High affinity of endonuclease VII for the holliday structure containing one nick ensures productive resolution

Journal of Molecular Biology

Volumn 321, Issue 1, 2002, Pages 21-28

  Birkenbihl, Rainer P ^a   Kemper, Börries ^b  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b UNIVERSITY OF COLOGNE   (Germany)

Author keywords

Endonuclease VII; Holliday structure; Reaction mechanism; Recombination; Resolvase

Indexed keywords

DOUBLE STRANDED DNA; ENDONUCLEASE; ENZYME;

ARTICLE; COMPLEX FORMATION; DNA STRAND; HOLLIDAY JUNCTION; PRIORITY JOURNAL; REACTION TIME;

EID: 0036349876     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(02)00594-6     Document Type: Article

References (24)

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5. The junction-resolving enzymes
6. T4 endonuclease VII selects and alters the structure of the four-way DNA junction; binding of a resolution-defective mutant enzyme
7. Localization and characterization of the dimerization domain of Holliday structure resolving endonuclease VII of phage T4
8. Endonuclease VII has two DNA-binding sites each composed from one N- and one C-terminus provided by different subunits of the protein dimer
9. X-ray structure of T4 endonuclease VII: A DNA junction resolvase with a novel fold and unusual domain-swapped dimer architecture
10. Conformational flexibility in T4 endonuclease VII revealed by crystallography: Implications for substrate binding and cleavage
11. T4 endonuclease VII - Importance of a histidine-aspar-tate cluster within the zinc-binding domain
12. Identification of amino acids of endonuclease VII essential for binding and cleavage of cruciform DNA
13. The RuvC protein dimer resolves Holliday junctions by a dual incision mechanism that involves base-specific contacts
14. Ensuring productive resolution by the junction-resolving enzyme RuvC: Large enhancement of the second-strand cleavage rate
15. Yeast resolving enzyme CCE1 makes sequential cleavages in DNA junctions within the lifetime of the complex
16. The junction-resolving enzyme T7 endonuclease 1. Quaternary structure and interaction with DNA
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19. Exchange between stacking conformers in a four-way DNA junction
20. Crossover isomer bias is the primary sequence-dependent property of immobilized Holliday junctions
21. Model for the interaction of DNA junctions and resolving enzymes
22. Cleavage of double-crossover molecules by T4 endo-nuclease VII
23. The stereochemistry of a four-way DNA junction: A theoretical study
24. Use of T7 RNA polymerase to direct expression of cloned genes