Efficient transient transformation of suspension culture-derived apple protoplasts

Plant Cell, Tissue and Organ Culture

Volumn 70, Issue 1, 2002, Pages 77-82

  Maddumage, Ratnasiri ^a   Fung, Raymond M W ^a   Weir, Iona ^a   Ding, Hong ^a   Simons, Joanne L ^a   Allan, Andrew C ^a  

^a THE NEW ZEALAND INSTITUTE FOR PLANT AND FOOD RESEARCH LIMITED   (New Zealand)

Author keywords

Flow cytometry; Green fluorescent protein; Malus

Indexed keywords

DNA;

CYTOMETRY;

FRUITS;

MALUS; MALUS X DOMESTICA;

EID: 0036320278     PISSN: 01676857     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1016073611902     Document Type: Conference Paper

References (17)

1. High efficiency transformation of cultured tobacco cells
2. PEG-mediated direct gene transfer and electroporation
3. Control of quiescence and viability in auxin deprived pear cells in batch and continuous culture
4. Plant regeneration from cotyledon and cell suspension protoplasts of apple (Malus x domesttca cv. Starkrimson)
5. Transient expression in Arabidopsis thaliana protoplasts derived from rapidly established cell suspension cultures
6. Stable transformation of an Arabidopsis cell suspension culture with firefly luciferase providing a cellular system for analysis of chaperone activity in vivo
7. A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome
8. Agrobacterium-mediated transformation of hybrid poplar suspension culture and regeneration of transformed plants
9. Foreign gene transfer using electroporation and transient expression in apple (Malus domestica Borkh.)
10. Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants
11. Investigation of Agrobacterium-mediated transformation of apple using green fluorescent protein: High transient expression and low stable transformation suggest that factors other than T-DNA transfer are rate-limiting
12. Plant regeneration from protoplasts of apple rootstocks and scion varieties (Malus x domestica)
13. A general strategy for the isolation of mesophyll protoplasts from deciduous fruit and nut tree species
14. 2+ dependent protein kinases and stress signal transduction in plants
15. Conditioning promotes regeneration and transformation in apple leaf explants
16. Green fluorescent protein as a tool for monitoring transgene expression in forest tree species
17. Regeneration of transgenic plants from the commercial apple cultivar Royal Gala