Structural determinants of Ras-Raf interaction analyzed in live cells

Molecular Biology of the Cell

Volumn 13, Issue 7, 2002, Pages 2323-2333

  Bondeva, Tzvetanka ^a   Balla, András ^a   Várnai, Péter ^a   Balla, Tamas ^a  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE KINASE KINASE; PHORBOL 13 ACETATE 12 MYRISTATE; PLATELET DERIVED GROWTH FACTOR; PROTEIN SERINE THREONINE KINASE; RAF PROTEIN; RAS PROTEIN; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; MAP2K1 PROTEIN, MOUSE; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; PHOTOPROTEIN; PLATELET DERIVED GROWTH FACTOR AB; PLATELET-DERIVED GROWTH FACTOR AB;

ANIMAL CELL; ARTICLE; CELL MEMBRANE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME ACTIVATION; GENE OVEREXPRESSION; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; STRUCTURE ANALYSIS; ANIMAL; BINDING SITE; CELL STRAIN 3T3; CELL STRAIN COS1; CELL SURFACE; CONFOCAL MICROSCOPY; GENETICS; METABOLISM; MOUSE; MUTATION; PROTEIN TERTIARY STRUCTURE;

ANIMALIA;

3T3 CELLS; ANIMALS; BINDING SITES; CELL SURFACE EXTENSIONS; COS CELLS; ENZYME ACTIVATION; GREEN FLUORESCENT PROTEINS; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; MAP KINASE KINASE 1; MICE; MICROSCOPY, CONFOCAL; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE KINASES; MUTATION; PLATELET-DERIVED GROWTH FACTOR; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEIN-SERINE-THREONINE KINASES; PROTO-ONCOGENE PROTEINS C-RAF; RAS PROTEINS; RECOMBINANT FUSION PROTEINS;

EID: 0036325229     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.E02-01-0019     Document Type: Article

References (39)

1. Raf meets Ras: Completing the framework of a signal transduction pathway
2. Bifurcation of lipid and protein kinase signals of PI3K3γ to the protein kinases PKB and MAPK
3. Two distinct Raf domains mediate interaction with Ras
4. Hydrolysis of phosphatidylcholine couples Ras activation of Raf protein kinase during mitogenic signal transduction
5. Endomembrane trafficking of Ras: The CAAX motif targets proteins to the ER and Golgi
6. Regulation of Raf-1 activation and signaling by dephosphorylation
7. Ras interaction with two distinct binding domains in Raf-1 may be required for Ras transformation
8. Critical tyrosine residues regulate the enzymatic and biological activity of Raf-1 kinase
9. Sequential modification of serines 621 and 624 in the Raf-1 carboxyl terminus produces alterations in its electrophoretic mobility
10. Binding of 14-3-3 proteins to the protein kinase Raf and effects on its activation
11. Equilibrium and kinetic measurements reveal rapidly reversible binding of Ras to Raf
12. Cysteine-rich region of Raf-1 interacts with activator domain of post-translationally modified Ha-Ras
13. Cysteine-rich region of Raf-1 interacts with activator domain of post-translationally modified Ha-Ras
14. Ceramide-binding and activation defines protein kinase c-Raf as a ceramide-activated protein kinase
15. The binding site for βγ subunits of heterotrimeric G proteins on the β-adrenergic receptor kinase
16. An intact Raf zinc finger is required for optimal binding to processed Ras and for ras-dependent Raf activation in situ
17. Interaction of c-Raf-1 with phosphatidylserine and 14-3-3
18. Crystal structure of a phosphatidylinositol 3-phosphate-specific membrane-targeting motif, the FYVE domain of Vps27p
19. Spatio-temporal images of growth factor-induced activation of Ras and Rap1
20. The complexity of Raf-1 regulation
21. The solution structure of the Raf-1 cysteinerich domain: A novel Ras and phospholipid binding site
22. The 2.2A crystal structure of the Ras-binding domain of the serine/threonine kinase cRaf1 in complex with Rap1A and a GTP analogue
23. Structural basis of Rab effector specificity: Crystal structure of the small GTP protein Rab3A complexed with the effector domain of rabphilin-3A
24. A direct interaction between G-protein β γ subunits and the Raf-1 protein kinase
25. Phospholipase D and its product, phosphatidic acid mediate agonist-dependent Raf-1 translocation to the plasma membrane and the activation of the mitogen-activated protein kinase pathway
26. Agonist-dependent traffic of raft-associated Ras and Raf-1 is required for activation of the mitogen-activated protein kinase cascade
27. The recruitment of Raf-1 to membranes is mediated by direct interaction with phosphatidic acid and is independent of association with Ras
28. Role of phosphoinositide 3-OH kinase in cell transformation and control of the actin cytoskeleton by Ras
29. Activity of plasma membrane-recruited Raf-1 is regulated by Ras via the Raf zinc finger
30. Dominant-negative caveolin inhibits H-Ras function by disrupting cholesterol-rich plasma membrane domains
31. Activation of Raf as a result of recruitment to the plasma membrane
32. Translocation and reversible localization of signaling proteins: A dynamic future for signal transduction
33. Binding of the βγ subunits of heterotrimeric G-proteins to the PH domain of Bruton's tyrosine kinase
34. 3H]inositol-labeled phosphoinositide pools
35. Phosphatidylinositol 3-kinase-dependent membrane association of the Bruton's tyrosine kinase pleckstrin homology domain visualized in single living cells
36. Mammalian Ras interacts with the serine/threonine kinase Raf
37. Bcl-2 targets the protein kinase Raf-1 to mitochondria
38. Elucidation of binding determinants and functional consequences of Ras/Raf-CRD interactions
39. Crystal structure of the cys2 activator-binding domain of protein kinase Cδ in complex with phorbol ester