Expression of P450 enzymes in rat whole skin and cultured epidermal keratinocytes

Biochemical and Biophysical Research Communications

Volumn 297, Issue 1, 2002, Pages 65-70

  Zhu, Zheying ^a   Hotchkiss, Sharon A ^a   Boobis, Alan R ^a   Edwards, Robert J ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Cell culture; CYP; Cytochrome P450; Keratinocytes; Rat; Skin

Indexed keywords

CYTOCHROME P450; CYTOCHROME P450 1A1; CYTOCHROME P450 1A2; CYTOCHROME P450 2B12; CYTOCHROME P450 2C12; CYTOCHROME P450 2C13; CYTOCHROME P450 2D4; CYTOCHROME P450 2E1; CYTOCHROME P450 3A1; CYTOCHROME P450 3A2; ENZYME ANTIBODY; UNCLASSIFIED DRUG; XENOBIOTIC AGENT;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL GROWTH; CELL ISOLATION; CONTROLLED STUDY; ENZYME ASSAY; ENZYME LOCALIZATION; ENZYME METABOLISM; EPIDERMIS CELL; IMMUNOREACTIVITY; KERATINOCYTE; LIVER; MALE; MICROSOME; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SKIN;

ANIMALS; ARYL HYDROCARBON HYDROXYLASES; CELLS, CULTURED; CYTOCHROME P-450 CYP1A1; CYTOCHROME P-450 CYP2E1; CYTOCHROME P-450 ENZYME SYSTEM; GENE EXPRESSION PROFILING; KERATINOCYTES; MIXED FUNCTION OXYGENASES; RATS; SKIN; STEROID HYDROXYLASES;

EID: 0036382840     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(02)02134-4     Document Type: Article

References (25)

1. D.R. Bickers, H. Mukhtar, Skin as a portal entry for systemic effect of xenobiotic metabolism, in: C.L. Galli, C.N. Hensby, M. Marinovich (Eds.), Skin Pharmacology and Toxicology, Recent Advances, Plenum Press, New York, 1990, pp. 85-97.
2. D.R. Nelson, L. Koymans, T. Kamataki, J.J. Stegeman, R. Feyereisen, D.J. Waxman, M.R. Waterman, O. Gotoh, M.J. Coon, R.W. Estabrook, I.C. Gunsalus, D.W. Nebert, P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature, Pharmacogenetics 6 (1996) 1-42.
3. P.E. Thomas, S. Bandiera, S.L. Maines, D.E. Ryan, W. Levin, Regulation of cytochrome P-450j, a high-affinity N-nitrosodi-methylamine demethylase, in rat hepatic microsomes, Biochemistry 26 (1987) 2280-2289.
4. D. Sesardic, K.J. Cole, R.J. Edwards, D.S. Davies, P.E. Thomas, W. Levin, A.R. Boobis, The inducibility and catalytic activity of cytochromes P450c (P450IA1) and P450d (P450IA2) in rat tissues, Biochem. Pharmacol. 39 (1990) 499-506.
5. K. Debri, A.R. Boobis, D.S. Davies, R.J. Edwards, Distribution and induction of CYP3A1 and CYP3A2 in rat liver and extrahepatic tissues, Biochem. Pharmacol. 50 (1995) 2047-2056.
6. M.A. Pham, J. Magdalou, M. Totis, S. Fournel-Gigleux, G. Siest, B.D. Hammock, Characterization of distinct forms of cytochromes-P450, epoxide metabolizing enzymes and UDP-glucuronyltransferases in rat skin, Biochem. Pharmacol. 38 (1989) 2187-2194.
7. T. Friedberg, M.A. Grassow, B. Bartlomowicz-Oesch, P. Siegert, M. Arand, M. Adesnik, F. Oesch, Sequence of a novel cytochrome CYP2B cDNA coding for a protein which is expressed in a sebaceous gland, but not in the liver, Biochem. J. 287 (1992) 775-783.
8. N. Ahmad, R. Agarwal, H. Mukhtar, Cytochrome P-450 and drug development for skin diseases, Skin Pharmacol. 9 (1996) 231-241.
9. H. Raza, R. Agarwal, D.R. Bickers, H. Mukhtar, Purification and molecular characterisation of β-naphthoflavone-inducible cyto-chrome P-450 from rat epidermis, J. Invest. Dermatol. 98 (1992) 233-240.
10. I.U. Khan, D.R. Bickers, T.M. Haqqi, H. Mukhtar, Induction of CYP1A1 mRNA in rat epidermis and cultured human epidermal keratinocytes by benz(a)anthracene and β-naphthoflavone, Drug Metab. Dispos. 20 (1992) 620-624.
11. H.J. Einolf, W.T. Story, C.B. Marcus, M.C. Larsen, C.R. Jefcoate, W.F. Greenlee, Role of cytochrome P450 enzyme induction in the metabolic activation of benzo(c)phenanthrene in human cell lines and mouse epidermis, Chem. Res. Toxicol. 10 (1997) 609-617.
12. R.J. Edwards, Targeting antipeptide antibodies towards cytochrome P450 enzymes, in: I.R. Phillips, E.A. Shephard (Eds.), Cytochrome P450 protocols, Totowa, Humana Press, 1998, pp. 239-249.
13. A.G. Riedl, P.M. Watts, R.J. Edwards, A.R. Boobis, P. Jenner, C.D. Marsden, Selective localisation of P450 enzymes and NADPH-P450 oxidoreductase in rat basal ganglia using anti-peptide antisera, Brain Res. 743 (1996) 324-328.
14. A.J. Senam, S.A.J. Beckley-Kartey, S.A.M. Hotchkiss, M. Capel, Comparative in vitro skin absorption and metabolism of coumarin (1,2-benzopyrone) in human, rat and mouse, Toxicol. Appl. Pharmacol. 145 (1997) 34-42.
15. C. Cheung, C.K. Smith, J. Hoog, S.A.M. Hotchkiss, Expression and localization of human alcohol and aldehyde dehydrogenase enzymes in skin, Biochem. Biophys. Res. Commun. 261 (1999) 100-107.
16. K.J. Rich, D. Sesardic, J.R. Foster, D.S. Davies, A.R. Boobis, Immunohistochemical localization of cytochrome P450b/e in hepatic and extrahepatic tissues of the rat, Biochem. Pharmacol. 38(1989) 3305-3322.
17. O.H. Lowry, N.J. Rosebrough, A.L. Farr, R.J. Randal, Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193 (1951) 265-275.
18. T. Schulz-Utermoehl, A.J. Bennett, W. Ellis, G.T. Tucker, A.R. Boobis, R.J. Edwards, Polymorphic debrisoquine 4-hydroxylase activity in the rat is due to differences in CYP2D2 expression, Pharmacogenetics 9 (1999) 357-366.
19. R.J. Edwards, D.A. Adams, P.S. Watts, D.S. Davies, A.R. Boobis, Development of a comprehensive panel of antibodies against the major xenobiotic metabolising forms of cytochrome P450 in human, Biochem. Pharmacol. 56 (1989) 377-387.
20. F.K. Jugert, R. Agarwal, A. Kuhn, D.R. Bickers, H.F. Merk, H. Mukhtar, Multiple cytochrome P450 isozymes in murine skin: induction of P450 1A, 2B, 2E and 3A by dexamethasone, J. Invest. Dermatol. 102 (1994) 970-975.
21. E. Sampol, A. Mirrione, P.H. Villard, P. Piccerelle, H. Scoma, P. Berbis, Y. Barra, Evidence for a tissue-specific induction of cutaneous CYP2E1 by dexamethasone, Biochem. Biophys. Res. Commun. 235 (1997) 557-561.
22. H. Mukhtar, M. Athar, D.R. Bickers, Cytochrome P-450 dependent metabolism of testosterone in rat skin, Biochem. Biophys. Res. Commun. 145 (1987) 749-753.
23. W.A. Khan, S.S. Park, H.V. Gelboin, D.R. Bickers, H. Mukhtar, Epidermal cytochrome P450: immunochemical characterization of isoform induced by topical application of 3-methylcholanthrene to neonatal rat, J. Pharmacol. Exp. Ther. 249 (1989) 921-927.
24. P.G. Zaphiropoulos, S. Westin, A. Strom, A. Mode, J.A. Gustafsson, Structural and regulatory analysis of a cytochrome P450 gene (CYP2C12) expressed predominantly in female rat liver, DNA Cell Biol. 9 (1990) 49-56.
25. H.S. Chun, P.A. Kuzmicky, N.Y. Kado, R.H. Rice, Toxicity of Trp-P-2 to cultured human and rat keratinocytes, Chem. Biol. Interact. 127 (2000) 237-253.