Scanning mutagenesis using T4 DNA ligase and short degenerate DNA oligonucleotides containing tri-nucleotide mismatches

Journal of Biochemistry

Volumn 132, Issue 1, 2002, Pages 143-147

  Cherepanov, Alexei V ^a   De Vries, Simon ^a  

^a DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

Author keywords

Nick ligation; Oligonucleotides; Scanning mutagenesis; T4 DNA ligase

Indexed keywords

DNA; DNA POLYMERASE; DOUBLE STRANDED DNA; EXONUCLEASE; OLIGONUCLEOTIDE; PHOSPHATE; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; POLYNUCLEOTIDE 5' HYDROXYL KINASE; TRINUCLEOTIDE; DNA DIRECTED DNA POLYMERASE; EXODEOXYRIBONUCLEASE; EXODEOXYRIBONUCLEASE III; OLIGODEOXYRIBONUCLEOTIDE; PRIMER DNA;

ARTICLE; BASE MISPAIRING; BINDING SITE; DNA STRUCTURE; DNA SYNTHESIS; DNA TEMPLATE; MUTAGENESIS; PROTEIN STRUCTURE; STRUCTURE ACTIVITY RELATION; BACTERIOPHAGE T4; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; PHYSIOLOGY;

BACTERIOPHAGE T4; BASE PAIR MISMATCH; BINDING SITES; DNA; DNA LIGASES; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; EXODEOXYRIBONUCLEASES; KINETICS; MUTAGENESIS; OLIGODEOXYRIBONUCLEOTIDES; POLYNUCLEOTIDE 5'-HYDROXYL-KINASE; SUBSTRATE SPECIFICITY;

EID: 0036330503     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a003192     Document Type: Article

References (13)

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13. DNA sequencing with chain-terminating inhibitors