SCOPUS 정보 검색 플랫폼

Science

Volumn 274, Issue 5294, 1996, Pages 1900-1902

Ca2+-dependent protein kinases and stress signal transduction in plants

(1) Sheen, Jen a

a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ABSCISIC ACID; CALPAIN; PHOSPHOPROTEIN PHOSPHATASE; ARABIDOPSIS PROTEIN; ATCDPK1 PROTEIN, ARABIDOPSIS; CALCIUM; CALCIUM BINDING PROTEIN; CDPK1 PROTEIN, PLANT; COMPLEMENTARY DNA; GREEN FLUORESCENT PROTEIN; HVA1 PROTEIN, HORDEUM VULGARE; PHOSPHOPROTEIN PHOSPHATASE 2C; PHOTOPROTEIN; PROTEIN KINASE; PTC1 PROTEIN, S CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEIN; VEGETABLE PROTEIN;

ARTICLE; ENZYME ACTIVATION; GENE MUTATION; MAIZE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; SIGNAL TRANSDUCTION; STRESS; AMINO ACID SEQUENCE; ARABIDOPSIS; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; GENETICS; METABOLISM; MOLECULAR GENETICS; PLANT LEAF; PROTOPLAST; REPORTER GENE; SITE DIRECTED MUTAGENESIS;

ABSCISIC ACID; AMINO ACID SEQUENCE; ARABIDOPSIS; ARABIDOPSIS PROTEINS; CALCIUM; CALCIUM-BINDING PROTEINS; DNA, COMPLEMENTARY; GENE EXPRESSION REGULATION, PLANT; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHOSPHOPROTEIN PHOSPHATASE; PLANT LEAVES; PLANT PROTEINS; PROMOTER REGIONS (GENETICS); PROTEIN KINASES; PROTOPLASTS; SACCHAROMYCES CEREVISIAE PROTEINS; SIGNAL TRANSDUCTION; TRANSFECTION; ZEA MAYS;

EID: 0030300175 PISSN: 00368075 EISSN: None Source Type: Journal
DOI: 10.1126/science.274.5294.1900 Document Type: Article

Times cited : (502)

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- Kapitoff, M.S.¹

59
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- note
- Various PK cDNAs were obtained by PCR with the use of an Aratidopsis cDNA library. The primers were as follows: AK1 or ATCDPK, 5′-GAAGATCTATGGGTAATACTTGTGTTGGA-3′ and 5′-GTCAAGGCCTGTCGACTTGAACCCATGG-3′; ATCDPK1 and ATCDPK1a, 5′-GCGGATCCATGGCTAATCAAACTCAGATCAGCG-3′ and 5′-GTCAAGGCCTCATCAGTGAGAACATGTTC-3′; ATCDPK2, 5′-GCGGATCCATGGAGACGAAGCCAMCCCTA-3′ and 5′-GTCAAGGCCTTGCTTGTTCATCGACAATCC-3′; ATPKa, 5′-CATGCCATGGCTCCGGCGACTAATTCACCG-3′ and 5′-GTCAAGGCCTATTCTTCAAGAACCATTTATCG-3′; ATP-Kb, 5′-GCGGATCCATGGCTCGAGCTCCGGTGACCA-3′ and 5′-GTCAAGGCCTATTCTTCAAGAACCAACTATG-3′; ASK1, 5′-GCGGATCCATGGCTAAGTCAGAGCTGGTGAAAG-3′ and 5′-GTCAAGGCCTATTCTTTAGGAACCATGAATG-3′; and ASK2, 5′-GCGGATCCATGGCTAAGTATGACGTTGTCAAGG-3′ and GTCAAGGCCTATTCTTCAAGTACCACGG-3′. The sequence of ATCDPK1a was determined for both strands with an automatic sequencing facility.

60
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- Minet, M.¹ Dufour, M.-E.² Lacroute, F.³

61
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62
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- Park, Y.S.¹

63
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- (1995) Plant Physiol. , vol.108 , pp. 1203
- Holappa, L.D.¹ Walker-Simmons, M.K.²

64
- 85033160575
- note
- Each kinase tested was inserted after the 35SC4PPDK promoter and before the DHA tag and nopanine synthase terminator sequence. Kinase fragments used were as follows: AtCDPK (AK1), amino acids 1 through 413; ATCDPK1, amino acids 1 through 274; ATCDPK1a, amino acids 1 through 274; ATCDPK2, amino acids 1 through 289; ATPKa, amino acids 1 through 284; ATPKb, amino acids 1 through 283; ASK1, amino acids 1 through 265; and ASK2, amino acids 1 through 265. One strand of the sequence for the DHA tag was 5′-CCTTACCCATACGACGTTCCAGACTACGCTGGTT-ACCCATACGACGTTCCAGACTACGCT-3′. All cDNAs were fused to the DHA sequence YPYDVPDYAGYPYDVPDYA (single-letter abbreviations for the amino acid residues are: A, Ala; D, Asp; G, Gly; P. Pro; V, Val; and Y, Tyr) at the Stu I site.

65
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- Luehrsen, K.R.¹ De Wet, J.R.² Walbot, V.³

66
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- (1994) Science , vol.264 , pp. 1448
- Leung, J.¹

67
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- Science , pp. 1452
- Meyer, K.¹ Leube, M.P.² Grill, E.³

68
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- (1995) Proc. Natl. Acad. Sci. U.S.A. , vol.92 , pp. 9520
- Armstrong, F.¹

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- (1994) Plant Physiol. , vol.106 , pp. 1241
- Newman, T.¹

70
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- note
- I thank M. Minet for an outstanding Arabidopsis cDNA library, F. Katagiri for the PP2C cDNA, V. Walbot and P. Leon for the LUC vector, P. Quail for the maize UBI promoter, W.-L. Chiu and J.-C. Jang for plasmid DNA, L. Zhou for the plant expression vector with a double HA tag, W. Zeng and L. Zhou for the immunoprecipitation protocol, S. Bamarda for barley genomic DNA, and T. Jones and J. Nardone for critical comments on the manuscript. Supported by USDA and Hoechst A.G.

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