Transduction of immortalized human hepatocytes with p21 to enhance differentiated phenotypes

Cell Transplantation

Volumn 11, Issue 5, 2002, Pages 421-428

  Kunieda, T ^a   Kobayashi, N ^b   Sakaguchi, M ^b   Okitsu, T ^b   Totsugawa, T ^b   Watanabe, T ^b   Matsumura, T ^b   Maruyama, M ^b   Noguchi, H ^b   Takesue, M ^a   Shibata, N ^a   Ohmoto, K ^a   Fujiwara, T ^b   Yamamoto, S ^a   Tanaka, N ^b  

^a KAWASAKI MEDICAL SCHOOL   (Japan)

^b OKAYAMA UNIVERSITY   (Japan)

Author keywords

Immortalized hepatocytes; p21; Protein transduction

Indexed keywords

ACTIN; ADENOVIRUS VECTOR; ALBUMIN; COMPLEMENTARY DNA; CYTOCHROME P450 2C9; CYTOCHROME P450 3A4; HYBRID PROTEIN; PROTEIN P21; TRANSACTIVATOR PROTEIN; VIRUS T ANTIGEN;

ARTICLE; CELL CYCLE; CELL IMMORTALIZATION; CONTROLLED STUDY; CYTOMEGALOVIRUS; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS; LIVER CELL; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; SIMIAN VIRUS 40;

ADENOVIRIDAE; CUCUMBER MOSAIC VIRUS; CYTOMEGALOVIRUS; HUMAN IMMUNODEFICIENCY VIRUS; SIMIAE; SIMIAN VIRUS 40;

EID: 0036387078     PISSN: 09636897     EISSN: None     Source Type: Journal    
DOI: 10.3727/000000002783985666     Document Type: Article

References (17)

1. El-Deiry, W. S.; Tokino, T.; Velculescu, V. E.; Levy, D. B.; Parsons, R.; Trent, J. M.; Lin, D.; Mercer, W. E.; Kinzler, K. W.; Vogelstein, B. WAF1, a potential mediator of p53 tumor suppression. Cell 75:817-825; 1993.
2. Estham, J. A.; Hall, S. J.; Sehgal, I.; Wang, J.; Timme, T. L.; Yang, G.; Connell, C. L.; Elledge, S. J.; Zhang, W. W.; Harper, J. W.; Thompson, T. C. In vivo gene therapy with p53 or p21 adenovirus for prostate cancer. Cancer Res. 55:5151-5155; 1995.
3. Frankel, A. D.; Pabo, C. O. Cellular uptake of the Tat protein from human immunodeficiency virus. Cell 55:1189-1193; 1988.
4. Green, M.; Loewenstein, P. M. Autonomous functional domains of chemically synthesized human immunodeficiency virus Tat trans-activator protein. Cell 55:1179-1188; 1988.
5. Halevy, O.; Novitch, B. G.; Spicer, D. B.; Skapek, S. X.; Rhee, J.; Hannon, G. J.; Beach, D.; Lassar, A. B. Correlation of terminal cell cycle arrest of skeletal muscle with induction of p21 by MyoD. Science 267:1018-1021; 1995.
6. Harper, J. W.; Adami, G. R.; Wei, N.; Keyomarsi, K.; Elledge, S. J. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinase. Cell 75:805-816; 1993.
7. Jiang, H.; Lin, J.; Su, Z. Z.; Herlyn, M.; Kerbel, R. S.; Weissman, B. E.; Welch, D. R.; Fisher, P. B. The melanoma differentiation-associated gene mda-6, which encodes the cyclin-dependent kinase inhibitor p21, is differentially expressed during growth, differentiation and progression in human melanoma cells. Oncogene 10:1855-1864; 1995.
8. Kagawa, S.; Fujiwara, T.; Hizuta, A.; Yasuda, T.; Zhang, W. W.; Roth, J. A.; Tanaka, N. p53 expression overcome p21 WAF1/CIP1-mediated G1 arrest and induces apoptosis in human cancer cells. Oncogene 15:1903-1909; 1997.
9. Kobayashi, N.; Fujiwara, T.; Westerman, K. A.; Inoue, Y.; Sakaguchi, M.; Noguchi, H.; Miyazaki, M.; Cai, J.; Tanaka, N.; Fox, I. J.; Leboulch, P. Prevention of acute liver failure in rats with reversibly immortalized human hepatocytes. Science 287:1258-1262; 2000.
10. Kobayashi, N.; Sakaguchi, M.; Okitsu, T.; Totsugawa, T.; Maruyama, M.; Matsumura, T.; Watanabe, T.; Noguchi, H.; Kosaka, Y.: Fujiwara, T.; Tanaka, N. Improvement in differentiated hepatic phenotype of immortalized human hepatocytes by adenovirus-mediated p21 gene transfer. ASAIO J. (in press).
11. Liu, M.; Lee, M. H.; Cohen, M.; Bommakanti, M.; Freedman, L. P. Transcriptional activation of the Cdk inhibitor p21 by vitamin D3 leads to the induced differentiation of the myelomonocytic cell line U937. Genes Dev. 10:142-153; 1995.
12. Marchetti, A.; Doglioni, C.; Barbareschi, M.; Buttitta, F.; Pellegrini, S.; Bertacca, G.; Chella, A.; Merlo, G.; Angeletti, C. A.; Dalla, P. P.; Bevilacqua, G. p21 RNA, and protein expression in non-small cell lung carcinomas: Evidence of p53-independent expression and association with tumoral differentiation. Oncogene 12:1319-1324; 1996.
13. Nagahara, H.; Vocero-Akbani, A. M.; Snyder, E. L.; Ho, A.; Latham, D. G.; Lissy, N. A.; Becker-Hapak, M.; Ezhevsky, S. A.; Dowdy, S. F. Transduction of full-length TAT fusion proteins into mammalian cells: TAT-p27Kipl induced cell migration. Nat. Med. 4:1449-1452; 1998.
14. Noda, A.; Ning, Y.; Venable, S. F.; Pereira, S. D.; Smith, J. R. Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen. Exp. Cell Res. 211:90-98; 1994.
15. Sakaguchi, M.; Miyazaki, M.; Inoue, Y.; Tsuji, T.; Kouchi, H.; Tanaka, T.; Yamada, H.; Namba, M. Relationship between contact inhibition and intranuclear S100C of normal human fibroblasts. J. Cell Biol. 149:1193-1206; 2000.
16. Sakaguchi, M.; Yamada, H.; Tsuji, T.; Inoue, Y.; Miyazaki, M.; Tanaka, T.; Namba, M. Loss of nuclear localization of the S100C protein in immortalized human fibroblasts. Radiat. Res. 154; 2000.
17. Vives, E. A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus. J. Biol. Chem. 272:16010-16017; 1997.