Transit Peptide Mutations That Impair in Vitro and in Vivo Chloroplast Protein Import Do Not Affect Accumulation of the γ-Subunit of Chloroplast ATPase

Plant Physiology

Volumn 116, Issue 3, 1998, Pages 1179-1190

  Kindle, Karen L ^a   Lawrence, Susan D ^a,b  

^a Cornell University ^*  (United States)

^b OHIO STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTON TRANSPORTING ADENOSINE TRIPHOSPHATASE; SIGNAL PEPTIDE; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BACTERIUM TRANSFORMATION; CHLAMYDOMONAS REINHARDTII; CHLOROPLAST; ENZYMOLOGY; GENE DOSAGE; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHENOTYPE; PHYSIOLOGY; PLASMID; SITE DIRECTED MUTAGENESIS; TRANSPORT AT THE CELLULAR LEVEL;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BIOLOGICAL TRANSPORT; CHLAMYDOMONAS REINHARDTII; CHLOROPLASTS; GENE DOSAGE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHENOTYPE; PLANT PROTEINS; PLASMIDS; PROTEIN SORTING SIGNALS; PROTON-TRANSLOCATING ATPASES; TRANSFORMATION, BACTERIAL;

EID: 0032015032     PISSN: 00320889     EISSN: None     Source Type: Journal    
DOI: 10.1104/pp.116.3.1179     Document Type: Article

References (53)

1. Archer EK, Keegstra K (1993) Analysis of chloroplast transit peptide function using mutations in the carboxyl-terminal region. Plant Mol Biol 23: 1105-1115
2. Bassham DC, Creighton AM, Karnauchov I, Herrmann RG, Klösgen RB, Robinson C (1994) Mutations at the stromal processing peptidase cleavage site of a thylakoid lumen protein precursor affect the rate of processing, but not the fidelity. J Biol Chem 269: 16062-16066
3. Belknap WR (1983) Partial purification of intact chloroplasts from Chlamydomonas reinhardtii. Plant Physiol 72: 1130-1132
4. Church G, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81: 1991-1995
5. Clark SE, Lamppa GK (1991) Determinants for cleavage of the chlorophyll a/b binding protein precursor: a requirement for a basic residue that is not universal for chloroplast imported proteins. J Cell Biol 114: 681-688
6. Cline K, Henry R (1996) Import and routing of nucleus-encoded proteins. Annu Rev Cell Dev Biol 12: 1-26
7. de Boer D, Bakker H, Lever A, Bouma T, Salentijn E, Weisbeek P (1991) Protein targeting towards the thylakoid lumen of chloroplasts. Proper localization of fusion proteins is only observed in vivo. EMBO J 10: 2765-2772
8. de Boer AD, Weisbeek PJ (1991) Chloroplast protein topogenesis: import, sorting, and assembly. Biochim Biophys Acta 1071: 221-253
9. Debuchy R, Purton S, Rochaix J-D (1989) The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus. EMBO J 8: 2803-2809
10. Drapier D, Girard-Bascou J, Wollman F-A (1992) Evidence for nuclear control of the expression of the atpA and atpB chloroplast genes in Chlamydomonas. Plant Cell 4: 283-295
11. Dumas P, Bergdoll M, Cagnon C, Masson JM (1994) Crystal structure and site-directed mutagenesis of a bleomycin resistance protein and their significance for drug sequestering. EMBO J 13: 2483-2492
12. Durrant I (1990) Light-based detection of biomolecules. Nature 346: 297-298
13. Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6-13
14. Fernández E, Schnell R, Ranum LPW, Hussey SC, Silflow CD, Lefebvre PA (1989) Isolation and characterization of the nitrate reductase structural gene of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 86: 6449-6453
15. Franzén L-G, Rochaix J-D, von Heijne G (1990) Chloroplast transit peptides from the green alga Chlamydomonas reinhardtii share features with both mitochondrial and higher plant chloroplast presequences. FEBS Lett 260: 165-168
16. +-ATPaSe): results by combined biochemical and molecular biological approaches. Annu Rev Biochem 58: 111-136
17. Goldschmidt-Clermont M, Malnöe P, Rochaix J-D (1989) Preparation of Chlamydomonas chloroplasts for the in vitro import of polypeptide precursors. Plant Physiol 89: 15-18
18. Gray JC, Row PE (1995) Protein translocation across chloroplast envelope membranes. Trends Cell Biol 5: 243-247
19. Harris EH (1989) The Chlamydomonas Sourcebook: A Comprehensive Guide to Biology and Laboratory Use. Academic Press, San Diego, CA
20. Junge W (1989) Protons, the thylakoid membrane, and the chloroplast ATP synthase. Ann NY Acad Sci 574: 268-286
21. Keegstra K (1989) Transport and routing of proteins into chloroplasts. Cell 56: 247-253
22. Keegstra K, Olsen LJ, Theg SM (1989) Chloroplastic precursors and their transport across envelope membranes. Annu Rev Plant Physiol Plant Mol Biol 40: 471-501
23. Kindle KL (1990) High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 87: 1228-1232
24. Kindle KL, Schnell RA, Fernández E, Lefebvre PA (1989) Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase. J Cell Biol 109: 2589-2601
25. Kunkel TA, Bebenek K, McClary J (1991) Efficient site-directed mutagenesis using uracil-containing DNA. Methods Enzymol 204: 125-139
26. Kuntz M, Simons J, Schell J, Schreier PH (1986) Targeting of proteins to chloroplasts in transgenic tobacco by fusion to mutated transit peptides. Mol Gen Genet 205: 454-460
27. Lawrence SD, Kindle KL (1997) Alterations in the Chlamydomonas plastocyanin transit peptide have distinct effects on in vitro import and in vivo protein accumulation. J Biol Chem 272: 20357-20363
28. McCarty RE, Carmeli C (1982) Proton translocating ATPase of photosynthetic membranes. In Govindjee, ed, Photosynthesis: Energy Conversion by Plants and Bacteria, Vol 1. Academic Press, New York, pp 647-695
29. Merchant S, Hill K, Kim JH, Thompson J, Zaitlin D, Bogorad L (1990) Isolation and characterization of a complementary DNA clone for algal pre-apoplastocyanin. J Biol Chem 265: 12372-12379
30. Merchant S, Selman B (1984) Synthesis and turnover of the chloroplast coupling factor 1 in Chlamydomonas reinhardtii. Plant Physiol 75: 781-787
31. Ostrem JA, Rammage RT, Bohnert HJ, Wasmann CC (1989) Deletion of the carboxyl-terminal portion of the transit peptide affects processing but not import or assembly of the small subunit of ribulose bisphosphate caryboxylase. J Biol Chem 264: 3662-3665
32. Pilon M, Wienk H, Sips W, de Swaaf M, Talboom I, van't Hof R, de Korte-Kool G, Demel R, Weisbeek P, de Kruijff B (1995) Functional domains of the ferredoxin transit sequence involved in chloroplast import. J Biol Chem 270: 3882-3893
33. Quinn J, Li HH, Singer J, Morimoto B, Mets L, Kindle K, Merchant S (1993) The plastocyanin-deficient phenotype of Chlamydomonas ac-208 results from a frame-shift mutation in the nuclear gene encoding preapoplastocyanin. J Biol Chem 268: 7832-7841
34. Quinn JM, Merchant S (1995) Two copper-responsive elements associated with the Chlamydomonas Cyc6 gene function as targets for transcriptional activators. Plant Cell 7: 623-638
35. Reiss B, Wasmann CC, Bohnert HJ (1987) Regions in the transit peptide of SSU essential for transport into chloroplasts. Mol Gen Genet 209: 116-121
36. Robinson C, Klösgen RB (1994) Targeting of proteins into and across the thylakoid membrane - a multitude of mechanisms. Plant Mol Biol 26: 15-24
37. Schnell DJ (1995) Shedding light on the chloroplast protein import machinery. Cell 83: 521-524
38. Silva-Filho M, Wieers M, Flugge U, Chaumont F, Boutry M (1997) Different in vitro and in vivo targeting properties of the transit peptide of a chloroplast envelope inner membrane protein. J Biol Chem 272: 15264-15269
39. Smart EJ, Selman BR (1991a) Isolation and characterization of a Chlamydomonas reinhardtii mutant lacking the γ-subunit of chloroplast coupling factor 1 (CF1). Mol Cell Biol 11: 5053-5058
40. Smart EJ, Selman BR (1991b) Nucleotide sequence for the genomic DNA encoding the chloroplast ATPase γ-subunit gene (atpC) from Chlamydomonas reinhardtii. Plant Physiol 97: 1596-1598
41. 1) γ-subunit (atpC). J Bioenerg Biomembr 25: 275-284
42. Smeekens S, Bauerle C, Hageman J, Keegstra K, Weisbeek P (1986) The role of the transit peptide in the routing of precursors toward different chloroplast compartments. Cell 46: 365-376
43. Sodeinde OA, Kindle KL (1993) Homologous recombination in the nuclear genome of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 90: 9199-9203
44. Stern DB, Radwanski ER, Kindle KL (1991) A 3′ stem/loop structure of the Chlamydomonas chloroplast atpB gene regulates mRNA accumulation in vivo. Plant Cell 3: 285-297
45. Stevens DR, Rochaix J-D, Purton S (1996) The bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas. Mol Gen Genet 251: 23-30
46. Tam L-W, Lefebvre PA (1993) The use of DNA insertional mutagenesis to clone genes in Chlamydomonas. Genetics 135: 375-384
47. Theg SM, Geske FJ (1992) Biophysical characterization of a transit peptide directing chloroplast protein import. Biochemistry 31: 5053-5060
48. Theg SM, Scott SV (1993) Protein import into chloroplasts. Trends Cell Biol 3: 186-190
49. von Heijne G, Steppuhn J, Herrmann RG (1989) Domain structure of mitochondrial and chloroplast targeting peptides. Eur J Biochem 180: 535-545
50. Wasmann CC, Reiss B, Bartlett SG, Bohnert HJ (1986) The importance of the transit peptide and the transported protein for protein import into chloroplasts. Mol Gen Genet 205: 446-453
51. Wasmann CC, Reiss B, Bohnert HJ (1988) Complete processing of a small subunit of ribulose-1,5-bisphosphate carboxylase-oxygenase from pea requires the amino acid sequence ile-thrser. J Biol Chem 263: 617-619
52. Yu LM, Merchant S, Theg SM, Selman BR (1988) Isolation of a complementary DNA clone for the gamma subunit of the chloroplast ATPase of Chlamydomonas reinhardtii. Import and cleavage of the precursor protein. Proc Natl Acad Sci USA 85: 1369-1373
53. Yu LM, Selman BR (1988) cDNA sequence and predicted primary structure of the γ subunit from the ATP synthase from Chlamydomonas reinhardtii. J Biol Chem 263: 19342-19345