R. Brossmer, Biochemistry 28, 7386 (1989).
17. R. G. Kleineidam, T. Schmelter, R. T. Schwartz, and R. Schauer, Glycoconjugate J. 14, 57 (1997).
18. H. Simon and D. Palm, Angew. Chem., Int. Ed. Engl. 5, 920 (1966).
19. D. B. Northrop, in "Isotope Effects on Enzyme Catalyzed Reactions" (W. W. Cleland, M. H. O'Leary, and D. B, Northrop, eds.). University Park Press, Baltimore, 1977.
20. V. L. Schramm, Methods Enzymol. 308, 301 (1999).
21. L. Melander and W. H. Saunders, "Reaction Rates of Isotopic Molecules," Chapter 6. Kreiger, Malabar, FL, 1980.
22. R. K. Goiten, D. Chelsky, and S. M. Parsons, J. Biol. Chem. 253, 2963 (1978).
23. A. J. Bennett and M. L. Sinnott, J. Am. Chem. Soc. 108, 7287 (1986).
24. L. Melander and W. H. Saunders, in "Reaction Rates of Isotopic Molecules," p. 242. Kreiger, Malabar, FL, 1980.
25. J. P. Richard, R. E. Huber, C. Heo, T. L. Amyes, and S. Lin, Biochemistry 35, 12387 (1996).