Extraction of human nuclear DNA from feces samples using the QIAamp DNA stool mini kit

Journal of Forensic Sciences

Volumn 47, Issue 5, 2002, Pages 993-995

  Vandenberg, Nicholas ^a   Van Oorschot, Roland A H ^a  

^a Victoria Forensic Science Centre   (Australia)

Author keywords

DNA extraction; Environmental study; Feces sample; Forensic science; Genotyping; Polymerase chain reaction inhibitors

Indexed keywords

CELL NUCLEUS DNA;

ARTICLE; CONTROLLED STUDY; DNA EXTRACTION; ENVIRONMENTAL FACTOR; ENVIRONMENTAL IMPACT ASSESSMENT; FECES ANALYSIS; FORENSIC SCIENCE; GENE AMPLIFICATION; GENETIC VARIABILITY; GENOTYPE; HUMAN; NORMAL HUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STOCHASTIC MODEL;

EID: 0036378370     PISSN: 00221198     EISSN: None     Source Type: Journal    
DOI: 10.1520/jfs15502j     Document Type: Article

References (14)

1. Monteiro L, Bonnemaison D, Vekris A, Petry KG, Bonnet J, et al. Complex polysaccharides as PCR inhibitors in feces. J Clin Microbiol 1997:995-8.
2. Deuter R, Pietsch S, Hertel S, Muller O. A method for preparation of fecal DNA suitable for PCR. Nucleic Acids Res 1995:23(18):3800-1.
3. Victoria Forensic Science Centre. The preparation of DNA for amplification by the polymerase chain reaction. VFSC internal methods manual 2000.
4. Hochmeister M, Budowle B, Borer U, Eggmann U, Comey C, et al. Typing of deoxyribonucleic acid (DNA) extracted from compact bone from human remains. J Forensic Sci 1991;36:1649-61.
5. Walsh P, Metzger D, Higuchi R. Chelex 100 as a medium for simple extraction of DNA for PCR based typing from forensic material. Biotechniques 1991;10:506-13.
6. Surace D. Retrieval of nuclear DNA from human feces: Implications and techniques in forensic science [hons. thesis]. La Trobe University 1999.
7. QIAGEN. QIAamp DNA Stool Mini Kit handbook 2000:6.
8. PE Applied Biosystems. AmpFISTR Profiler Plus PCR Amplification kit. Users manual 1997.
9. Lantz P-G, Matsson M, Wadstrom T, Radstrom P. Removal of PCR inhibitors from human fecal samples through the use of an aqueous twophase system for sample preparation prior to PCR. J Micro Methods 1997;28:159-67.
10. Launhardt K, Epplen C, Epplen J, Winkler P. Amplification of microsatellites adapted from human systems in fecal DNA of wild Hanuman langurs (Presbytis entellus). Electrophoresis 1998;19:1356-61.
11. Taberlet P, Camarra J-J, Griffin S, Uhres E, Hanotte O, et al. Noninvasive genetic tracking of the endangered Pyrenean brown bear population. Mol Ecol 1997;6:869-76.
12. Shankaranarayanan P, Singh L. A rapid and simplified procedure for isolating DNA from scat samples. Curr Sci 1998:75:883-4.
13. Clement B, Kitts C. Isolating PCR-quality DNA from human feces with a soil DNA kit. BioTechniques 2000;28:640-6.
14. Hopwood A, Mannucci A, Sullivan K. DNA typing from human feces. Int J Leg Med 1996;108:237-43.