Lipoic acid and vitamin C potentiate nitric oxide synthesis in human aortic endothelial cells independently of cellular glutathione status

Redox Report

Volumn 7, Issue 4, 2002, Pages 223-228

  Visioli, Francesco ^a,b,c   Smith, Anthony ^a   Zhang, Weijian ^a   Keaney, John F ^d   Hagen, Tory ^a   Frei, Balz ^a  

^a OREGON STATE UNIVERSITY   (United States)

^b UNIVERSITY OF MILAN   (Italy)

^c OREGON STATE UNIVERSITY   (United States)

^d BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 OXO 4 THIAZOLIDINECARBOXYLIC ACID; ASCORBIC ACID; CYCLIC GMP; GLUTATHIONE; NITRIC OXIDE; THIOCTIC ACID;

ADRENERGIC SYSTEM; AORTA; ARTICLE; CELL LEVEL; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG ACTIVITY; DRUG MECHANISM; DRUG POTENTIATION; ENDOTHELIUM CELL; HUMAN; HUMAN CELL; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL;

ANTIOXIDANTS; AORTA; ASCORBIC ACID; ENDOTHELIUM, VASCULAR; GLUTATHIONE; HUMANS; NITRIC OXIDE; NITRIC OXIDE SYNTHASE; NITRIC OXIDE SYNTHASE TYPE III; PYRROLIDONECARBOXYLIC ACID; THIAZOLES; THIAZOLIDINES; THIOCTIC ACID;

EID: 0036380213     PISSN: 13510002     EISSN: None     Source Type: Journal    
DOI: 10.1179/135100002125000604     Document Type: Article

References (24)

1. Lamas S, Perez-Sala D, Moncada S. Nitric oxide: from discovery to the clinic. Trends Pharmacol Sci 1998; 19: 436-438.
2. Alheid U, Frolich JC, Forstermann U. Endothelium-derived relaxing factor from cultured human endothelial cells inhibits aggregation of human platelets. Thromb Res 1987; 47: 561-571.
3. Heller R, Polack T, Grabner R, Till U. Nitric oxide inhibits proliferation of human endothelial cells via a mechanism independent of cGMP. Atherosclerosis 1999; 144: 49-57.
4. Selwyn AP, Kinlay S, Ganz P. Atherogenesis and ischemic heart disease. Am J Cardiol 1997; 80: 3H-7H.
5. Gokce N, Keaney Jr JF, Vita JA. Endotheliopathies: clinical manifestations of endothelial dysfunction. In: Loscalzo J, Shafer AI. (eds) Thrombosis and Hemorrhage, 2nd edn. Baltimore, MD: Williams & Wilkins, 1998; 901-924.
6. Levine GN, Frei B, Koulouris SN, Gerhard MD, Keaney Jr JF, Vita JA. Ascorbic acid reverses endothelial vasomotor dysfunction in patients with coronary artery disease. Circulation 1996; 93: 1107-1113.
7. Vita JA, Frei B, Holbrook M, Gokce N, Leaf C, Keaney Jr JF, L-2-Oxothiazolidine-4-carboxylic acid reverses endothelial dysfunction in patients with coronary artery disease J Clin Invest 1998; 101: 1408-1414.
8. Taddei S, Virdis A, Ghiadon L, Magagna A, Salvetti A. Vitamin C improves endothelium-dependent vasodilation by restoring nitric oxide activity in essential hypertension. Circulation 1998; 97: 2222-2229.
9. Gokce N, Keaney Jr JF, Frei B et al. Long-term ascorbic acid administration reverses endothelial vasomotor dysfunction in patients with coronary artery disease. Circulation 1999; 99: 3234-3240.
10. Prasad A, Andrews NP, Padder FA, Husain M, Quyyumi AA. Glutathione reverses endothelial dysfunction and improves nitric oxide bioavailability. J Am Coll Cardiol 1999; 34: 507-514.
11. Ma XL, Lopez BL, Liu GL et al. Hypercholesterolemia impairs a detoxification mechanism against peroxynitrite and renders the vascular tissue more susceptible to oxidative injury. Circ Res 1997; 80: 894-901.
12. Ghigo D, Alessio P, Foco A et al. Nitric oxide synthesis is impaired in glutathione-depleted human umbilical vein endothelial cells. Am J Physiol 1993; 265: C728-C732.
13. Huang A, Vita J, Venema RC, Keaney Jr JF. Ascorbic acid enhances endothelial nitric-oxide synthase activity by increasing intracellular tetrahydrobiopterin. J Biol Chem 2000; 275: 17399-17406.
14. Martin J, White IN. Fluorimetric determination of oxidised and reduced glutathione in cells and tissues by high-performance liquid chromatography following derivatization with dansyl chloride. J Chromatogr 1991; 568: 219-225.
15. Freisleben H. Lipoic acid reduces ischemia-reperfusion injury in animal models. Toxicology 2000; 148: 159-171.
16. Heller R, Münscher-Paulig F, Grabner R, Till U. L-Ascorbic acid potentiates nitric oxide synthesis in endothelial cells. J Biol Chem 1999; 274: 8254-8260.
17. Carr AC, Zhu BZ, Frei B. Potential anti-atherogenic mechanisms of ascorbate (vitamin C) and alpha-tocopherol (vitamin E). Circ Res 2000; 87: 349-354.
18. Murphy ME, Piper HM, Watanabe H, Sies H. Nitric oxide production by cultured aortic endothelial cells in response to thiol depletion and replenishment. J Biol Chem 1991; 266: 19378-19383.
19. Huang A, Xiao H, Samii JM, Vita JA, Keaney Jr JF. Contrasting effects of thiol-modulating agents on endothelial NO bioactivity. Am J Physiol 2001; 281: C719-C725.
20. Sherman DL, Keaney Jr IF, Biegelsen ES, Duffy SJ, Coffman JD, Vita JA. Pharmacological concentrations of ascorbic acid are required for the beneficial effect on endothelial vasomotor function in hypertension. Hypertension 2000; 35: 936-941.
21. Hofmann H, Schmidt HH. Thiol dependence of nitric oxide synthase. Biochemistry 1995; 34: 13443-13452.
22. Stamler JS, Singel DJ, Loscalzo J. Biochemistry of nitric oxide and its redox-activated forms. Science 1992; 258: 1898-1902.
23. Heller R, Unbehaun A, Schellenberg B, Mayer B, Werner-Felmayer G, Werner ER. L-Ascorbic acid potentiates endothelial nitric oxide synthesis via a chemical stabilization of tetrahydrobiopterin. J Biol Chem 2001; 276: 40-47.
24. Thoth M, Kukor Z, Valent S. Chemical stabilization of tetrahydrobiopterin by L-ascorbic acid: contribution to placental endothelial nitric oxide synthase activity. Mol Hum Reprod 2002; 8:271-280.