Newly induced β-galactosidase molecules have a higher activity than the basally expressed enzyme

Journal of Clinical Laser Medicine and Surgery

Volumn 18, Issue 4, 2000, Pages 209-213

  Craig, D B ^a   Hall, T ^a  

^a UNIVERSITY OF WINNIPEG   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAYS; CELL IMMOBILIZATION; ELECTROPHORESIS; ENZYMES;

BASALLY EXPRESSED ENZYMES; GALACTOSIDASE MOLECULES;

LASER APPLICATIONS;

BETA GALACTOSIDASE; ISOPROPYL BETA THIOGALACTOSIDE; PYRANOSIDE; UNCLASSIFIED DRUG;

ARTICLE; CAPILLARY ELECTROPHORESIS; CATALYSIS; CYTOLYSIS; ENZYME ACTIVITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; NONHUMAN; PROTEIN EXPRESSION;

EID: 0034253211     PISSN: 10445471     EISSN: None     Source Type: Journal    
DOI: 10.1089/10445470050144065     Document Type: Article

References (21)

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3. Isoenzymes of the lactose operon of Escherichia coli
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7. Differences in the chemical reactivity of individual molecules of an enzyme
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9. Life and death of a single enzyme molecule
10. Single molecules of highly purified bacterial alkaline phosphatase have identical activity
11. Single-molecule enzymology
12. E. coli β-galactosidase is heterogeneous with respect to the activity of individual molecules
13. E. coli β-galactosidase is heterogeneous with respect to a requirement for magnesium
14. Studies on the induction of β-galactosidase in a cryptic strain of Escherichia coli
15. The initial kinetics of enzyme induction
16. Detection of attomolar concentrations of alkaline phosphatase by capillary electrophoresis using laser-induced fluorescence detection
17. Picomolar assay of native proteins by capillary electrophoresis - Precolumn labelling, sub-micellar separation, and laser-induced fluorescence detection
18. Yoctomolar detection limit by laser-induced fluorescence in capillary electrophoresis
19. Single-molecule detection in capillary electrophoresis: Molecular shot noise as a fundamental limit to chemical analysis
20. Detection of 100 molecules of product formed in a fucosyltransferase reaction
21. β-Galactosidase assay using capillary electrophoresis laser-induced fluorescence detection and resorufin-beta-D-galactopyranoside as substrate