Purification and kinetic analysis of the two recombinant arogenate dehydrogenase isoforms of Arabidopsis thaliana

European Journal of Biochemistry

Volumn 269, Issue 19, 2002, Pages 4753-4761

  Rippert, Pascal ^a   Matringe, Michel ^a  

^a BAYER AG   (Germany)

Author keywords

Arabidopsis thaliana; Arogenate dehydrogenase; Enzymatic properties; Isoforms; Tyrosine biosynthesis

Indexed keywords

AROGENATE DEHYDROGENASE; AROGENIC ACID; ISOENZYME; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; OXIDOREDUCTASE; PREPHENATE DEHYDROGENASE; PROTEIN PRECURSOR; RECOMBINANT ENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TYROSINE; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

AMINO ACID SYNTHESIS; ARABIDOPSIS; ARTICLE; CATALYSIS; DECARBOXYLATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME MECHANISM; ENZYME PURIFICATION; ESCHERICHIA COLI; OXIDATION; PRIORITY JOURNAL; VELOCITY;

AMINO ACID SEQUENCE; AMINO ACIDS, DICARBOXYLIC; ARABIDOPSIS; BASE SEQUENCE; CYCLOHEXENES; DNA, PLANT; ENZYME INHIBITORS; ESCHERICHIA COLI; ISOENZYMES; KINETICS; MOLECULAR SEQUENCE DATA; NADP; PREPHENATE DEHYDROGENASE; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TYROSINE;

ARABIDOPSIS; ARABIDOPSIS THALIANA; ESCHERICHIA COLI;

EID: 0036399671     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.03172.x     Document Type: Article

References (38)

1. Steinrucken, H.C. & Amrhein, N. (1980) The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic acid-3-phosphate synthase. Biochem. Biophys. Res. Commu. 94, 1207-1212.
2. Lingens, F., Goebel, W. & Uesseler, H. (1967) Regulation der aromatischen aminosâuren in Saccharomyces cerevisiae. Eur. J. Biochem. 1, 363-374.
3. Sampathkumar, P. & Morrisson, J.F. (1982) Chorismate mutase-prephenate dehydrogenase from Escherichia coli. Purification and properties of the bifunctional enzyme. Biochim. Biophys. Acta 702, 204-211.
4. Byng, G., Whitaker, R., Flick, C. & Jensen, R.A. (1981) Enzymology of 1-tyrosine biosynthesis in corn (Zea Mays). Phytochemistry 6, 1289-1292.
5. Gaines, C.G., Gyng, G.S., Whitaker, R.J. & Jensen, R.A. (1982) 1-tyrosine regulation and biosynthesis via arogenate dehydrogenase in suspension-cultured cells of Nicotiana silvestris. Speg. et Comes. Planta 156, 233-240.
6. Connelly, J.A. & Conn, E.E. (1986) Tyrosine biosynthesis in Sorghum bicolor: isolation and regulatory properties of arogenate dehydrogenase. Z. Naturforsch 41c, 69-78.
7. Bonner, C.A., Fisher, R.S., Schmidt, R.R., Miller, P.W. & Jensen, R.A. (1995) Distinct enzymes of aromatic amino acid biosynthesis that are highly conserved in land plants are present in the chlorophyte alga Chlorella sorokiniana. Plant Cell Physiol. 36, 1013-1022.
8. Abou-Zeid, A., Euverink, G.J.W., Hessels, G.I., Jensen, R.A. & Dijkhuizen, L. (1995) Biosynthesis of 1-phenylalanine and 1-tyrosine in the actinomycete Amycolatopsis methanolica. Appl. Env. Microbiol. 41, 1298-1302.
9. Fazel, A.M. & Jensen, R.A. (1979) Obligatory biosynthesis of 1-tyrosine via the pretyrosine branchlet in coryneform bacteria. J. Bacteriol. 138, 805-815.
10. Hall, G.C., Flick, M.B., Gherna, R.L. & Jensen, R.A. (1982) Biochemical diversity for biosynthesis of aromatic aminoacids among cyanobacteria. J. Bacteriol. 149, 65-78.
11. Keller, B., Keller, E. & Lingens, F. (1985) Arogenate dehydrogenase from Streptomyces phaeochromogenes. Purification and properties. Biol. Chem. Hoppe Seyler 366 (11), 1063-1066.
12. Mayer, E., Waldner-Sander, S., Keller, B., Keller, E. & Lingens, F. (1985) Purification of arogenate dehydrogenase from Phenylobacterium immobile. FEBS Lett. 792, 208-212.
13. Patel, N., Pierson, D.L. & Jensen, R.A. (1977) Dual enzymatic routes to 1-tyrosine and 1-phenylalanine via pretyrosine in Pseudomonas aeruginosa. J. Biol. Chem. 252, 5839-5846.
14. Zhao, G., Xia, T., Ingram, L.O. & Jensen, R.A. (1993) An allosterically insensitive class of cyclohexadienyl dehydrogenase from Zymomonas mobilis. Eur. J. Biochem. 212, 157-165.
15. Hermann, K.M. & Weaver, L.M. (1999) The shikimate pathway. Annu. Rev. Plant Physiol. Plant Mol. Biol. 50, 473-503.
16. Schmid, J. & Amrhein, N. (1995) Molecular organization of the shikimate pathway in higher plants. Phytochemistry 39 (4), 737-749.
17. Schmid, J. & Amrhein, N. (1999) The shikimate pathway. In Plant Amino Acids (Singh, B., ed.), pp. 147-169. Marcel Dekker, New York.
18. Wink, M. (1999) Plant secondary metabolites from higher plants: biochemistry, function and biotechnology. In Biochemistry of Plant Secondary Metabolism, Anual Plant Reviews (Wink, M., ed.), pp. 1-16. Sheffield Academics, Sheffield.
19. Rippert, P. & Matringe, M. (2002) Molecular and biochemical characterisation of an A. thaliana arogenate dehydrogenase with two highly similar and active domains. Plant Mol. Biol. 48, 361-368.
20. Arabidopsis Genome Initiative (2000) Analyses of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408, 796-815.
21. Heyde, E. & Morrison, J.F. (1978) Kinetic studies on the reactions catalysed by chorismate mutase-prephenate dehydrogenase from Aerobacter aerogenes. Biochemistry 17, 1573-1580.
22. Turnbull, J., Cleland, W.W. & Morrison, J.F. (1990) Chorismate mutase-prephenate dehydrogenase from Escherichia coli. 1. Kinetic characterization of the dehydrogenase reaction by use of alternative substrates. Biochemistry 29, 10245-10254.
23. Taylor, A.L. & Trotter, C.D. (1967) Revised linkage map of Escherichia C-Oli. Bacteriol. Rev. 31, 332-353.
24. Dower, W.J., Miller, J.F. & Ragsdate, C.W. (1988) High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 16, 6127-6145.
25. Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
26. Scopes, R.K. (1974) Measurement of protein by spectrophotometry at 205 nm. Anal. Biochem. 59, 277-282.
27. Chua, N.H. (1980) Electrophoresis analysis of chloroplast proteins. Methods Enzymol. 69, 434-436.
28. Jensen, R.A., Zamir, L.S.T., Pierre, M., Patel, N. & Pierson, D.L. (1977) Isolation and preparation of pretyrosine, accumulated as a dead-end metabolite by Neurospora crassa. J. Of Bacteriol. 132, 896-903.
29. Bonner, C.A. & Jensen, R.A. (1987) Arogenate dehydrogenase. Meth Enzymol. 142, 488-494.
30. Emanuelsson, O., Nielsen, H. & Von Heijne, G. (1999) ChloroP, a neutral network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Sci. 8, 978-984.
31. Rubin, J.L. & Jensen, R.A. (1979) Enzymology of 1-tyrosine in Mung Bean (Vigna radiata [L.] Wilczek). Plant Physiol. 64, 727-734.
32. Segel, I.H. (1993) Enzyme Kinetics, Wiley & Sons, Inc., New York.
33. Higuchi, T. (1985) Biosynthesis and Biodegradation of Wood Components, Academic Press, Orlando.
34. Siehl, D.L. (1999) The biosynthesis of tryptophan, tyrosine, and phenylalanine from chorismate. In Plant Amino Acids (Singh, B., ed.), pp. 171-204. Marcel Dekker, New York.
35. Christopherson, R.I. & Morrison, J.F. (1985) Chorismate mutase-prephenate dehydrogenase from Escherichia coli: positive cooperativity with substrates and inhibitors. Biochemistry 24, 1116-1121.
36. Koch, G.L., Shaw, D.C. & Gibson, F. (1971) The purification and characterisation of chorismate mutase-prephenate dehydrogenase from Escherichia coli K12. Biochim. Biophys. Acta 229, 795-804.
37. Turnbull, J., Morrison, J.F. & Cleland, W.W., (1991) Kinetic studies on chorismate mutase-prephenate dehydrogenase from Escherichia coli: models for the feedback inhibition of prephenate dehydrogenase by L-tyrosine. Biochemistry 30, 7783-7788.
38. Hund, H.K., Bär, G. & Lingens, F. (1989) Purification and properties of arogenate dehydrogenase of Actinoplanes mis-souriensis. Z. Naturforsch. 44c, 797-801.