Inhibitory effects of agarose gel and LB medium on DNA sequencing

BioTechniques

Volumn 33, Issue 2, 2002, Pages 282-284

  Yamaguchi, Yukihiro ^a   Nimbari, S ^a   Ookawara, T ^a   Oishi, K ^a   Eguchi, H ^a   Suzuki, K ^a  

^a HYOGO COLLEGE OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AGAROSE; DNA POLYMERASE; BACTERIAL DNA; DNA DIRECTED DNA POLYMERASE; SEPHAROSE; VIRUS DNA;

ARTICLE; CONCENTRATION (PARAMETERS); DNA LIBRARY; DNA SEQUENCE; DNA TEMPLATE; GENE INSERTION; POLYMERASE CHAIN REACTION; BACTERIOPHAGE LAMBDA; CHEMISTRY; CULTURE MEDIUM; DOSE RESPONSE; DRUG ANTAGONISM; DRUG EFFECT; EQUIPMENT; EVALUATION; GENETICS; INSTRUMENTATION; METHODOLOGY; PLASMID; QUALITY CONTROL; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY;

INSERTION SEQUENCES;

BACTERIOPHAGE LAMBDA; CULTURE MEDIA; DNA, BACTERIAL; DNA, VIRAL; DNA-DIRECTED DNA POLYMERASE; DOSE-RESPONSE RELATIONSHIP, DRUG; EQUIPMENT FAILURE ANALYSIS; PLASMIDS; POLYMERASE CHAIN REACTION; QUALITY CONTROL; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SEPHAROSE; SEQUENCE ANALYSIS, DNA;

EID: 0036334106     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02332bm05     Document Type: Article

References (7)

1. Purification and characterization of PCR-inhibitory components in blood cells
2. Rapid screening of plasmid DNA by direct sequencing from bacterial colonies
3. Inhibition of PCR by agar from bacteriological transport media
4. Dye-terminator re-cycle-sequencing method: Phage plaque direct sequencing
5. Direct colony sequencing of plasmid DNA by dye terminator methods
6. Colony and phage plaque direct sequencings by dyeterminator method
7. Ready to use agarose encapsulated PCR reagents